Norfloxacin adsorption by torrefied coco peat biochar as a novel adsorbent in a circular economy framework.

The current study reported torrefied coco-peat biochar treated at 200 °C, as a novel adsorbent exhibiting phenomenal norfloxacin (NFX) adsorption efficiency. The CHNS analysis confirmed the carbon abundance in the biochar (36.45%), however, XRF analysis indicated a significant presence of K2O (27.73%) and chlorine (7.49%). The XRD and Raman spectral analysis confirmed the amorphous structure of the biochar. Multilayer topology was evident in the SEM micrograph of biochar contributing to its large effective surface area. Additionally, the mesoporous structure of the adsorbent was verified by BET. The adsorption mechanism was predicted to be non-ionic since the zeta potential of both adsorbent and adsorbate was found negative. The process parameters were optimized at 30 °C, pH 6.9, dosage 7 g/L, antibiotic load 494.25 mg/L, and time of 89 min for a maximum of 99.52% adsorption of NFX using Central Composite Design, Analysis of Variance, and Response Surface Methodology. The adsorption process was exothermic, and spontaneous obeying the pseudo-second-order kinetics, while the bulk process was confined to surface adsorption. Isotherm study of NFX adsorption revealed the process to be a favorable, monolayer, and homogeneous adsorption. The NFX molecules were desorbed with an efficiency of 89.19% using 80% ethanol and upon recrystallization, 87.76% of the initial NFX was recovered as crude crystal. Moreover, the NFX removal efficiency was consistent across various water systems, tap water (99.02%), seawater (99.56%), river water (98.92%), pond water (98.26%), and distilled water (99.17%). The techno-economic analysis identified bulk expense as the biochar preparation ($0.82/kg) and the process will be profitable having recovered NFX sold at $6/kg instead of the present retail price ($71/kg). Thus, the study successfully demonstrated a zero-waste, self-sustainable, and revenue-generating water treatment process implementing the circular economy framework.

Biotransformation of diclofenac by isolated super-degrader Pseudomonas sp. DCα4: Postulated pathways, and attenuated ecotoxicological effects.

Significant concentrations of emerging xenobiotics, like diclofenac (DCF), possessing severe irreversible eco-toxicological threats, has been detected in aquatic systems worldwide, raising the concerns. This present investigation is intended to explore an efficient solution to support the existing wastewater treatment policies to handle DCF contamination by bacteria-mediated biotransformation. DCF-tolerant bacterial strains were isolated from pharmaceutical wastewater and selected based on their non-virulence nature and degradation ability. Among those, Pseudomonas sp. DCα4 was found to be the most dominant DCF degrader exhibiting 99.82% removal of DCF confirmed by HPLC after optimization of temperature at 30.02 °C, pH at 6.9, inoculum of 4.94%, and time 68.02 h. The degradation kinetics exhibited the process of DCF degradation followed a first-order kinetics with k of 0.108/h and specific degradation rate of 0.013/h. Moreover, the enzyme activity study indicated predominant hydrolase activity in the DCF treatment broth of DCα4, implying hydrolysis as the main force behind DCF biotransformation. HRMS analysis confirmed the presence of 2-hydroxyphenylacetic acid, 1,3-dichloro,2-amino, 5-hydroxybenzene, and benzylacetic acid as major intermediates of DCF biodegradation indicating non-specific hydrolysis of DCF. Whole genome analysis of most related strains which were confirmed by near full 16S rRNA gene sequence homology study, predicted involvement of different N-C bond hydrolase producing genes like puud, atzF, astB, nit1, and nylB. The ecotoxicological study using Aliivibrio fischeri exhibited 47.51% bioluminescence inhibition by DCF-containing broth which was comparable to the same caused by 1 mg/mL of K2Cr2O7 whereas remediated broth exhibited only 0.51% inhibition implying reduction of the ecotoxic load caused by DCF contamination. Cost analysis revealed that possible integration of the process with existing ones would increase per litre expense by $0.45. These results indicated that the described process of DCF biodegradation using the super-degrader DCα4 would be an advancement of existing pharmaceutical wastewater treatment processes for DCF bioremediation.

Mitigation of hydrocarbon toxicity using bacterial consortium in microcosm environment for agrarian fecundity.

Petroleum contamination in the soil has been well emphasized as a toxic and hazardous soil pollution contributing to a significant portion of soil infertility worldwide. In the present study, bacterial consortium CHM1 composed of 5 strains belonging to genera Klebsiella, Pantoea, and Enterobacter was evaluated for hydrocarbon degradation ability in the soil environment, as well as their performance in remediating ecotoxicity and phytotoxicity. Initially, the degradation efficiency (1.98%/day) in the soil environment was evaluated. Scanning Electron Microscopy combined with Energy Dispersive X-ray spectroscopy revealed an increase in nitrogen content by 24.98% and a decrease in carbon content by 22.76% implying an improvement in soil fertility. The Fourier Transform InfraRed spectroscopy and Gas Chromatographic analysis revealed significant depletion of aromatic, cyclic, long aliphatic, and complex acid and ester content of the test soil. Moreover, the quantitative PCR analysis exhibited the non-competitive coexistence of each component of the CHM1 consortium. Different enzymatic assays revealed elevated dehydrogenase and superoxide dismutase activity in the degradation system due to the introduction of CHM1 in the soil microcosm. Vibrio fischeri-assisted ecotoxicity analysis had established the potential of CHM1 to efficiently minimize the ecotoxicity of hydrocarbon contamination. The phytotoxicity analysis was performed using four different plant models viz. Chickpeas (Cicer arientinum), Coriander (Coriandrum sativum), Fenugreek (Trigonella foenum-graecum), and Spinach (Spinacia oleracea) exhibiting CHM1 amendment helped to restore plant germination and growth in hydrocarbon-contaminated soil system efficiently. The promising results from this study indicated the possible application of the bacterial consortium in hydrocarbon-contaminated land management and soil restoration for cultivation or other plantation purposes.

Techno-economic assessment of doxycycline recovery using rice straw biochar: A circular economic execution.

The non-scientific disposal of antibiotics has resulted in massive contamination of the bioactive molecules in the aquatic ecosystem. The presence of antibiotics in the effluents limits the biodegradation of micropollutants by affecting the micro-ecological balance. Hence this study aims to remove doxycycline antibiotics from wastewater using biochar. Elemental analysis of the biochar revealed C, Si and N as most abundant content while BET analysis confirmed the mesoporous nature of the adsorbent. The XRD and Raman spectra confirmed amorphic sp2 carbon dominant structure in the biochar. The adsorption mechanism was predicted, correlating the charge distribution and FTIR analysis. The effects of different process parameters were studied using CCD, ANOVA, and RSM. Moreover, the different kinetic models revealed that the pseudo-second-order kinetics model was the best fit and film layer diffusion was the dominant contributor. The isotherm study indicated the high adsorption capacity of the biochar and its non-ionic nature. Thermodynamics study established the spontaneity and exothermic nature. The results suggested no significant change in antibiotic removal efficiency across different system (pond water (97.13%), river water (98.11%), seawater (96.84%), tap water (99.13%), and distilled water (99.74%)). For the desorption of the antibiotic from the biochar surface, 90% ethanol was the most efficient (98.9%), and upon recrystallization by solvent evaporation, 98.7% of the antibiotic of the initial load was recovered. Hence, the implementation of this described process would enable resource recovery along with water treatment, which is not possible with existing approaches. The cost analysis of the whole process revealed that biochar preparation was the bulk expense and the process would be self-sustainable even if the price of the recovered antibiotic would be set at less than half ($41/kg) of the current market price ($94/kg) of the API. Thus, the process endorses a successful circular economy approach toward societal and economic sustainability.

Construction of bacterial consortium for efficient degradation of mixed pharmaceutical dyes.

Synthetic dyes are established colorants in the pharmaceutical industries for the coating and coloration of tablets, capsules, etc. to mark the specific formulation and dosage, improve the stability, and esthetic value. The pharmaceuticals manufacturing process releases large volumes of dye-containing wastewaters, contributing to eco-toxic concerns related to different health risks. In this study, biodegradation of amaranth, sunset yellow, tartrazine, indigo carmine, and quinoline yellow dyes was investigated in the mixed environment. Initially, 24 tolerant bacterial strains were isolated from pharmaceutical wastewater samples, among which 19 strains were found to be non-virulent. Five different consortia were constructed by considering the 19 strains among which C1 consisting of strains DY7, 10, 11, and 18 was found to be the most potential consortium with an overall efficiency of 96.65% dye degradation in the presence of glucose after 15 days. The strains DY7, 10, 11, and 18 were identified as members of the Klebsiella genus by 16S rRNA partial sequencing. The cell wall structure, carbohydrate utilization profile, and metabolic characterization were conducted on the selected strains of C1. Among the 25 different antibiotics, DY7 and DY11 exhibited the highest zone of growth inhibition in the presence of cefixime, and DY10 was restricted by chloramphenicol whereas DY18 was found sensitive to ceftazidime/tazobactam.

Biofilm-Mediated Heavy Metal Removal from Aqueous System by Multi-Metal-Resistant Bacterial Strain Bacillus sp. GH-s29.

Worldwide ever-augmenting urbanization, modernization, and industrialization have contributed to the release of pernicious compounds and a variety of pollutants into the environment. The pollutants discharged due to industrialization are of global concern. Industrial waste and effluent are comprised of hazardous organic and inorganic chemicals including heavy metals which pose a significant threat to the environment and may bring about numerous diseases or abnormalities in human beings. This brings on greater urgency for remediation of these polluted soil and water using sustainable approaches and mechanisms. In the present research, a multi-metal-resistant, gram-positive, non-virulent bacterial strain Bacillus sp. GH-s29 was isolated from contaminated groundwater of Bhojpur district, Bihar, India. The strain had the potential to develop a biofilm that was able to remediate different heavy metals [arsenic, cadmium, and chromium] from individual and multi-heavy metal solutions. Maximum removal for As (V), Cd (II), and Cr (VI) from individual-metal and the multi-metal solution was observed to be 73.65%, 57.37%, 61.62%, and 48.92%, 28.7%, and 35.46%, respectively. SEM-EDX analysis revealed the sequestration of multi-heavy metals by bacterial biofilm. Further characterization by FTIR analysis ensured that the presence of negatively charged functional groups on the biofilm-EPS such as hydroxyl, phosphate, sulfate, and carboxyl helps in binding to the positively charged metal ions. Thus, Bacillus sp. GH-s29 proved to be an effective and economical alternative for different heavy metal remediation from contaminated sites.

Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19.

A pink pigment-producing bacterial strain was isolated from wastewater and identified as Paenibacillus sp. BPW19. The motile bacterial strain was Gram-positive, acid fermenting, glucose, sucrose utilizing and rod-shaped with an average cell length of 1.55 µm as studied under the Environmental Scanning Electron Microscope. Even though being psychrotolerant, the cell growth condition of BPW19 was optimized as 25 ºC along with pH 8, and 2.25% inoculum concentration considering the operational ease of the production. Sonication assisted solvent extraction produced 5.41% crude pigment which showed zones of exclusion against gram-negative strains Escherichia coli DH5α, Enterobacter sp. EtK3, and Klebsiella sp. SHC1. Gas Chromatography-Mass Spectrometry analysis of the crude pigment exhibited the dominant presence of major compounds as dotriacontane; 3,7 dimethyl 7 octanal; 1-eicosene and erucic acid. While column chromatography (ethanol:chloroform in 1:4 (v/v) ratio) purified pigment was identified as erucic acid using Nuclear Magnetic Resonance with a net yield of 3.06%.

Fruit waste management by pigment production and utilization of residual as bioadsorbent.

Dry powder of fruit wastes including pomegranate, grapes, lime, apple, and papaya was used to examine their pigment extraction capability. Solvent combination of hexane and acetone (1:1) produced the maximum extraction for lime waste with a yield of 1.65%. Gas Chromatography and Mass Spectrometry analysis of the crude pigment of lime exhibited the abundance of compounds like chlorogenic acid, caffeic acid, and coumaric acid. Three major bands were obtained in Thin Layer Chromatography. Column Chromatographic purification using ethyl acetate and hexane mixture as eluting phase showed a retention factor of 0.62 for the major band. Nuclear Magnetic Resonance revealed the final structure of the compound as chlorogenic acid. Slight inhibition was exhibited by the compound against Escherichia coli. Adsorption of arsenate by residual fruit waste revealed the highest adsorption in grapes with 92% efficiency. This study enlightens an alternative approach of fruit waste management with the production of value-added products and its utilization of residue as bioadsorbent.