Antioxidant and Antivenom Potential of an Essential Oil, 4-(2-Oxo-propyl)-cyclopentane-1,3-dione, and Allantoin Derived from the Polyherbal Combination of Aristolochia indica L. and Piper nigrum L.

The goal of this study was to identify new compounds from a methanol extract of a polyherbal combination of Aristolochia indica L. and Piper nigrum L. (MECAIPN), two traditional medicinal plants used to cure envenomation, as well as to assess their antioxidant and antivenom properties. MECAIPN yielded EA1 (an essential oil), AA2 (4-(2-oxo-propyl)-cyclopentane-1,3-dione), and W3 ((2,5-dioxo-imidazolidin-4-yl)-urea) (Allantoin). Although EA1 had stronger radical scavenging activity, AA2 had higher DPPH and ferric ion radical scavenging activity, and W3 had higher molybdenum ion radical scavenging activity due to being a single molecule, the binding investigation revealed that EA1 has a greater Stern-Volmer quenching constant (Ksv) than AA2 and W3. Synchronous measurements indicated that EA1, AA2, and W3 bind to tryptophan and tyrosine residues in venom, causing denaturation of the secondary structure of the residue. Finally, the current study concludes that EA1 has more therapeutic antivenom potential, which could be related to the synergism of chemicals found in it. When it came to single compounds, AA2 had stronger antioxidant and antivenom capabilities than W3. To understand the mechanism of action and manufacture the green antivenom medication, more testing of the EA1 and compounds remains required.

A concise review of mushrooms antiviral and immunomodulatory properties that may combat against COVID-19.

The World Health Organization (WHO) declared COVID-19 as a pandemic on March 11, 2020, because of its widespread transmission and infection rates. The unique severe disease was found in Wuhan, China, since December 2019, and swiftly spread throughout the world. Natural chemicals derived from herbal medicines and medicinal mushrooms provide a significant resource for the development of novel antiviral drugs. Many natural drugs have been proven to have antiviral properties against a variety of virus strains, such as the coronavirus and the herpes simplex virus (HSV).. In this research, successful dietary treatments for different COVID illnesses were compared to potential of mushroom products in its therapy. In Google Scholar, Science Direct, PubMed, and Scopus, search keywords like COVID, COVID-19, SARS, MERS, mushrooms, and their compounds were utilized. In this review of the literature we foucsed popular mushrooms such as Agaricus subrufescens Peck, Agaricus blazei Murill, Cordyceps sinensis (Berk.) Sacc., Ganoderma lucidum (Curtis.) P. Karst., Grifola frondosa (Dicks.) Gray, Hericium erinaceus (Bull.) Pers., Inonotus obliquus (Arch. Ex Pers.) Pilát., Lentinula edodes (Berk.) Pegler, Pleurotus ostreatus (Jacq.) P. Kumm., Poria cocos F.A. Wolf, and Trametes versicolor (L.) Lloyd.,. Changed forms of ß-Glucan seem to have a good impact on viral replication suppression and might be used in future studies. However, the results seems terpenoids, lectins, glycoproteins, lentinan, galactomannan, and polysaccharides from mushrooms are promising prophylactic or therapeutic agents against COVID-19.

Evaluation of Aristolochia indica L. and Piper nigrum L. methanol extract against centipede Scolopendra moristans L. using Wistar albino rats and screening of bioactive compounds by high pressure liquid chromatography: a polyherbal formulation.

The present study was aimed to explore the anti-venom activity of Aristolochia indica and Piper nigrum plants against the centipede (Scolopendra moristans) envenomation in animal model. In vtiro phytochemical, antioxidant and blocking of proteolysis were carried out by using standard spectrophotometric methods. In vivo anti-venom activity of methanol extracts was determined using Wistar albino rats after fixing lethal and effective doses. The electrolytes, lipid, liver, kidney, hematological parameters were analyzed and histopathology of skin and liver were also examined. Anti-skin cancer by MTT method and HPLC analysis were also carried out. The CAIPN extract showed higher total phenolics (150.65 ± 0.08 mg GAE/g extract) and flavonoids (158.97 ± 0.93 mg RE/g extract) content. Further, the same extract revealed the higher molybdenum reducing, inhibition of lipid peroxidation (80.08 ± 0.22%), DPPH radical scavenging (3.05 µg/mL), and blocking of proteolysis activities (96.45 ± 0.04%). The parameters like hypersensitivity, electrolytes, lipids, blood components, liver and kidney marker of the CAIPN methanol extract (200 mg/kg) treated envenomated rats was remarkable and same as in the normal animals. Such status was also achieved by RBAI and SPN at 600 mg/kg. The histopathological scoring of skin and liver confirmed the venom neutralizing activity of CAIPN. Also, the CAIPN methanol extract was notable in anti-skin cancer activity (208 µg/mL). The presence of the ferulic acid (04 ± 0.09 µg/mg) and quercetin (35.30 ± 0.30 µg/mg) like compounds was confirmed by HPLC analysis. Hence, the present investigation results conclude that the CAIPN was significant in their action and this polyherbal formulation could be considered as a new source for the pharmaceutical industries to develop a new effective, ecofriendly anti-venom drug.

Chemical profiling of Pterolobium hexapetalum leaves by HPLC analysis and its productive wound healing activities in rats.

Research on wound healing agents is one of the developing areas in modern biomedical sciences. Several drugs have been used in the management of wounds and a large variety of treatment modalities are available for wound repair. Among these medicines, herbal origin has been the basis of treatment and cure for healing of wounds. Pterolobium hexapetalum is well known medicinal plant of Caesalpiniaceae family was used by many tribal groups for wound treatment in India. Different extracts of P. hexapetalum leaves are evaluated for its antiradical activity and in vivo wound healing activity by excision, incision, and dead space wound models in Wistar rats, further respective phyto constituents are profiled using High Performance Liquid Chromatography. In this present study methanol extract of leaves depicted the maximum amount of phenolics and flavonoids content and also registered highest DPPH (IC50: 3.40µg/mL), ABTS+(8869.36µM TE/g extract) and Superoxide (87.72%) radical scavenging activity. Methanolic leaf extract are further studied for wound healing property, highest wound contraction percentage and lowest epithelialization period (16.33days) of 5% methanolic extract treated group exhibits highest wound healing activity compare to control group and it was confirmed by histopathology results and scoring analysis. Maximum tensile strain (110.69%) of incision and highest hydroxyproline (16.28mg/g) content of dead space wound model are comparable with standard 5% Neomycin. HPLC employed quantification of phyto constituents explores the presence of catechin (367.54µg/mg), caffeic acid (87µg/mg), rutin (289.20µg/mg), quercetin (239.39µg/mg) and naringenin (24.42µg/mg). From the results leaves of P. hexapetalum significantly stimulate wound contraction, braking strength and hydroxyproline content of treated group. It showed remarkable wound healing activity and may be recommended for treating various type of wound.