Lipid peroxidation in the liver and lungs in SiO(2)-induced granulomatosis.

LPO activity in the liver and lungs of Wistar rats during the development of granulomatous inflammation caused by intravenous injection of SiO(2)microparticles was evaluated by the content of conjugated dienes, ketodienes, conjugated trienes, and products of interaction of LPO intermediates with TBA. In the lungs, changes in LPO activity manifested in increased content of ketodienes and conjugated trienes (by 1.6 and 1.5 times on days 3 and 14 after injection of SiO(2)microparticles, respectively) and conjugated dienes (by 1.2 times on day 21) compared to the control levels, but the content of TBA-reactive substances remained within the normal range. In the liver, the content of conjugated dienes increased by 1.6 times and that of ketodienes and conjugated trienes by 2.3 times; the content of TBA-reactive substances increased by 1.7 times compared to the control. The content of TBA-reactive substances in the liver gradually increased and by day 14 this parameter surpassed the control level by 3.9 times, but on day 21 it returned to normal. Thus, LPO processes in the liver after injection of SiO(2)microparticles were more intensive than in the lungs and their activity underwent phasic changes.

Effect of lysosomotropic form of amphotericin B on functional state of phagocytes in experimental candidiasis.

Effect of antifungal preparation amphotericin B and its lysosomotropic composition with dialdehyde-dextran on functional state of phagocytizing cells in the dynamics of granulomatous inflammation induced by C. albicans was studied on CBA mice. A stimulating effect of amphotericin B on the production of reactive oxygen species by peritoneal and bone marrow phagocytes was observed, while lysosomotropic form of the antibiotic did not stimulate generation of oxygen radicals.

Effect of oxidixed dextrans on oxidative and metabolic function of mouse peritoneal macrophages in vitro and in vivo.

We compared the effects of dextrans with a molecular weight of 35 kDa oxidized by chemical (OD(ch)) and radiochemical (OD(r)) methods on oxidative and metabolic functions of peritoneal macrophages from BALB/c mice in vitro and in vivo. It was found that none type of dextrans exhibits chemiluminescent properties. In vitro study showed that OD(ch) had a priming effect on mouse peritoneal macrophages, while OD(r) did not potentiate the oxidative and metabolic response of cells to zymosan. Being injected intraperitoneally, OD(r) more markedly enhanced chemiluminescent response of mouse peritoneal macrophages and reduced their viability than OD(ch). Thus, both dextrans are biocompatible, but in OD(ch) this parameter is higher.