Assuntos
Alérgenos/imunologia , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/imunologia , Adolescente , Adulto , Alérgenos/química , Animais , Testes de Provocação Brônquica , Criança , Cricetinae , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Phodopus/imunologia , Hipersensibilidade Respiratória/induzido quimicamente , Hipersensibilidade Respiratória/patologia , Testes CutâneosRESUMO
No disponible
Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Animais de Estimação/imunologia , Phodopus/imunologia , Alergia e Imunologia , Hipersensibilidade/complicações , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Conjuntivite/complicações , Conjuntivite/imunologia , Conjuntivite Alérgica/imunologia , Mesocricetus/imunologia , Obstrução Nasal/complicações , Obstrução Nasal/imunologia , Rinite/complicações , Rinite/imunologiaAssuntos
Alérgenos/imunologia , Antígenos de Plantas/imunologia , Hipersensibilidade Alimentar/diagnóstico , Proteínas de Plantas/imunologia , Salvia/imunologia , Sementes/imunologia , Alérgenos/análise , Anafilaxia/sangue , Anafilaxia/diagnóstico , Anafilaxia/imunologia , Antígenos de Plantas/análise , Hipersensibilidade Alimentar/sangue , Hipersensibilidade Alimentar/imunologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Extratos Vegetais/química , Proteínas de Plantas/análise , Sementes/químicaRESUMO
No disponible
Assuntos
Humanos , Masculino , Feminino , Sementes/citologia , Sementes/enzimologia , Rinite Alérgica Sazonal/diagnóstico , Rinite Alérgica Sazonal/metabolismo , Dieta/métodos , Urticária/patologia , Sementes/metabolismo , Rinite Alérgica Sazonal/complicações , Dieta , Urticária/prevenção & controleRESUMO
BACKGROUND: The relevance of contact allergy to plant-related food has recently emerged. Oral allergy syndrome is one of the most characteristic symptoms of fruit allergy, although it also causes systemic reactions. Plant-food allergy is increasing at the same time as pollen allergy, and fruit-induced allergic contact urticaria could be rising as well. OBJECTIVES: The present study was carried out in order to investigate whether one particular primary melon-peel allergen is responsible for contact urticaria. METHODS: Fourteen patients presenting with contact urticaria after touching melon peel were evaluated. A melon-peel extract was prepared and analysed by immunoblotting using the patients' sera. Molecular characterization of IgE-binding bands was performed using mass spectrometry. Melon-peel lipid transfer protein (LTP) was purified. Inhibition studies and contact challenge with the protein were performed to confirm IgE reactivity to the purified allergen. RESULTS: An IgE-binding band of ~8-9 kDa was observed in an immunoblotting assay with all the patients' sera and was identified as an LTP. The melon-peel LTP was purified in two chromatography steps. Inhibition studies confirmed LTP as a major allergen in patients with melon-peel contact urticaria. Contact challenge with melon-peel LTP was performed in five patients, all of whom had positive results, exhibiting itchy erythema and hives in the area of contact. CONCLUSIONS: This study confirmed our previous findings that melon-peel LTP is a major allergen and is responsible for contact allergy. This knowledge may be used to improve both diagnosis and treatment of patients allergic to melon.
