Deep eutectic solvent pretreatment of cork dust - Effects on biomass composition, phenolic extraction and anaerobic degradability.

In this study, phenolic compounds using deep eutectic solvents (DES) were extracted from cork dust, and the biogas production potential of DES-treated cork dust samples was determined. The DES treatment was carried out using choline chloride and formic acid (1:2 M ratio) at various temperatures (90, 110 and 130 °C) and treatment times (20, 40 and 60 min) at a solid-to-solvent ratio of 1:10 g mL-1. The highest total phenolic content (137 mg gallic acid equivalent (GAE) g-1 dry cork dust) was achieved at 110 °C/20 min. The extracts exhibited an antioxidant capacity of up to 56.3 ± 3.1 % 1,1-diphenyl-2-picrylhydazyl (DPPH) inhibition at a dilution rate of 100. DES treatment resulted in minimal sugar solubilization at low temperatures, while approximately 42 % of the xylan fraction in the biomass degraded under severe conditions (e.g., 130 °C/60 min). Catechin, 4-hydroxybenzoic acid and gallic acid were the major phenolics in DES extracts. The biogas yield of DES-treated cork dust increased with treatment severity. The highest biogas yield (115.1mLN gVS-1) was observed at 130 °C/60 min, representing an increase of 125 % compared to the untreated sample. SEM images revealed that the surface structure of the samples became smoother after mild pretreatment and rougher after harsh pretreatment. Compositional and FTIR analyses indicated that a higher biogas formation potential was associated with increased cellulose content in the substrate, which could be attributed to hemicellulose solubilization in the hydrolysate. Overall, DES pretreatment effectively enhanced phenol extraction and anaerobic degradability.

Process options for the recovery of a pentosan-enriched fraction from wheat-based bioethanol thin stillage.

AIM: Stillage, the main residue from cereal-based bioethanol production, offers a great potential for the recovery of pentosan-type carbohydrates. Therefore, potential process options for the recovery of pentosans from bioethanol thin stillage are investigated and their basic feasibility is demonstrated on a laboratory scale. FINDINGS: The main result of this work is the development of a three-stage process for pentosan recovery, including solid-liquid separation, pentosan solubilisation and purification. The pentosan content of the thin stillage used here was determined to be about 14% related to dry matter (DM). By means of solid-liquid separation, these pentosans accumulate in the liquid phase (up to 80%), while the remainder (about 20%) is found in the solid phase. Solubilisation of these insoluble pentosans was achieved by using either a hydrothermal, an alkaline or an enzymatic treatment. Here, the results indicate a maximum solubilisation yield of 90% with a hydrothermal treatment using liquid hot water at 180 °C. Ultrafiltration and precipitation are investigated for purification. The most promising process option in this study is solid-liquid separation followed by ultrafiltration. In this case, the total pentosan yield is assessed to be about 48% (based on thin stillage) with a final pentosan concentration of about 30%DM.

Anaerobic biogas formation from crops' agricultural residues - Modeling investigations.

The cultivation of field crops necessarily produces large quantities of organic residues, which could be used for the production of biogas. However, this is only successfully possible if the operators of such biogas plants have sufficient expertise in running these plants, e.g. to avoid an overload of the biogas system. Against this background, the anaerobic degradability of various Egyptian agricultural residues is determined by laboratory testing; this includes both a determination of degradation behavior in batch tests and a feed analysis. The residues studied produce biogas yields ranging from 303 to 496 mLN gVS-1. Co-digestion experiments demonstrate that hardly any interaction effects occur during the fermentation process for different mixtures of the investigated residues. Based on these findings, a model is developed to estimate biogas production using the investigated agricultural residues in continuous operation and to give recommendations for the optimal mode of operation.