Welfare Assessment on Different-Sized Dairy Goat Farms in the Northern Serbian Province of Vojvodina.

This research aimed for the first time to identify the essential welfare problems on 46 goat dairy farms in Vojvodina,based on the goat AWIN welfare protocol. The study showed that space allowance per goat was 1.55, 1.86 and 2.50 m2/goat (large, medium, small-sized farms, respectively). Most farms possessed the soil floor and straw as a flooring material. The small and medium farms more allowed access to the outdoor area. Contrary, large-sized farms mostly kept goats in fully housed systems with reduced opportunities for the goats to express their natural behavior. Management procedures were significantly different (p < 0.05) between the investigated farms. Differences were mainly in bedding, feeding and culling strategies. Low productivity was the main cause of culling in all farm types. Small-sized farms carried out more often disbudding (42.9%) in comparison with medium (21.4%) and large-sized ones (14.3%). Group level observation showed that the main areas of concern were associated with hair coat condition and kneeling at the feeding rack (p < 0.05). Inadequate BCS, udder asymmetry and claw overgrowth were frequently recorded. The prevalence of thin and fat goats was highest in large-sized farms All farm size categories and management systems showed certain welfare weaknesses. Thus, the findings highlighted the need for higher implementation of animal welfare principles and improvement of management techniques to meet the welfare requirements of dairy goats.

Transabdominal and transrectal ultrasonography of fetuses in Württemberg ewes: Correlation with gestational age.

It is useful to determine the gestational age in sheep to provide essential information for effective flock management practices. The aims of this study were to evaluate the efficacy and practical aspects for using two ultrasound techniques, transabdominal and transrectal, in determining the gestational age in Württemberg ewes. Monitoring of embryo and fetus developmental stages during the ewes' gestation was carried out with real time ultrasound using a transabdominal convex probe, frequency 3.5 MHz, and a transrectal linear probe, frequency 7.5 MHz. The size of the embryonic vesicle during the period from the 23rd to the 38th day of gestation can be used as a confirmational indicator of gestational age when the transrectal probe is used. The occipital nasal diameter correlated with the gestational age with both transabdominal (P < 0.05) and transrectal probes (P < 0.01) from the 46th to the 63rd day of gestation. The biparietal diameter of the fetal head measured by transabdominal probe during the period from the 46th to the 63rd day of gestation correlates with gestation age (P < 0.05). The diameter of the fetal eye orbit monitored by transrectal probe from the 46th to the 63rd day of gestation also correlated well with gestational age (P < 0.05).

Downregulation of nicotinic and muscarinic receptor function in rats after subchronic exposure to diazinon.

Diazinon (DZN) is an organophosphate insecticide which exerts its effect through the inhibition of acetylcholinesterase enzyme (AChE). In this work, we studied the development of tolerance to subchronic p.o. administration of DZN in rats, under both in vivo and in vitro conditions. A group of 20 rats (2 groups, n = 10) was administered p.o. the 1/10 of established LD50 DZN (namely 55.87 mg/kg bw) for 28 days. On the 14th and 28th day of study with isolated diaphragm and ileum, we examined the downregulation of nicotinic and muscarinic receptor function through Electrical Field Stimulation (EFS). Maximum contractility of the diaphragm was recorded on the 14th day of the study (25% higher compared to the non-treated rats), while on the 28th day the contractions almost did not differ from the values found in non-treated rats. EFS of isolated ileum on the 14th day of study caused significantly higher contractions compared to the non-treated rats, but after 28 days, ileum contractions decreased approximately to the level of contractions in non-treated rats. On the 14th study day, we also recorded increased amplitude of spontaneous ileum contractions, compared to non-treated rats. The application of increasing ACh concentrations caused dose-dependent ileum contractions, without statistically significant differences of median effective concentration (EC50) values in non-treated and treated rats. Tolerance to subchronic DZN administration develops due to various adaptation mechanisms, including the most important one-downregulation of nicotinic and muscarinic receptor function.