Comparison of serum levels of IL-10 and IL-11 and mRNA expression of IL-10, IL-11, COX-2, BCL6, and ZEB Family in peripheral blood mononuclear cells (PBMC) of women with polycystic ovary syndrome and healthy individuals.

BACKGROUND: The roles of IL-10, IL-11, COX-2, BCL6, ZEB1, and ZEB2 genes in the potential correlation between polycystic ovary syndrome (PCOS), inflammation, and cancer remain controversial. AIMS: This study aimed to compare serum levels of IL-10 and IL-11 and gene expression of IL-10, IL-11, COX-2, BCL6, ZEB1, and ZEB2 in PBMCs of women with PCOS and healthy controls. METHODS: A case-control study included 40 women with PCOS as the case group and 40 healthy women as controls. Group matching for age and BMI was performed. Serum levels of IL-10 and IL-11 were assessed using ELISA, while gene expression was measured using real-time PCR. Parameters were compared between groups, and correlations among gene expression and serum levels were explored. RESULTS: In comparison to healthy women, women with PCOS exhibited a significant decrease in the expression of COX-2 and IL-10 genes (p<0.001), alongside a significant increase in ZEB2 gene expression (p<0.001). There were no significant differences observed in the expression of IL-11, BCL6, and ZEB1 genes. Furthermore, the serum level of IL-10 was significantly lower in women with PCOS compared to the control group (p<0.001), while no significant difference was found in IL-11 levels. Additionally, no significant correlations were identified between gene expression and serum levels. CONCLUSION: In women with PCOS, reduced IL-10 gene expression may indicate inflammation and serve as a diagnostic biomarker. However, conflicting findings on COX-2 expression complicate understanding. Elevated ZEB2 expression in PCOS women may lead to infertility, epithelial-mesenchymal transition, and aggressive phenotypes.

The effect of movement therapy with progressive muscle relaxation on the depression rate of patients admitted to the psychiatric ward of Moradi Rafsanjan Educational and Medical Center in 2021.

Depression is the most prevalent psychiatric disorder and has received more attention due to its adverse outcomes, including suicide and a severe decrease in social and individual functioning. To this end, the present study examined the effect of movement therapy and progressive muscle relaxation on the depression rate in depressed patients. In the present interventional study, 60 patients diagnosed with major depression and hospitalized at Moradi Hospital's psychiatric ward in Rafsanjan in 2020, with an age of at least 20 years, were randomly divided into two groups: the intervention group and the control group. The subjects in the intervention group attended 30 sessions of 30-45 mins, with the researcher performing a movement therapy program followed by 15 to 20 minutes of progressive muscle relaxation. The Beck Depression Inventory was used to measure the degree of depression along with clinical pre-and post-intervention interviews. The mean depression scores were 37.26±7.70 and 36.93±8.166 for the participants in the intervention group and control group before the intervention, indicating no statistically significant intergroup difference (P=0.871). The mean depression scores after the intervention for the subjects in the intervention group and control group were 8.01±5.22 and 22.96±9.43, respectively. The results showed a statistically significant difference between the groups (P=0.001), with a greater decrease in depression scores in the intervention group compared to the control group. According to the present research, movement therapy and progressive muscle relaxation interventions effectively reduced depression in patients.

Improved mine waste dump planning through integration of geochemical and mineralogical data and mixed integer programming: Reducing acid rock generation from mine waste.

Although the environmental significance of acid rock drainage (ARD) generated from mining wastes is well known, selecting the appropriate ARD management strategy can prove a complicated task. Chemical methods are favored for initial mine waste characterization but using these exclusively can overlook key factors, e.g., mineralogy, which controls the formation and elution of ARD. This paper first presents an ARD waste rock classification developed on Triple Characterization Criteria (TCC) which considers three input parameters: neutralizing potential ratio (NPR), net acid generation (NAG pH), and modal mineralogy weathering index (MMWI) values. Second, a new mixed-integer programming (MIP) model to guide waste dump construction with the dual aim of preventing ARD across the life-of-mine (LOM) and reducing waste rock re-handling, is introduced. Last, the spatial distribution of TCC in a planned waste dump is simulated via geo-statistical techniques to evaluate the MIP model. The proposed waste rock classification and dump planning model has been tested at an iron mine. The results of the MIP modeling and simulation of TCC showed the successful prevention of ARD by achieving large values of TCC (NPR ≥2, NAG pH ≥ 4.5, and MMWI ≥4.7) for dump cells, with the planned mine production maintained. The integrated TCC approach introduced in this study is intended to enable mine operators, at the start of the LOM, to effectively forecast ARD from future waste rock. Further, the MIP model will facilitate development of a mine schedule that optimizes the use of the waste materials based on TCC values. If used correctly, the TCC and MIP model have the potential to enable mine operators to reduce their environmental footprint across the entire LOM.

The effect of mindfulness-based stress reduction (MBSR) training on serum cortisol levels, depression, stress, and anxiety in type 2 diabetic older adults during the COVID-19 outbreak.

Old age is rapidly increasing and is linked to with chronic diseases, especially diabetes. Diabetes is associated with increased anxiety, stress, and depression and, in turn, can increase cortisol secretion. To this end, the present research studied the impact of mindfulness-based stress reduction (MBSR) training on serum cortisol levels, depression, anxiety, and stress in type 2 diabetic (T2DM) older adults during the COVID-19 epidemic. The participants in this interventional work were 56 older adult patients with type 2 diabetes chosen through systematic random sampling and then randomly divided into control and intervention groups containing equal members. In the intervention group, the participants attended eight mindfulness-based stress reduction (MBSR) training sessions. The subjects in the control group received no intervention. Since four patients left the study, only data for 52 patients were collected using the Depression Anxiety Stress Scale (DASS-21) and a demographic and disease information questionnaire. Data were examined with SPSS18 software using the Kolmogorov-Smirnov test, chi-square test, Fisher test, independent samples t-test, and two-way ANOVA; the significance was p<0.05. Statistically significant differences were observed between the mean scores of anxiety, stress, depression, and cortisol levels in the intervention group (p<0.00001) before, directly after, and three months after the intervention. However, no statistically significant difference was observed in the mentioned variables in the control group. The mindfulness-based stress reduction (MBSR) intervention can improve anxiety, depression, stress, and cortisol levels in older adults suffering from T2DM.

Effect of educational intervention on preventive behaviors of brucellosis among health volunteers in Rafsanjan city: Application of health belief model.

BACKGROUND: Human brucellosis can be a source of problems that affect public health, social, and economic well-being of the world's population. This study was conducted with the aim of determining the effect of Educational Intervention (EI) based on Health Belief Model (HBM) on preventive behaviors against brucellosis in Health Volunteers (HVs) in Rafsanjan. MATERIALS AND METHODS: Randomly, 104 HVs, in the intervention and control group, participated in a quasi-randomized, controlled experimental study. Variables were evaluated before and 1 month after intervention. In the intervention group, the educational program was conducted with lecture, group discussion, showing movies and related photos, booklets, and pamphlets. The program included five 45-min sessions that developed regarding the beliefs and constructs of HBM about brucellosis and its prevention methods. Data regarding HBM constructs and preventive behavior were collected using the questionnaire with 100 items by the self-report method. Finally, the data were entered into the SPSS software version 16.0, and statistical tests such as Chi-square, independent and paired t-test, Mann-Whitney, and Wilcoxon test were used for the data analysis at the significant level of 0.05. RESULTS: Prior to the intervention, the mean score of the HBM constructs and preventive behaviors between the two groups did not differ significantly, but 1 month later, in the intervention group increased significantly compared to the control group (P < 0.05). CONCLUSION: We recommend to health authorities and health-care providers to use HBM in EIs to create susceptibility, increase perceived severity and benefits, promote self-efficacy, uses cue to action, as well as reduce behavioral barriers, and ultimately adopt health-promoting behaviors.

The effect of aromatherapy with lavender essential oil on the working memory of women with multiple sclerosis.

Working memory, one of the cognitive components, may be impaired in patients with multiple sclerosis. Accordingly, this study aims to determine the effects of aromatherapy with lavender essential oil on the working memory of women with multiple sclerosis (MS). In this clinical trial, 60 women with multiple sclerosis were selected using the sampling method from patients referred to the MS Clinic of Rafsanjan. Based on the inclusion and exclusion criteria, the participants were randomly divided into intervention and placebo groups. In addition, the working memory test developed by Daneman and Carpenter was used to evaluate the participants' working memory before the intervention and the day after the last intervention. The collected data were analyzed using SPSS Statistics version 18.0. According to intragroup comparison results and based on the paired t-test, the mean score of the working memory before the intervention in the intervention group was 82.77±6.87, which increased to 87.64±5.57 after the intervention (P<0.001). The average working memory score of the placebo group was 80.30±11.09 and 82.09±11.31 before and after the intervention, respectively, which did not have a statistically significant difference (P=0.154). Based on findings from the independent t-test, the mean scores of working memory had a statistically significant difference between the intervention and placebo groups after the intervention (P=0.02). According to the results from this study, aromatherapy with lavender essential oil improved working memory in women with multiple sclerosis.

Development of a MIP model to maximize NPV and minimize adverse environmental impact-a heuristic approach.

Block sequencing is of great importance in an open-pit mining operation. Sequencing is usually performed to maximize the net present value (NPV). Also, from the environmental viewpoint, the sequence of dumping mined materials is of significant value in the sulfide mines. The potential acid-forming (PAF) waste rocks in these mines can seriously damage the environment due to the formation of acid mine drainage (AMD). To prevent the exposition of the PAF materials, it is essential to design suitable block sequencing. For this purpose, encapsulation of the PAF rocks by non-acid forming (NAF) rocks should be considered during waste dumping. However, this method can impose unnecessary re-handling costs. This issue is due to the determination of the waste-dump sequence based on improper block sequencing obtained from the previous models with the NPV maximization strategy. In the present study, a mixed-integer programming (MIP) model is proposed for generating proper block sequencing taking into account the composition of waste rocks. The main objective of the proposed MIP model is to maximize NPV and minimize the destructive environmental effects of PAF materials dumping. The CPLEX solver was applied to solve the proposed model in small datasets. Then, an artificial bee colony (ABC) is implemented to find out optimum block sequencing and waste dumping (BSWD) on a large scale. The proposed approach was examined employing several sets of data. The obtained results were compared with those of the CPLEX solver as a benchmark. An approximate gap of 2% demonstrates the efficiency of the proposed approach.

Indole itself and its novel derivative affect PML cells proliferation via controlling the expression of cell cycle genes.

Recently the role of indole and pyran rings in carcinogenesis has been well studied. Here we studied the effects and the possible mechanisms of the action of basal indole (I3A) and its novel indole derivative (C19H15F3N2O) on inhibition of proliferation cells in acute promyelocytic leukemia NB4 cell line by examining the expression of cell cycle genes. We treated NB4 cells with concentration of C19H15F3N2O for 24-72 h. The MTT and PI/Annexin V examinations were employed for assessment of the proliferation and apoptosis of NB4 cells. Both of Cyclin D and P21 were detected by the Real-time PCR. The western blotting analysis was also performed to show the protein levels for P21. A difference was regarded significant if p-value was less than 0.05. MTT assay showed that 15.12-1000 µg/mL C19H15F3N2O caused a time and concentration-dependent inhibition of NB4 cell proliferation. Exposure to higher concentrations of C19H15F3N2O resulted in significantly increased apoptosis rate in NB4 cells. RT PCR showed that C19H15F3N2O has up-regulated the expression of P21 and down-regulated the expression of Cyclin D. Western blotting experiments also demonstrated that the P21 expression in C19H15F3N2O treated cells has significantly increased, where compared with either untreated control cells or I3A treated cells. This newly (C19H15F3N2O) was able to inhibit NB4 cells proliferation and causes apoptosis of these cells more than I3A, and these effects are probably facilitated via cell cycle arrest. C19H15F3N2O might probably be introduced as a promising organic therapeutic reagent against APL.

Evaluating of OCT-4 and NANOG was differentially regulated by a new derivative indole in leukemia cell line.

BACKGROUND: The potential exists to improve treatment through characterization of tumor stem cells and identification of therapeutic targets Using OCT-4 and NANOG genes. Here we have synthesized and investigated the potential of; New Indole-3-carbaldehyde derivative (NI-3-CD) in inhibiting the expression of self-renewal regulatory factors and cancer stem cell gene in a leukemia cell line NB4. METHODOLOGY: The NB4 cells were cultured in RPMI1640 medium contained NI-3-CD and I3F (15.12-1000µg/mL) for 24, 48 and 72h. Inhibition of cell proliferation was assessed by trypan blue staining technique and MTT assay. The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V/PI apoptosis detection kit. The fold changes of NANOG/OCT4 expression against ß-actin were determined by real-time-PCR technique. Western blotting analysis was also applied for evaluating the expression of NANOG/OCT4 at protein level. Data were analyzed by student t and repeated measure tests. Differences were considered significant if (P<0.01). RESULTS: There was a significant difference in cell viability, when various concentrations of NI-3- were used for 24, 48 and 72h in comparison to I3C regarding the cellular viability. Furthermore, the NI-3-CD, had markedly elevated anticancer activity than I3C (IC50 values for novel I3C in 24, 48 and 72h were 225.77, 123.13 and 63.72M respectively while for I3C were 728.05, 407.82 and 277.92M respectively). Flow cytometry results exhibited an obviously significant augmentation in apoptotic NB4 cells. Real Time- PCR analysis indicated that the expression of NANOG/OCT4 was down regulated in compare to untreated control cells and I3C treated cells (P<0.05). In concert with RT-PCR, western blot analysis showed that the OCT4 expression in NI-3-CD treated cells was also significantly decreased in compare to both untreated control cells and I3C treated cellular populations. CONCLUSION: Our results imply that NI-3-CD treatment decreases the sphere-forming ability of NB4 cells. In summary, this study provides valuable information on the presence of stem-cell genes expression in NB4 cells.

Opium addiction as an independent risk factor for coronary microvascular dysfunction: A case-control study of 250 consecutive patients with slow-flow angina.

BACKGROUND: Approximately 20% to 30% of patients who undergo coronary angiography for assessment of typical cardiac chest pain display microvascular coronary dysfunction (MCD). This study aimed to determine potential relationships between baseline clinical characteristics and likelihood of MCD diagnosis in a large group of patients with stable angina symptoms, positive exercise test and angiographic ally normal epicardial coronary arteries. MATERIAL AND METHODS: This cross-sectional study included 250 Iranian with documented evidence of cardiac ischemia on exercise testing, class I or II indication for coronary angiography, and either: (1) angiographically normal coronary arteries and diagnosis of MCD with slow-flow phenomenon, or (2) normal angiogram and no evidence of MCD. All patients completed a questionnaire designed to capture key data including clinical demographics, past medical history, and social factors. Data was evaluated using single and multivariable logistic regression models to identify potential individual patient factors that might help to predict a diagnosis of MCD. RESULTS: 125 (11.2% of total) patients were subsequently diagnosed with MCD. 125 consecutive control subjects were selected for comparison. The mean age was similar among the two groups (52.38 vs. 53.26%, p=ns), but there was a higher proportion of men in the study group compared to control (42.4 vs. 27.2%, p=0.012). No significant relationships were observed between traditional cardiovascular risk factors (diabetes, hypertension, and dyslipidemia) or body mass index (BMI), and likelihood of MCD diagnosis. However, opium addiction was found to be an independent predictor of MCD on single and multivariable logistic regression model (OR=3.575, 95%CI: 1.418-9.016; p=0.0069). CONCLUSIONS: We observed a significant relationship between opium addiction and microvascular angina. This novel finding provides a potential mechanistic insight into the pathogenesis of MCD with slow-flow phenomenon.

Opium induces apoptosis in jurkat cells.

BACKGROUND: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. METHODS: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric detection of surface phosphatidylserine. FINDINGS: Significant differences in apoptosis of Jurkat cells were seen at 24 and 72 hours in different concentrations of opium (P < 0.05). After 72 hours, significant increase in necrosis of Jurkat cells was seen in opium concentration of 2.85 × 10-3 g/ml compared to cells without opium (control) (P < 0.05). CONCLUSION: These results showed that opium directly increases apoptosis and necrosis of T lymphocytes. This effect may play a role in immune dysfunction in opium addicts.

Comparison of serum lead level in oral opium addicts with healthy control group.

BACKGROUND: Drug abuse and its consequences are major health problems in Middle-East countries such as Iran. Salesmen and smugglers may add lead to opium during the process of opium preparation to increase the weight of opium for more profit. Several reports have found lead poisoning symptoms in opium addicted patients and there are many nonspecific symptoms mimicking lead poisoning in opium addicted patients. As far as the literature review is concerned, there is no comparative study about blood lead level (BLL) in addicted patients with healthy controls. Therefore, it seems evaluation of blood lead level in opium addicted patients to be important. METHODS: In this study, the BLL of forty-four subjects in two patient and control groups was evaluated. The patient group (22 subjects) was comprised of patients who used oral opium. Control group (22 subjects) was matched with the patient group for age and sex, considering inclusion and exclusion criteria with a mean age of 38.8+/-6.7. For blood lead assay, 3 mL of whole blood was obtained from both groups by venipuncture and BLL was assessed immediately using an atomic absorption spectrophotometer. RESULTS: The BLL in patient group had a range of 7.2 to 69.9 g/dL with a mean of 21.9+/-13.2. In the healthy control group, BLL was between 4.1 to 17.4 g/dL with a mean of 8.6+/-3.5. The mean difference of both groups (t=4.56) was statistically significant (P<0.0001). In the patient group, BLL had a significant correlation with the amount of opium ingested (r=0.65, P<0.01). However, there was no significant correlation with duration of opium ingestion in the patient group. CONCLUSION: It would be concluded that opium addicts have an elevated BLL compared to healthy controls. Therefore, screening of blood lead concentration is helpful for opium addicted people especially with non-specific symptoms. In this regard, a similar investigation with a larger sample size of opium addicted patients (including both oral and inhaled) and a control group is suggested to confirm the findings of this research.