Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Transplante de Células-Tronco Hematopoéticas , Seminoma/mortalidade , Seminoma/terapia , Adulto , Aloenxertos , Intervalo Livre de Doença , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Seminoma/sangue , Taxa de SobrevidaRESUMO
Reexpansion injury of the lung is rare and may occur after reexpansion of a collapsed lung such as after evacuation of a large amount of air or fluid from the pleural space. Its pathogenesis, however, is not clearly understood. Pulmonary edema should always be considered if the patient's respiratory condition worsens after lung reexpansion.
Assuntos
Tubos Torácicos , Tosse/etiologia , Dispneia/etiologia , Pneumotórax/cirurgia , Complicações Pós-Operatórias/diagnóstico por imagem , Atelectasia Pulmonar/diagnóstico por imagem , Edema Pulmonar/diagnóstico por imagem , Enfisema Pulmonar/diagnóstico por imagem , Toracostomia , Tosse/diagnóstico por imagem , Diagnóstico Diferencial , Dispneia/diagnóstico por imagem , Feminino , Humanos , Pessoa de Meia-Idade , Pneumotórax/diagnóstico por imagem , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/cirurgia , Atelectasia Pulmonar/etiologia , Atelectasia Pulmonar/cirurgia , Edema Pulmonar/etiologia , Edema Pulmonar/cirurgia , Enfisema Pulmonar/etiologia , Enfisema Pulmonar/cirurgia , Reoperação , Toracotomia , Tomografia Computadorizada por Raios XRESUMO
A PCR strategy is described for global amplification of DNA from a single eukaryotic cell that enables the comprehensive analysis of the whole genome. By comparative genomic hybridization, not only gross DNA copy number variations, such as monosomic X and trisomic 21 in single male cells and cells from Down's syndrome patients, respectively, but multiple deletions and amplifications characteristic for human tumor cells are reliably retrieved. As a model of heterogeneous cell populations exposed to selective pressure, we have studied single micrometastatic cells isolated from bone marrow of cancer patients. The observed congruent pattern of comparative genomic hybridization data, loss of heterozygosity, and mutations as detected by sequencing attests to the technique's fidelity and demonstrates its usefulness for assessing clonal evolution of genetic variants in complex populations.