RESUMO
The kinetics of [3H]-L-glutamate binding to brain synaptic membranes (SM) and to glutamate-binding proteins (GBP) was determined with agonist and monoclonal antibodies (MAbs). It was revealed, that rat and human brain GBP have individual protein components with M(r) from 14 to 92 kDa. Quisqualate inhibited [3H]-L-glutamate binding to solubilized and to purified 68 kDa protein component. MAbs have the most activity, and NMDA was failure. It has been shown that 68 kDa component antigen determinants are similar to those of bovine, frog and rat brain synaptic membranes. Anti-GBP monoclonal antibodies blocked functional non-NMDA receptors in isolated frog spinal cord. Immunocytochemistry was done on rat and human brain sections. Distribution of quisqualate receptors was determined with light and electron microscopy. Some properties of vertebrate CNS non-NMDA receptors are discussed.
Assuntos
Encéfalo/efeitos dos fármacos , Ácido Quisquálico/metabolismo , Receptores de Glutamato/efeitos dos fármacos , Adulto , Animais , Anticorpos Monoclonais , Encéfalo/metabolismo , Encéfalo/ultraestrutura , Glutamatos/imunologia , Glutamatos/metabolismo , Humanos , Imuno-Histoquímica , Ligantes , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Microscopia Imunoeletrônica , Ligação Proteica/efeitos dos fármacos , Rana ridibunda , Ratos , Receptores de AMPA , Receptores de Glutamato/metabolismo , Receptores de Glutamato/ultraestruturaRESUMO
A simplified micromodification of the leukocyte adherence inhibition (LAI) test using both leukocytes and lymphocytes from peripheral blood of cancer patients is discussed. Data on oncoimmunologic specificity and cellular mechanisms underlying the proposed procedure as well as the results of a comparative study of LAI factor-producing T-cell and immunoregulatory (Tmu and T gamma) lymphocyte subpopulations in breast cancer patients are presented. The LAI-test micromodification may be adjunct in breast cancer diagnosis. It may be employed for monitoring T-cell specific immunity in cancer patients and further study of LAI factor-producing T-cells as a component of the structure of T-cell subpopulations.
Assuntos
Neoplasias da Mama/diagnóstico , Técnicas Imunológicas , Teste de Inibição de Aderência Leucocítica , Neoplasias da Mama/imunologia , Feminino , Humanos , Técnicas Imunológicas/instrumentação , Teste de Inibição de Aderência Leucocítica/instrumentação , Leucócitos/imunologia , Métodos , Proteínas de Neoplasias/análise , Receptores Fc/análise , Linfócitos T/imunologiaRESUMO
The simplified micromodification of the leukocyte adhesion inhibition (LAI) test has shown that the LAI tests both for lymphocytes and for leukocytes may be equally used as additional methods of diagnostics of the early stages of breast cancer. The detailed study of the kinetics and cellular mechanisms of the method has shown that the LAI reaction belongs to the earliest reactions of T-cellular immunity and is mediated by T-cell lymphokine. The study of sensitivity and specificity of the method revealed that the suggested micromodification of the LAI test may be used in experimental and clinical research for the study of the subpopulation of T-cells-producers of the LAI factor as well as for monitoring of the T-cell immunity in the cancer patients during treatment.
