Assuntos
Córtex Cerebral/patologia , Esquizofrenia/patologia , Adolescente , Adulto , Idoso , Astrócitos/ultraestrutura , Núcleo Celular/ultraestrutura , Retículo Endoplasmático/ultraestrutura , Feminino , Humanos , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Mitocôndrias/ultraestrutura , Neurônios/ultraestruturaAssuntos
Córtex Cerebral/ultraestrutura , Hipotálamo Anterior/ultraestrutura , Hipotálamo/ultraestrutura , Mudanças Depois da Morte , Animais , Autólise , Núcleo Celular/ultraestrutura , Retículo Endoplasmático/ultraestrutura , Complexo de Golgi/ultraestrutura , Humanos , Lisossomos/ultraestrutura , Mitocôndrias/ultraestrutura , Ratos , Fatores de TempoRESUMO
Among the neurons of the frontal pole of the brain cortex in rats in the norm there is a small, increasing with years, amount of "dark" cells, Regularities in their distribution in the cortical layers were not detected. In transition from the optical picture with the use of halfthin sections for the identification of the corresponding cells to an electronomicroscopic study of "dark" neurons it was found that these cells possess a certain type of ultrastructure: an increased number of ribosomes, deep invagination of karyolemms, an expansion of the perinuclear space, a significant number of slit, tortuose channels of the endoplasmatic reticulum, a somewhat increased laminar Golgi complex with large vacuoles. With the aid of autoradiography it was established that there is a drop in the level of the RNA synthesis in the nucleus and a drop in the velocity of the migration of newly formed RNA into the cytoplasma of "dark" cells.
Assuntos
Lobo Frontal/citologia , RNA/biossíntese , Animais , Autorradiografia , Núcleo Celular/metabolismo , Lobo Frontal/metabolismo , Lobo Frontal/ultraestrutura , Microscopia Eletrônica , RatosRESUMO
Using electron cytochemistry the authors studied the brain frontal cortex and hypothalamus in rats and humans in different periods following death. With the aid of 3,3'-diaminobenzidin, cytochrome C which was located inside the crystal and between the external and internal membrane of the mitochondria was detected. ATP-ase and acid phosphatase was revealed by the lead method. The activity of acid phosphotase was demonstrated in the nucleoli, along the membrane of the cytoplasmatical reticulum, in the symaptical terminals and in the circulatory capillaries. It was established that in the human brain it is possible to study the localization of the cytochrome C up to 24 hours, ATP-ase up to 15-20 hours and acid phosphatase up to 12 hours following death.
Assuntos
Lobo Frontal/metabolismo , Hipotálamo/metabolismo , Mudanças Depois da Morte , Núcleo Supraóptico/metabolismo , Fosfatase Ácida/metabolismo , Adenosina Trifosfatases/metabolismo , Animais , Grupo dos Citocromos c/metabolismo , Lobo Frontal/enzimologia , Lobo Frontal/ultraestrutura , Histocitoquímica , Humanos , Lipofuscina/metabolismo , Lisossomos/metabolismo , Lisossomos/ultraestrutura , Microscopia Eletrônica , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Ratos , Núcleo Supraóptico/enzimologia , Núcleo Supraóptico/ultraestrutura , Fatores de TempoRESUMO
The lead method was applied to determine the localization of the ATP-asic activity in the rat and human brains at different periods after death. This activity was revealed in the cytoplasm of the cells, chromatin and the nucleolus, and also in the synaptic terminals. In the vascular capillaries the product of reaction was localized in the basal layer and on the cytomembrane of the endothelial cells. The results obtained pointed to a good preservation of the ATP-asic activity in the postmortem brain.
Assuntos
Encéfalo/enzimologia , Mudanças Depois da Morte , Adenosina Trifosfatases/metabolismo , Animais , Encéfalo/metabolismo , Capilares/enzimologia , Lobo Frontal/enzimologia , Lobo Frontal/ultraestrutura , Histocitoquímica , Humanos , Hipotálamo/enzimologia , Hipotálamo/ultraestrutura , Lipofuscina/metabolismo , Microscopia Eletrônica , RatosRESUMO
By means of the electron microscopic method it became possible to study the structure of the lymph groin node (bioptic material) in 4 cases of schizophrenia and in 2 normals. All the schizophrenic patients had continuous forms (nuclear) of the disease, while their mental state during the period of examination was characterized as terminal. An analysis of the cell structure in the lymph node demonstrated that in both groups the functional activity of the lymphoid tissue was sufficiently high and in relation to some indices this activity was even higher in the group of schizophrenics (a more expressed pynocytose activity of the reticular cells and macrophages, a higher amount of blast forms of the lymphocytes, plasmatic cells and activated lymphocytes). The authors stress that these data are purely preliminary.