Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon.

Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.

Involvement of P2Y receptors in the protective effect of ATP towards the cell damage in HaCaT cells exposed to H2O2.

It has recently been reported that activation of P2Y(1) receptor, one of the purine receptors, by extracellular nucleotides induces cytoprotection against oxidative stress. In this study, we examined the protective effect of ATP on the cell damage in human epidermal keratinocyte HaCaT cells exposed to H(2)O(2) via the P2Y receptor-mediated induction of intracellular antioxidants. The cells were damaged by exposure to H(2)O(2) in a dose- and time-dependent manner. The damage induced by 7.5 mM H(2)O(2) was blocked by pretreatment of the cells with ATP (1-10 µM). The protective effect of ATP was significantly reduced by P2Y receptor antagonists. Exogenously added ATP induced various intracellular antioxidants, including thiol-containing proteins, Cu/Zn superoxide dismutase (SOD) and thioredoxin-1, in HaCaT cells. In conclusion, it was found that ATP protected the cells from the H(2)O(2)-induced cell damages via the P2Y receptor-mediated induction of intracellular antioxidants.

Efficacy of Corrected Rapid Turnover Protein Increment Index (CRII) for Early Detection of Improvement of Nutrition Status in Patients with Malnutrition.

Serum prealbumin level is useful for assessment of changes in nutritional status but it is markedly affected by the inflammation. In this study, we examined the efficacy of the corrected rapid turnover protein increment index (CRII) for prealbumin, which is calculated as [prealbumin level/C-reactive protein (CRP) level on the assessment day]/[prealbumin level/CRP level on the day of starting nutritional care], for prediction of improvement of nutritional status in patients with malnutrition. The subjects were 50 hospitalized patients with low albuminemia, who were receiving nutritional care. Serum concentrations of albumin, prealbumin and CRP were measured every week for 5 weeks. We defined patients whose serum albumin level was elevated by more than 0.2 g/dl after 5 weeks as those showing improved nutritional status. There was a significant difference in the prealbumin level between improved and unimproved patients at 5 weeks after the start of nutritional support. On the other hand, the prealbumin CRII value showed a significant difference between the groups at 1 and 2 weeks after the start of nutritional support. In conclusion, assessment of prealbumin CRII is useful for early prediction of improved nutritional status in patients with malnutrition.

Effects of overtime work on blood pressure and body mass index in Japanese male workers.

BACKGROUND: Effects of overtime work on health is a controversial issue. AIMS: To determine the effects of overtime work on blood pressure and body mass index (BMI) for male workers in Japan. METHODS: Participants were 323 male participants of three companies. Data were collected by occupational physicians at periodic physical examinations and additional examinations. The time courses for the development of definite hypertension and an increase in BMI were recorded. The Kaplan-Meier method was used to estimate the cumulative incidence rates of developing definite hypertension and increasing BMI, and the Cox proportional hazard model was used to determine the adjusted relative hazard of overtime work. RESULTS: The age-adjusted incidence rates of developing definite hypertension and increasing BMI were significantly lower among the participants whose mean overtime was > or =50 h than among those whose mean overtime was <50 h/month (log-rank P < 0.05). The Cox proportional hazard model indicated that those who worked a mean overtime of > or =50 h/month had lower risks of developing definite hypertension (hazard ratio, 0.36; 95% CI, 0.15-0.88; P < 0.05) and increasing their BMI (hazard ratio, 0.44; 95% CI, 0.31-0.63; P < 0.01) after adjusting for age. CONCLUSIONS: The results of this study indicate that workers whose mean overtime was > or =50 h have lower risks of developing definite hypertension and increasing their BMI.

Somatoform disorders in the workplace in Japan.

The literature related to somatoform disorders in the workplace is very limited, and these disorders need more attention from mental health professionals in the workplace as well as from employers. Over the last decade, major changes have taken place in the work environment in Japan. More stress and less support from supervisors or colleagues in the workplace have made employees stressed out. The number of employees with mental disorders, including somatoform disorders, taking sick leave has significantly increased. In our multi-centre collaborative study, somatoform disorders were the third most prevalent psychiatric disorder in employees, after mood and schizophrenic disorders. Employees with neurotic disorders manifested physical symptoms more frequently than those without. Young employees frequently reported somatic symptoms such as general malaise, nausea, constipation, diarrhoea, headache, stiff shoulder, and dizziness. A rational new approach is needed to tackle this important psychopathology increasingly seen among employees.