RESUMO
In each estrous cycle, only one follicle, the dominant follicle, reaches full maturation while the other recruited follicles become atretic in a process characteristic of programmed cell death. Moreover, the old corpus luteum formed in a previous cycle undergoes luteolysis by a mechanism also characteristic of programmed cell death. Granulosa cells comprise the largest cell population of the ovarian follicle and are the main source of estradiol and progesterone in the ovary. Their cyclic nature of differentiation and death determines the cyclic secretion of female sex hormones and therefore serve as an excellent model for steroid regulation during apoptosis. The characteristics of granulosa cell apoptosis, as in other cell types, are cell membrane blebbing, DNA degradation and protease activation. In addition, there are specific characteristics of steroidogenic granulosa cell apoptosis, as follows: 1) The trigger for apoptosis may be exerted by different effectors and signal transduction mechanisms during follicle development. For example, tumor necrosis factor (TNF) may trigger granulosa cell apoptosis at early stage of follicular development, while cAMP/p53 signals may trigger this process only in mature preovulatory granulosa cells. 2) cross-talk between paracrine and endocrine signals, and between death genes and tumor suppressor genes, may determine the fate of the granulosa cell. 3) in the mature follicle the follicular basement membrane plays an important role in transmitting survival signals and in prevention of apoptosis. 4) during the initial steps of apoptosis, steroidogenesis may be increased due to clustering of the steroidogenic organelles in the perinuclear region and their exclusion from the apoptotic blebs. 5) Actin cytoskeleton reorganization plays an important role in this compartmentalization as well as in transmitting survival signals exerted by basement membrane, laminin and growth factors which activate tyrosine kinase receptors.
