Service learning: a strategy for conducting a health needs assessment of the homeless.

An agency providing health care services for homeless persons and a nursing department at a liberal arts college established a service-learning partnership to complete a health needs assessment of homeless persons. Under the guidance of agency staff and a nursing faculty member, seven nursing students surveyed shelter residents (n = 101) in four urban shelters and conducted a focus group to identify residents' perceptions of health, health care needs, and health care service delivery. The service-learning partnership expanded the agency's services by providing research consultation and data collection that resulted in recommendations to improve health care services for the homeless. The agency contributed to the education of health professionals by providing students with a meaningful community service experience.

The value, be, do ethical decision-making model: balancing students' needs in school nursing.

How should school nurses balance the needs of students with complex health problems and the needs of all the other students under their care? School nurses experience this and many other stressful ethical problems. The ethical decision-making model, "Value, Be, Do: Guidelines for Resolving Ethical Conflict," provides a philosophical tool for effective resolutions and ethical nursing practice.

Effects of social support on prenatal care and health behaviors of low-income women.

OBJECTIVE: To determine the relationship of social support from partners and others to the adequacy of prenatal care and to the prenatal health behaviors of low-income women. DESIGN: Descriptive, correlational study using self-reports and medical record review. SETTING: Data were collected in five metropolitan prenatal clinics serving low-income women. PARTICIPANTS: Ethnically diverse, primarily single, low-income pregnant women (N = 101) between 28 and 40 weeks of pregnancy. MAIN OUTCOME MEASURES: Subjects completed the Norbeck Social Support Questionnaire, the Prenatal Health Questionnaire, and the Demographic/Pregnancy Questionnaire. RESULTS: Social support provided by the partner correlated positively with adequacy of prenatal care, whereas social support from others (excluding partner relationships) correlated positively with prenatal health behaviors. Professionals such as health care providers and counselors were not considered sources of social support by women. CONCLUSIONS: Nurses who work with low-income pregnant women in a variety of settings should assist partners in recognizing their potential positive contributions, teach women to communicate their expectations to their partners, acknowledge the importance of other family members as providers of social support, and when needed, refer women to programs that increase available social support.

Adjustable horizontal rectus recession surgery for disparate distance-near ocular deviations.

Strabismic deviations which display incomitance changing from distance fixation to near fixation are the result of many disturbances of both the supranuclear and infranuclear ocular motor system. This report details the surgical treatment and outcome of 6 patients with disparate distance-near deviations due to different etiologies. The surgical procedure involves recessing all four horizontal recti on both eyes using the adjustable suture technique. The approach to surgery consisted of operating on the vergence angle with the larger deviation with a bilateral lateral rectus recession (BLREC) or a bilateral medial rectus recession (BMREC) and simultaneously operating on the smaller vergence angle, treating the total induced deviation with the bilateral recession not used first (remaining BLREC or BMREC). All patients had a significant decrease in their distance-near disparity, increased horizontal comitance, normalization of their accommodative convergence to accommodation ratios, and resolution of diplopia. A four horizontal recti muscle, adjustable suture recession is another technique that can be added to the treatment regimens for distance-near disparity strabismic syndromes.

A strategy to enhance caring and community in the learning environment.

The authors describe the implementation and evaluation of faculty-facilitated small groups that encourage dialogue between nursing faculty members and students about teaching/learning concerns and events. These groups promoted student perceptions of caring behaviors from their faculty and peers and developed students' sense of cohesiveness within the learning community.

An ethical analysis of student-faculty interactions.

Senior nursing students described their interactions with faculty in a journal writing assignment. These interactions were categorized by the ethical perspectives of justice, autonomy, beneficence, and caring. An ethical analysis of faculty-student interactions is a strategy that may help nursing faculty and students understand differing perceptions about their relationships.

Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato.

The dominant gene I2 confers on tomato (Lycopersicon esculentum) resistance against the fungus Fusarium oxysporum f. sp. lycopersici race 2. A restriction fragment length polymorphism (RFLP) marker, TG105, has recently been found to be tightly linked to I2. The potential for cloning this gene by a reverse genetics approach prompted us to describe in both genetic and physical detail the region surrounding the I2 locus on chromosome 11. We have analyzed patterns of segregation of RFLP markers on chromosome 11 and Fusarium resistance in 140 F2 plants from a cross between Fusarium-resistant and susceptible parental lines. Marker TG105 mapped 0.4 centiMorgan (CM) from I2. Physical analysis of TG105 and its flanking RFLP markers, TG26 and TG36, by pulsed field gradient gel electrophoresis (PFGE) yielded a restriction map for this region encompassing at least 620 kb of the tomato genome. TG105 and TG26 hybridized to the same 175 kb MluI-NruI restriction fragment. We have therefore linked two genetically distinct RFLP markers. Based on the 4.1 cM distance between them, we have assigned a mean value of 43 kb for each cM recombination distance in the vicinity of I2. This local ratio between physical and genetic distances is more than 10-fold below the average for the tomato genome. It should therefore be possible to clone I2 by chromosome walking from TG105.

Transcriptional activation by heat and cold of a thiol protease gene in tomato.

We previously determined that low temperature induces the accumulation in tomato (Lycopersicon esculentum) fruit of a cloned mRNA, designated C14, encoding a polypeptide related to thiol proteases (MA Schaffer, RL Fischer [1988] Plant Physiol 87: 431-436). We now demonstrate that C14 mRNA accumulation is a response common to both high (40 degrees C) and low (4 degrees C) temperature stresses. Exposure of tomato fruit to 40 degrees C results in the accumulation of C14 mRNA, by 8 hours. This response is more rapid than that to 4 degrees C, but slower than the induction of many heat shock messages by 40 degrees C, and therefore unique. We have also studied the mechanism by which heat and cold exposure activate C14 gene expression. Both high and low temperature regulate protease gene expression through transcriptional induction of a single C14 gene. A hypothesis for the function of C14 thiol protease gene expression in response to heat and cold is discussed.

Analysis of mRNAs that Accumulate in Response to Low Temperature Identifies a Thiol Protease Gene in Tomato.

We have studied the induction of gene expression at low temperature by cloning mRNAs that accumulate when unripe tomato (Lycopersicon esculentum) fruit are incubated at 4 degrees C. Two cloned mRNAs, C14 and C17, accumulate relatively rapidly in response to cold treatment, while a third, C19, displays a delayed response. Significant levels of these mRNAs were not detected during fruit ripening at normal temperature. We have analyzed gene expression at different temperatures and detect half-maximal accumulation of the C14 and C17 mRNAs at 16 degrees C and 11 degrees C, respectively, and have observed that sustained gene expression requires continuous cold treatment. Furthermore, the level of C14 and C17 gene expression in cold-tolerant (hybrid L. esculentum/Lycopersicon pimpinellifolium) fruit is different from that in cold-sensitive (L. esculentum) fruit. DNA sequence analysis indicates that the C14 mRNA encodes a polypeptide with a region that is homologous to the plant thiol proteases actinidin and papain and to the animal thiol protease cathepsin H. We conclude from these experiments that low temperature selectively induces the expression of specific genes and that one such gene encodes a thiol protease.

A subpopulation of spinach chloroplast tRNA genes does not require upstream promoter elements for transcription.

We have identified a class of spinach plastid tRNA genes which do not require 5' upstream promoter elements for their expression in a chloroplast transcription system. The 5' DNA sequences flanking the trnR1 and trnS1 coding regions have little or no homology to previously characterized chloroplast promoter sequences. The deletion of the 5' DNA sequences from these genes to positions close to the start of the coding regions has little effect on their transcription in vitro. In addition, a synthetic DNA fragment homologous to the 5' region of trnS1 does not support the transcription of the promoter (-) trnM2 mutant 51 in a promoter/trnM2-51 fusion assay. In a dicistronic construct the wild type trnS1 gene does not support transcription of trnS1 transcription occurs immediately following the 3' end of the coding region. Both trnS1 and trnR1 compete with trnM2 for the same chloroplast RNA polymerase and/or common transcription factors.