RESUMO
We report a two-photon fluorescent probe for ratiometric imaging of cysteamine in situ. This probe can detect the levels of endogenous cysteamine with statistical significance in live cells and brain hippocampal tissues, revealing that cysteamine is localized mainly in the perikaria of the pyramidal neurons and the granule cells.
Assuntos
Cisteamina/química , Corantes Fluorescentes/química , Animais , Linhagem Celular Tumoral , Cristalografia por Raios X , Cisteamina/metabolismo , Hipocampo/metabolismo , Humanos , Microscopia de Fluorescência , Fótons , RatosRESUMO
Mitochondria trafficking plays an essential role for supplying energy in the neuronal system. We report here a red emissive two-photon probe for mitochondria (CMT-red) that showed high selectivity and robust staining ability for mitochondria, high photostability under a two-photon microscopy imaging condition, and low cytotoxicity. This probe can be easily loaded into live cells and tissue and used for real-time, high resolution imaging of the mitochondria trafficking in primary cortical neurons as well as in rat hippocampal tissue.