Effects of Inhaled Fluticasone Propionate on Wound Healing After Surgical Phonotrauma Model in Rabbit Larynx.

OBJECTIVES: The aim of this study was to evaluate the effects of inhaled fluticasone propionate on wound healing after phonosurgical trauma of larynx in rabbit model. STUDY DESIGN: A randomized controlled study on experimental animals (rabbits). METHODS: In this prospective experimental animal study, surgically induced type 2 scar was created under general anesthesia in 52 vocal folds of 26 rabbits. Inhaled fluticasone propionate was administered to 13 rabbits in the treatment group for 5 days after the trauma. Rabbits were sacrificed on the 10th, 30th, and 90th days. Histopathological examinations were performed to evaluate epithelization process, inflammation density, and collagen density at the wound side and the results compared between the groups. RESULTS: On the 10th day after surgical trauma, re-epithelialization was completed in both the treatment and the control groups. There was no difference between the groups in terms of mononuclear cell density on the 10th and 90th days (P > 0.05), but the inflammatory cell density was found to be lower in the treatment group on the 30th day (P = 0.005). Collagen density was significantly lower in all animals treated with inhaled fluticasone propionate, and sacrificed on the 10th, 30th, and 90th days, compared to the control group (P = 0.010, P = 0.038, and P < 0.001, respectively). CONCLUSIONS: Inhaled fluticasone propionate to be applied after phonotrauma reduces inflammation and collagen density in scar tissue in rabbits. Future clinical studies will be promising for the positive effects of inhaled steroids on voice quality after phonosurgery.

The danger of hyperoxia on the rat kidneys: is tadalafil a real shield?

PURPOSE: Continuous oxygen therapy to compensate for decreased oxygen saturation in the blood is a life-saving treatment used in case lung involvement. Excess oxygen delivery was reported to be a common situation, in which about 50% of the patients showed hyperoxemia and 4% in severe hyperoxemia. In this work, we investigated the effects of hyperoxia on the rat kidneys and whether tadalafil has an effect to reduce this damage. MATERIALS AND METHODS: Three groups of 8 male rats each weighing 300-350 g were formed. The groups were divided into the control group, hyperoxia group, and hyperoxia and tadalafil administered group for 10 days. At the end of the 10th day, blood and kidney samples were taken for biochemical analysis (SOD and NO levels) and histopathological examination. RESULTS: While our findings showed that SOD levels were significantly different among the control and experimental groups and within the experimental groups, no statistical difference was found in terms of NO levels among the groups (Table 1). While the glomerular and tubular injury was higher in the Hyperoxia group and the Hyperoxia + Tadalafil group than in the control group (p < 0.001), as a result of the rate of severe glomerular and tubular injury in the hyperoxia group, was 62.5% and 43.8% and in the group given tadalafil was 43.8% and 31.3%, respectively (Table 2). CONCLUSIONS: Exposure to hyperoxia condition causes renal glomerular and tubular damage, and tadalafil does not show a protective effect on this damage according to this study's dose and exposure time.

Tadalafil against hyperoxia-induced oxidative stress; an experimental study.

This study aimed to investigate the protective effect of tadalafil on reactive oxygen species induced by a hyperoxia model in rats, both in terms of enzymes such as superoxide dismutase (SOD) and nitric oxide (NO), and its pathological effects on the corpus cavernosum. Overall, 24 rats were divided into three groups. The control group (eight rats) was not exposed to any intervention. The second group (eight rats), was exposed to hyperoxia in a hyperoxia cabinet for 8 h a day for 10 days. The third group (eight rats) was exposed to hyperoxia the same as in the second group, tadalafil at a dose of 10 mg/kg was given orally as a dissolved form in water in the amount of 10-12 ml/100 g/day to the rats placed in separate cages having removed from the hyperoxia cabin. SOD levels differ enough to create a difference, but there was no significant difference in terms of NO levels. The SOD level was highest in hyperoxia conditions and lowest in the group given tadalafil. While corpus cavernosum hyperemia was found to be higher statistically in the experimental groups than in the control group, we found that the severity of hyperemia was less in the group given tadalafil. The corpus cavernosum was found to be statistically more dilated in the experimental groups than in the control group. We determined that hyperoxia status increased the level of SOD and this level decreased with tadalafil administration, which would make a statistical difference.