RESUMO
Porous polymeric scaffolds are extensively studied for delivery of bone growth factors. Since phytochemicals are known to produce changes in cell signalling and other metabolic pathways, osteogenic phytochemicals, that is, extracts of Cissus quadrangularis and Butea monosperma, are incorporated into sulphonated poly(aryl ether ketone) sponges. The results have shown that the scaffolds with phytochemicals enhanced the proliferation and alkaline phosphatase activity of the cells compared to cells treated on scaffolds without phytochemicals. Hence, these phytochemicals can be evaluated to augment, if not substitute the use of bone morphogenetic proteins in scaffolds.
Assuntos
Osso e Ossos , Compostos Fitoquímicos/farmacologia , Engenharia Tecidual/instrumentação , Alicerces Teciduais/química , Fosfatase Alcalina/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Antraquinonas/química , Butea/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cissus/química , Amarelo de Eosina-(YS)/química , Humanos , Oxazinas/química , Porosidade , Xantenos/químicaRESUMO
BACKGROUND: In human anatomy, a nail is a hornlike envelope covering the dorsal aspect of the terminal phalanges of fingers and toes. Nail disorders are most common among the geriatric population. Diabetes mellitus is also supposed to affect the condition of nails. Acceptable differences in infrared (IR) spectra of chronic and acute diabetes mellitus patient fingernail specimens compared to control normal specimens were investigated in this study. METHODS: Using a Nicolet 360 Fourier Transform Infra Red (FTIR) spectrometer, the spectra of the nails of diabetics and normal specimens were recorded. RESULT: In the case of non-diabetic patients, the amide I band was observed <1640 cm(-1) (1626, 1632, and 1638 cm(-1)). The bands around 1637 cm(-1), were attributable to amide I of ß sheet structures. Amide II bands were absent in all the non-diabetic patients. Amide III bands around 1250 cm(-1) were observed both in diabetic and non-diabetic patients. In all the diabetic patients, a peak of <500 cm(-1), particularly around 468 cm(-1), was observed. CONCLUSION: The proteins in the nails of diabetic patients contain α-helical structure, including the presence of amide II bonds. Alkyl thiolated structures are observed. Nails of non-diabetic patients do not have the amide II structures.
