RESUMO
Buffalopox virus (BPXV) is considered to be a close variant of vaccinia virus (VACV), the prototype member of the genus Orthopoxvirus. In the present study, we have analyzed the sequences of H3L, A27L, and D8L gene-homologues of VACV in BPXV to elucidate its genetic relationship to VACV and other orthopoxviruses (OPVs). Products of these genes have been shown to be important in attachment of VACV to host cell surface receptors during viral entry. Additionally, the A27L gene is also responsible for cell fusion during infection, while the H3L gene is required for synthesis of the highly immunogenic major envelope protein p35. Full-length nucleotide sequences of H3L, A27L, and D8L genes of three BPXV isolates were determined by PCR amplification, cloning, and sequencing. The nucleotide (nt) sequence and the deduced amino acid (aa) sequences were compared with published sequences from other members of the genus Orthopoxvirus. Comparative sequence analysis of all the three genes revealed high sequence identity of BPXV isolates with VACV (close to 99% sequence identity) at both the nt and aa level. Phylogenetic analysis based on the deduced aa sequences of the H3L, A27L, and D8L genes also showed that BPXVs are more closely related to VACV than to any of the other OPVs.
Assuntos
Genes Virais , Vaccinia virus/genética , Proteínas do Envelope Viral/genética , Sequência de Aminoácidos , Fusão Celular , Clonagem Molecular , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de Proteína , Homologia de Sequência , Especificidade da Espécie , Vaccinia virus/classificação , Vaccinia virus/fisiologia , Proteínas do Envelope Viral/fisiologia , Replicação ViralRESUMO
An outbreak of buffalopox in domestic buffaloes, with high morbidity and significant production loss, was recorded in the Aurangabad district of Maharashtra State in India in November 2003. The disease was also associated with several cases of human infection, particularly in milkers working with the affected herds. Pox lesions were observed on the udder and teats of the majority of the affected animals, while a few animals exhibited lesions on the hindquarters, indicating possible generalised infection. A significant reduction in milk yield was recorded following the outbreak. Milkers developed pox-like lesions on the skin of the hands, forearms and forehead accompanied by fever for three days, axillary lymphadenopathy and general malaise. Investigation of the disease outbreak by virus isolation in Vero cell cultures and detection of viral nucleotide sequences by polymerase chain reaction (PCR) confirmed the aetiology of the disease.
