[Detection and genotyping of high-risk human papillomavirus in cervical specimens]. / Detección y genotipado del virus del papiloma humano de alto riesgo en muestras de lesiones cervicales.

INTRODUCTION: This study investigates the relationship between various human papillomavirus (HPV) genotypes and the results of cytological and histological analysis of cervical samples using two complementary assays for HPV detection (hybrid capture and PCR). We studied the impact of HPV genotype on the presence of pre-cancerous cervical lesions and cervical cancer, as well as the association between HPV viral load and the presence of high-risk HPV as determined by PCR. METHODS: A total of 272 women were studied. Most of them presented cellular alterations consistent with cervical lesions due to HPV and all had high-risk HPV as detected by hybrid capture testing. Histological studies were undertaken, and HPV genotyping by PCR based on microarrays was performed. RESULTS: HPV-DNA was not detected or genotypes could not be identified by PCR in 22.06% of the patients. Genotype 16 and/or 18 was detected in 33% of 212 patients. Mixed infections with several genotypes were found in 25% of patients. The histological lesions associated with the various genotypes were as follows: genotype 16 and/or 18. were detected in 55.73% of the 61 patients with H-SIL and cancer, whereas these genotypes were detected in only 7.9% and 22% of women with ASCUS and L-SIL (P < 0.05). Viral load was less than 3 pg/mL in 12.13% of the women studied. In this group of patients, high-risk HPV was present in 39.39%. In the group of patients who had a viral load greater than 3 pg/mL, high risk-HPV was detected in 77.4% (P < 0.05). CONCLUSIONS: Genotypes 16 and/or 18 were detected in most patients with a diagnosis of H-SIL. Other high-risk-HPV genotypes were much less prevalent. Hybrid capture testing is a useful screening test. PCR was effective for identifying genotypes 16 and 18. Histological and cytological findings in cervical samples should be interpreted together with high-risk HPV detection.

Detección y genotipado del virus del papiloma humano de alto riesgo en muestras de lesiones cervicales / Detection and genotyping of high-risk human papillomavirus in cervical specimens

Introducción. El objetivo del presente estudio es utilizando dos técnicas complementarias de detección del virus del papiloma humano (VPH) (captura de híbridos [CH] y reacción en cadena de la polimerasa [PCR]), relacionar el estudio citológico y/o la biopsia de cérvix con la presencia de distintos genotipos del VPH, para conocer la influencia de éstos en la producción de lesiones precancerosas y cáncer cervical, así como relacionar la carga viral con la presencia de VPH-AR (alto riesgo) determinados por PCR. Métodos. Se han estudiado 272 mujeres que presentaban la mayoría alteraciones celulares compatibles con lesiones cervicales por el VPH. En todas se ha detectado VPH de alto riesgo por el método de CH y se realizó estudio histológico y detección del genotipo del VPH por técnica de PCR. Resultados. En el 22,06% de las pacientes no se detectó ADN de VPH con la técnica de PCR. El genotipo 16 y/o 18 fue el prevalente y se encontró en el 33% de las 212 mujeres estudiadas. Se hallaron infecciones mixtas por varios genotipos en el 25% de estas 212 pacientes. En cuanto a las lesiones histológicas encontradas, en 61 pacientes con lesiones escamosas intraepiteliales de alto grado (H-SIL) y cáncer, el 55,73% presentaban genotipos 16 y/o 18, mientras que en 38 pacientes con presencia de células escamosas alteradas de significado incierto (ASCUS) y en 126 con lesiones escamosas intraepiteliales de bajo grado (L-SIL), se pusieron de manifiesto estos genotipos únicamente en el 7,9 y 22,2%, respectivamente, estableciéndose una relación estadísticamente significativa (p 3 pg/ml, se encontró VPH-AR en el 77,40% de éstas, siendo esta relación significativa (p < 0,05). Conclusiones. En las pacientes con biopsia de H-SIL hemos encontrado mayoritariamente la presencia de los genotipos 16 y/o 18. La técnica de CH es útil como procedimiento de cribado. La técnica de PCR es interesante para identificar los genotipos 16 y 18. Es conveniente que los resultados de los tests de detección del VPH se interpreten conjuntamente con los de biopsia y citología (AU)

INTRODUCTION. This study investigates the relationship between various human papillomavirus (HPV) genotypes and the results of cytological and histological analysis of cervical samples using two complementary assays for HPV detection (hybrid capture and PCR). We studied the impact of HPV genotype on the presence of pre-cancerous cervical lesions and cervical cancer, as well as the association between HPV viral load and the presence of high-risk HPV as determined by PCR. METHODS. A total of 272 women were studied. Most of them presented cellular alterations consistent with cervical lesions due to HPV and all had high-risk HPV as detected by hybrid capture testing. Histological studies were undertaken, and HPV genotyping by PCR based on microarrays was performed. RESULTS. HPV-DNA was not detected or genotypes could not be identified by PCR in 22.06% of the patients. Genotype 16 and/or 18 was detected in 33% of 212 patients. Mixed infections with several genotypes were found in 25% of patients. The histological lesions associated with the various genotypes were as follows (..) (AU)