Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Peritonite/complicações , Peritonite , Obstrução Intestinal/patologia , Obstrução Intestinal , Radiografia Abdominal/métodos , Peritônio/patologia , Peritônio , Intestino Delgado/patologia , Intestino Delgado , Abdome/patologia , Abdome , Diagnóstico DiferencialAssuntos
Antagonistas de Receptores Adrenérgicos beta 1/efeitos adversos , Atenolol/efeitos adversos , Fibrose Peritoneal/induzido quimicamente , Peritonite/induzido quimicamente , Humanos , Masculino , Pessoa de Meia-Idade , Fibrose Peritoneal/diagnóstico por imagem , Peritonite/diagnóstico por imagem , Tomografia Computadorizada por Raios XRESUMO
BACKGROUND: Lentils are increasingly consumed in many parts of the world.Two allergens, Len c 1 and 2, have been reported previously. Recently, peanut and green bean lipid transfer proteins (LTPs) have been identified as the first two members of an important group of allergens that might be associated with severe food allergies. OBJECTIVE: To investigate lentil LTP as a potential new allergen. METHODS: Efficacy of LTP extraction was monitored at different acidic pH values, using immunoblotting with cross-reactive anti-peach LTP antiserum. Natural LTP was purified from lentil extract and expressed as recombinant allergen in Escherichia coli. Sera from 10 lentil-allergic and/or -sensitized patients (Spain: 6, Italy: 1 and the Netherlands: 3) were used to further characterize lentil LTP. RESULTS: Natural lentil LTP, purified from the homogenized germinated seeds and optimally extracted at pH 3, was identified and designated as allergen Len c 3. By CAP, 9/10 sera showed specific IgE to Len c 3. Recombinant (r) Len c 3 was successfully purified. The natural (n) Len c 3 CAP was completely inhibited by rLen c 3/rPru p 3. IgE binding to lentil pH 3 extract blot was completely inhibited by rLen c 3. CONCLUSION: The availability of immunochemically active nLen/rLen c 3 as a novel legume allergen facilitates further development and implementation of a third (next to peanut and green bean) legume LTP in component-resolved diagnosis strategies and contributes to evaluate the clinical importance of legume LTPs. Preferential extraction of Len c 3 (pH 3) will affect the production of sensitive extract-based diagnostic tests.
Assuntos
Alérgenos/imunologia , Antígenos de Plantas/imunologia , Proteínas de Transporte/imunologia , Lens (Planta)/imunologia , Proteínas de Plantas/imunologia , Adolescente , Adulto , Criança , Feminino , Hipersensibilidade Alimentar/imunologia , Humanos , Concentração de Íons de Hidrogênio , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Extratos Vegetais , Adulto JovemAssuntos
Alérgenos/imunologia , Dermatite Alérgica de Contato/imunologia , Hipersensibilidade Imediata/imunologia , Larva/imunologia , Lepidópteros/imunologia , Animais , Dermatite Alérgica de Contato/diagnóstico , Feminino , Humanos , Hipersensibilidade Imediata/diagnóstico , Immunoblotting , Testes Cutâneos , Urticária/diagnóstico , Adulto JovemRESUMO
BACKGROUND: Delayed reactions with betalactam antibiotics are a very common reason for consultation and a matter of numerous publications. OBJECTIVE: To demonstrate that delayed reactions occurring during treatment with betalactam antibiotics are not reproduced in a high percentage of the patients, when making drug challenge. To analyse the characteristics of people showing this type of reaction. METHODS: We included in our study all the patients who came to our Allergy Department during one year (2004), with a clinical history of delayed reaction (> 72h) to betalactams. Skin prick tests (SPT), intradermal tests (IT) and patch tests were carried out, followed by simple blind placebo controlled drug challenge (SBPCDC) at hospital and home treatment with betalactams. RESULTS: We studied 23 patients (12 men and 11 women), average age 23.4 years old. SPT and patch test were negative in all patients. Only one patient showed positive IT tests, and allergic reaction was only reproduced in two patients; 76 % tolerated the drug involved in supposed allergy. CONCLUSIONS: Simple blind oral challenge with implicated drug followed by home treatment is required for a conclusive diagnosis of allergy in patients with delayed reactions to betalactams.
Assuntos
Antibacterianos/efeitos adversos , Hipersensibilidade Tardia/diagnóstico , Hipersensibilidade Tardia/imunologia , beta-Lactamas/efeitos adversos , Adolescente , Adulto , Idoso , Antibacterianos/imunologia , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina E/sangue , Lactente , Masculino , Pessoa de Meia-Idade , Testes do Emplastro , Reprodutibilidade dos Testes , Método Simples-Cego , Testes Cutâneos , Adulto Jovem , beta-Lactamas/imunologiaRESUMO
Background: Delayed reactions with betalactam antibiotics are a very common reason for consultationand a matter of numerous publications. Objective: To demonstrate that delayed reactions occurring during treatment with betalactam antibiotics are not reproduced in a high percentage of the patients, when making drug challenge.To analyse the characteristics of people showing this type of reaction. Methods: We included in our study all the patients who came to our Allergy Department during one year (2004), with a clinical history of delayed reaction (> 72h) to betalactams. Skin prick tests (SPT), intradermal tests (IT) and patch tests were carried out, followed by simple blind placebo controlled drug challenge (SBPCDC) at hospital and home treatment with betalactams. Results: We studied 23 patients (12 men and 11 women), average age 23.4 years old. SPT and patch test were negative in all patients. Only one patient showed positive IT tests, and allergic reaction was only reproduced in two patients; 76 % tolerated the drug involved in supposed allergy. Conclusions: Simple blind oral challenge with implicated drug followed by home treatment is required for a conclusive diagnosis of allergy in patients with delayed reactions to betalactams
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