Fournier's gangrene in a five year old boy - beware of the child post varicella infection.

Scrotal pain and swelling are common presentations in children and are usually secondary to torsion of the testis, hydatid of Morgagni or epididymo-orchitis. Fournier's gangrene is a rare, but life-threatening disease, that can present in a similar fashion. We present a rare case of Fournier's gangrene in a 5-year-old boy associated with a preceding varicella rash.

Use of tissue ink to maintain identification of individual cores on needle biopsies of the prostate.

BACKGROUND: There is an increasing necessity to extract the maximum amount of information, beyond even a cancer diagnosis, from prostate biopsies. Thus, maintaining site-specific information regarding individual biopsy cores might be critical. AIM: To evaluate the applicability of employing tissue ink to maintain the identity of individual prostatic biopsy cores. METHOD: In this ongoing study, 12 core prostate biopsy specimens are sent to the laboratory in individual pots labelled according to anatomical site. The specimens are placed in two separate multi-compartment cassettes. They are inked with different colours to identify the site of origin from each lobe. The cassettes are then processed with a single paraffin block for each side; the six cores from each side can be mounted on a single slide. RESULTS: The different colours used adhere well to the biopsy cores, thus maintaining the identity of each core. Six cores from each side are embedded in a single paraffin block and examined on a single slide, making it cost-effective, while maintaining high quality, accurate histopathological information. CONCLUSION: Differential inking of prostate biopsy cores is an easily applicable method that is cost-effective and provides tumour location information. Prostate biopsy data archived to maintain individual core information might be used to determine applicability of such information to predict extra-capsular extension by correlating with imaging and radical prostatectomy findings, and for treatment planning.

A nidus, crystalluria and aggregation: key ingredients for stone enlargement.

The in vitro study of calcium oxalate (CaOx) stone formation is usually based on crystallisation models but it is recognised that both healthy individuals and stone formers have crystalluria. We have established a robust in vitro stone growth model based on the principle of mixed suspension, mixed product removal system (MSMPR). Utilising this technique we studied the influence of CaOx crystallisation kinetics and the variation of calcium and oxalate concentrations on CaOx stone growth in vitro. Six stones received standard concentration of Ca (6 mM) and Ox (1.2 mM) in the medium while another six received variable concentrations of both Ca and Ox at various intervals. Stone mass was plotted against the experiment duration (typically 5-7 weeks). The stone growth was dependent on sufficient input calcium and oxalate concentrations and once triggered, stone growth could not be maintained at reduced calcium and oxalate inputs. The stone growth rate was positively correlated to the number of crystals in suspension around the stone and to the crystal nucleation rate and negatively correlated to the crystal growth rates. This leads to the conclusion that aggregation of crystals from the surrounding suspension was the dominant mechanism for stone enlargement.

Effects of inositol hexaphosphate (phytate) on calcium binding, calcium oxalate crystallization and in vitro stone growth.

PURPOSE: We measured and compared 3 activities of inositol hexaphosphate, also known as phytate, to explore their importance in relation to antilithogenic potential. MATERIALS AND METHODS: Calcium binding activity by inositol hexaphosphate was measured with a calcium electrode in artificial and whole urine. Calcium oxalate crystallization inhibition was measured by a 96-well plate turbidimetric method with artificial and whole urine. Effects on stone growth were measured in an in vitro system of 12 stones grown simultaneously (a stone farm) using artificial urine alone or supplemented with urinary macromolecules. RESULTS: Phytate decreased ionized calcium, increased the metastable limit, decreased the crystallization turbidity rate index and decreased the in vitro stone growth rate. The effective concentration for calcium binding reduction was about 2 orders of magnitude higher than that required for crystallization inhibition, which in turn was about 2 orders of magnitude higher than that required for stone growth inhibition. When human urine or artificial urine supplemented with urinary macromolecules was used, the effective concentration of phytate for inhibiting crystallization and stone growth was increased by about 1 order of magnitude. CONCLUSIONS: Inhibition of crystallization by phytate does not depend on decreasing the effective ionized calcium concentration and inhibition of in vitro stone growth does not depend on inhibiting crystallization of the suspended crystals. To our knowledge this is the first demonstration of a quantitative distinction between the inhibition of crystallization and stone growth. Inhibition of in vitro stone growth in the presence of macromolecules occurred at concentrations consistent with urinary phytate excretion.

Cutaneonephrobronchial fistula: an unusual presentation of an inadequately treated perinephric abscess.

We report a case of cutaneonephrobronchial fistula secondary to an inadequately treated perinephric abscess associated with a staghorn calculus. The patient, a 72-year-old lady, was initially treated for a superficial flank abscess by incision and drainage. She returned 4 months later with a persistent discharging flank sinus, at which time the above complex pathology was diagnosed. We review similar cases in the literature and discuss pathophysiology and current management of perinephric abscesses.

Acute irritant reaction to an antiseptic bath emollient.

Antiseptic bath emollients are commonly prescribed for treatment of eczema and are generally safe for frequent application. Although acute irritant reactions are uncommon it is nevertheless recognised and could have significant morbidity. This case describes a young male patient who developed an acute irritant reaction localised to the external genitalia, mimicking Fournier's gangrene, after overnight application of Oilatum Plus antiseptic bath emollients.