RESUMO
Using two methods of immunization (A and B), 5/10 (A) and 27/45 (B), BALB/c by J mice immunized with human myelin proteolipid protein developed a demyelinating disease with a spectrum of chronic progressive to relapsing-remitting courses. Demyelinative lesions were seen histopathologically in all clinically affected animals that were examined. Many of the clinically unaffected animals also had histopathologic evidence of demyelination. Some of the animals had evidence of multiple ages of foci of activity. Evidence is presented that contamination with myelin basic protein could not account for the disease. This is a new model for multiple sclerosis in mice.
Assuntos
Doenças Desmielinizantes/etiologia , Modelos Animais de Doenças , Proteínas da Mielina/imunologia , Animais , Encéfalo/patologia , Doença Crônica , Doenças Desmielinizantes/metabolismo , Doenças Desmielinizantes/patologia , Feminino , Proteína Glial Fibrilar Ácida/metabolismo , Humanos , Hipersensibilidade Tardia , Imunização , Camundongos , Camundongos Endogâmicos BALB C , Proteína Básica da Mielina/metabolismo , Proteína Proteolipídica de Mielina , Neurônios/metabolismo , Neurônios/patologia , Medula Espinal/patologiaAssuntos
GMP Cíclico/metabolismo , Etanol/toxicidade , Núcleos Vestibulares/metabolismo , 3',5'-GMP Cíclico Fosfodiesterases/análise , Animais , Cálcio/fisiologia , Cerebelo/fisiologia , Guanilato Ciclase/análise , Camundongos , Neurotransmissores/farmacologia , Especificidade da Espécie , Núcleos Vestibulares/efeitos dos fármacosRESUMO
This paper reports that addition of cGMP results in an increase of the amount of(32)Pi incorporated into thiamine triphosphate in nerve roots and sympathetic trunks. The effect is present both at rest and during electrical stimulation. Other nucleotides were less effective. Theophylline increased the incorporation, possibly because of phosphodiesterase inhibition. A blocking effect of atropine was noted in sympathetic trunk preparations that contain ganglion cells.
