Velocimetry in microchannels using photobleached molecular tracers: a tool to discriminate solvent velocity in flows of suspensions.

We report the development and analysis of a velocimetry technique based on the short time displacement of molecular tracers, tagged thanks to photobleaching. We use confocal microscopy to achieve a good resolution transverse to the observation field in the direction of the velocity gradient. The intensity profiles are fitted by an approximate analytical model which accounts for hydrodynamic dispersion, and allow access to the local velocity. The method is validated using pressure driven flow in microfluidic slits having a thickness of a few tens of micrometers. We discuss the main drawbacks of this technique which is an overestimation of the velocity close to the walls due to the combination of molecular diffusion and shear. We demonstrate that this error, limited to a near wall region of a few micrometers thick, could be controlled by limiting the diffusion of fluorophore molecules or minimizing the bleaching time. The presented technique could be combined with standard particle imaging velocimetry to access velocity differences and allow particle trajectory analysis in microflows of suspensions.

A polymeric micro-optical interface for flow monitoring in biomicrofluidics.

We describe design and miniaturization of a polymeric optical interface for flow monitoring in biomicrofluidics applications based on polydimethylsiloxane technology, providing optical transparency and compatibility with biological tissues. Design and ray tracing simulation are presented as well as device realization and optical analysis of flow dynamics in microscopic blood vessels. Optics characterization of this polymeric microinterface in dynamic experimental conditions provides a proof of concept for the application of the device to two-phase flow monitoring in both in vitro experiments and in vivo microcirculation investigations. This technology supports the study of in vitro and in vivo microfluidic systems. It yields simultaneous optical measurements, allowing for continuous monitoring of flow. This development, integrating a well-known and widely used optical flow monitoring systems, provides a disposable interface between live mammalian tissues and microfluidic devices making them accessible to detectionprocessing technology, in support or replacing standard intravital microscopy.