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1.
Environ Int ; 92-93: 130-8, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27104670

RESUMO

Chile suffers significant pollution from large industrial emitters associated with the mining, metal processing, paper production, and energy industries. The aim of this research was to determine whether the presence of large industrial facilities (i.e. coal- and oil-fired power plants, pulp and paper mills, mining facilities, and smelters) affects mortality and morbidity rates in Chile. For this, we conducted an ecological study that used Chilean communes as small-area observation units to assess mortality and morbidity. Public databases provided information on large pollution sources relevant to Chile. The large sources studied were oil- and coal-fired power plants, copper smelters, pulp and paper mills, and large mining facilities. Large sources were filtered by first year of production, type of process, and size. Mortality and morbidity data were acquired from public national databases, with morbidity being estimated from hospitalization records. Cause-specific rates were calculated for the main outcomes: cardiovascular, respiratory, cancer; and other more specific health outcomes. The impact of the large pollution sources was estimated using Bayesian models that included spatial correlation, overdispersion, and other covariates. Large and significant increases in health risks (around 20%-100%) were found for communes with power plants and smelters for total, cardiovascular, respiratory, all-cancer, and lung cancer mortality. Higher hospitalization rates for cardiovascular disease, respiratory disease, cancer, and pneumonia (20-100%) were also found for communes with power plants and smelters. The impacts were larger for men than women in terms of both mortality and hospitalizations. The impacts were also larger when the sources were analyzed as continuous (production volume) rather than dichotomous (presence/absence) variables. In conclusion, significantly higher rates of total cardiovascular, respiratory, all-cancer and lung cancer mortality and cardiovascular, respiratory, cancer and pneumonia hospitalizations were observed in communes with power plants and smelters.


Assuntos
Monitoramento Ambiental , Poluentes Ambientais/toxicidade , Poluição Ambiental/efeitos adversos , Resíduos Industriais , Adulto , Teorema de Bayes , Doenças Cardiovasculares/mortalidade , Chile , Exposição Ambiental , Doença Ambiental , Poluição Ambiental/estatística & dados numéricos , Feminino , Nível de Saúde , Humanos , Masculino , Morbidade , Neoplasias/mortalidade , Doenças Respiratórias/mortalidade
2.
Rev. chil. psiquiatr. neurol. infanc. adolesc. (Impr.) ; 24(2): 118-124, ago.2013. tab, ilus
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1537912

RESUMO

Los trastornos ansiosos en la población chilena infantojuvenil son de alta prevalencia y frecuentemente subdiagnosticados. Han existido variados intentos para crear instrumentos que ayuden a su tamizaje, dentro de estos el SCARED es uno de los más utilizados. Para la población hispanoamericana se adaptó este autoreporte de niños y adolescente (AANA) con características psicométricas adecuadas. El objetivo del presente trabajo es analizar la capacidad para discriminar entre Trastorno ansioso y sus distintas dimensiones (pánico/somático, ansiedad de separación, ansiedad generalizada, fobia social y fobia escolar) del AANA en población infantojuvenil chilena no consultante. Los resultados obtenidos mostraron que el instrumento estudiado es capaz de determinar un único constructo (ansiedad) y que los distintos ítems logran determinar las 5 dimensiones encontradas en el estudio original. De acuerdo a los resultados es posible concluir que el AANA es un instrumento adecuado para pesquisar trastornos ansiosos en la población infantojuvenil chilena estudiada. Palabras Claves: Trastornos ansiosos, ansiedad, infantojuvenil, AANA, Análisis factorial exploratorio.


Anxiety disorders in chilean children and adolescents are highly prevalent and often underdiagnosed. Various attempts have been made to design tools which contribute in the screening of these conditions, among these the SCARED is one of the most popular ones. An adaptation of this instrument has been developed for hispanic population, the AANA (anxiety self-report for children and adolescents), which has proven adequate psychometric characteristics. The aim of this article is to analyzethe AANA's discriminant capacity for anxiety disorders and its various dimensions (panic / somatic, separation anxiety, generalized anxiety, social phobia and school phobia) in Chilean non consulting children and adolescents. Our results showed that this instrument was able to determine a single construct (anxiety) and its items succeeded in identifying the 5 dimensions found in the original study. According to these results, we can conclude that the AANA is a suitable instrument for anxiety disorders screening in the chilean child and adolescent population studied. Key words: Anxiety disorders, self-reporting, SCARED, exploratory factor analisys,children and adolescents.

3.
Rev Med Chil ; 141(2): 187-93, 2013 Feb.
Artigo em Espanhol | MEDLINE | ID: mdl-23732491

RESUMO

BACKGROUND: Profound changes have been observed in medical practice during the last thirty years. This may be understood as a result of the influence of economic variables in health services management, among other probable causes. At the same time, doctors' work has been diversified, and a tendency to work in several paid jobs simultaneously has been observed. AIM: To describe the characteristics of employment in a representative sample of Chilean physicians. MATERIAL AND METHODS: A probabilistic sample of 414 physicians residing in Metropolitan Santiago answered a survey about their number and type of jobs and completed the BIS UMED questionnaire that measures the subjective wellbeing of physicians. RESULTS: Forty percent of surveyed physicians had three or more jobs (36.3% of men and 47.5% of women). There was a significant inverse association between the number of jobs, general wellbeing and facing medicine from a new perspective. CONCLUSIONS: The number of jobs is inversely related to the general wellbeing of physicians.


Assuntos
Emprego/estatística & dados numéricos , Satisfação no Emprego , Corpo Clínico/estatística & dados numéricos , Carga de Trabalho/estatística & dados numéricos , Adulto , Idoso , Idoso de 80 Anos ou mais , Chile , Estudos Transversais , Emprego/psicologia , Feminino , Humanos , Masculino , Corpo Clínico/psicologia , Pessoa de Meia-Idade , Fatores Socioeconômicos , Inquéritos e Questionários , Carga de Trabalho/psicologia , Local de Trabalho
4.
Rev. méd. Chile ; 141(2): 187-193, feb. 2013. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-675060

RESUMO

Background: Profound changes have been observed in medical practice during the last thirty years. This may be understood as a result of the influence of economic variables in health services management, among other probable causes. At the same time, doctors' work has been diversified, and a tendency to work in several paid jobs simultaneously has been observed. Aim: To describe the characteristics of employment in a representative sample of Chilean physicians. Material and Methods: A probabilistic sample of 414 physicians residing in Metropolitan Santiago answered a survey about their number and type of jobs and completed the BIS UMED questionnaire that measures the subjective wellbeing of physicians. Results: Forty percent of surveyed physicians had three or more jobs (36.3% of men and 47.5% of women). There was a significant inverse association between the number of jobs, general wellbeing and facing medicine from a new perspective. Conclusions: The number of jobs is inversely related to the general wellbeing of physicians.


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Emprego/estatística & dados numéricos , Satisfação no Emprego , Corpo Clínico/estatística & dados numéricos , Carga de Trabalho/estatística & dados numéricos , Chile , Estudos Transversais , Emprego/psicologia , Corpo Clínico/psicologia , Inquéritos e Questionários , Fatores Socioeconômicos , Carga de Trabalho/psicologia , Local de Trabalho
5.
Rev. chil. salud pública ; 17(2): 119-125, 2013. graf, ilus
Artigo em Espanhol | LILACS | ID: lil-708297

RESUMO

Objetivo: Divulgar entre los investigadores en salud pública algunas propuestas de gráficos estadísticos multivariantes, tales como las caras de Chernoff, los gráficos de estrella, las curvas de Andrews y gráficos de radar. Material y método: Los gráficos estadísticos multivariantes propuestos son aplicados para describir los Sistemas de Salud Públicos de Chile, año 2010, (n=29) en base a información cuantitativa de dimensión p=7; ellos son procesados usando software estadísticos standard como R, STATA y SAS. Resultados: La descripción de los 29 Sistemas de Salud Públicos de Chile en base a información cuantitativa de dimensión siete, representados en cada uno de los gráficos multivariantes exhibidos, permite determinar conglomerados, estándares y tendencias. Conclusiones: La disponibilidad de eficientes software estadísticos sugiere complementar las indispensables estrategias de análisis exploratorio de nuestra información con representaciones gráficas adecuadas al contexto multidimensional que pueden ayudar a alcanzar una mejor comprensión del problema de interés. La síntesis gráfica generada por la aplicación de gráficos estadísticos multivariantes para describir los Sistemas de Salud Públicos de Chile complementa nuestro conocimiento de este aspecto de la realidad nacional, puede sugerir algunas hipótesis, refutar otras y ayudar en la interpretación de resultados complejos. Naturalmente esto es extensible a muchas otras situaciones de interés para el investigador salubrista.


Objective: To inform public health researches about some proposal of multivariate statistical graphics, such as Chernoff faces, star charts, Andrews curves and radar graphs. Its use is now possible thanks to recent software development. Material and method: Proposed multivariate statistical graphics are used to describe the Systems of Public Health of Chile, year 2010 (n = 29), using quantitative information with dimension p =7; these graphics are processed using standard statistical software such as R, STATA and SAS. Results: The description of the 29 systems of public health of Chile based on quantitative information from dimension seven, represented in each of multivariate graphs, allow to determine clusters, standards and trends. Conclusions: The availability of efficient statistical software indispensable suggests to complement strategies of exploratory analysis of our data with appropriate graphical representations of multidimensional context that can help us to achieve a better understanding of the problem of interest. The graphical summary generated by the application of multivariate statistical graphs to describe Public Health Systems of Chile complements our knowledge of this aspect of national life, may suggest hypotheses, refute others and assist in the interpretation of complex results. Naturally this can be extended to many other situations of interest to the health care professional.


Assuntos
Humanos , Sistemas de Saúde , Pesquisa/métodos , Modelos Estatísticos , Saúde Pública/estatística & dados numéricos , Chile , Gráficos por Computador , Expressão Facial , Análise Multivariada
6.
Rev. méd. Chile ; 139(10): 1305-1312, oct. 2011. tab
Artigo em Espanhol | LILACS | ID: lil-612198

RESUMO

Background: A change in the social structure of medicine and its impact on clients has occurred in the last decades. Aim: To perform a survey about subjective wellbeing among physicians. Material and Methods: A physician’ professional satisfaction survey consisting in 90 questions, was applied to 580 physicians (70 percent males), working in Metropolitan Santiago. Results: Physicians perceive changes in all the examined professional domains and approximately 50 percent of the changes are evaluated as negative. Change perception is a general phenomena among physicians, since there were no differences in relation to gender and only in few aspects with respect to age and medical specialty. There was a predominant positive attitude, based on the profession, to face changes. Conclusions: The positive evaluation of changes and the frequent use of managing strategies are associated with a higher satisfaction among physicians.


Assuntos
Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação no Emprego , Médicos/psicologia , Autonomia Profissional , Chile , Métodos Epidemiológicos
7.
Rev Med Chil ; 139(10): 1305-12, 2011 Oct.
Artigo em Espanhol | MEDLINE | ID: mdl-22286730

RESUMO

BACKGROUND: A change in the social structure of medicine and its impact on clients has occurred in the last decades. AIM: To perform a survey about subjective wellbeing among physicians. MATERIAL AND METHODS: A physician’ professional satisfaction survey consisting in 90 questions, was applied to 580 physicians (70% males), working in Metropolitan Santiago. RESULTS: Physicians perceive changes in all the examined professional domains and approximately 50% of the changes are evaluated as negative. Change perception is a general phenomena among physicians, since there were no differences in relation to gender and only in few aspects with respect to age and medical specialty. There was a predominant positive attitude, based on the profession, to face changes. CONCLUSIONS: The positive evaluation of changes and the frequent use of managing strategies are associated with a higher satisfaction among physicians.


Assuntos
Satisfação no Emprego , Médicos/psicologia , Autonomia Profissional , Chile , Métodos Epidemiológicos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
8.
Rev. méd. Chile ; 138(9): 1084-1090, sept. 2010. tab
Artigo em Espanhol | LILACS | ID: lil-572014

RESUMO

Background: The recent and ongoing changes in the structure and social organization of medicine have deeply transformed medical practice. Aim: To study the perception of these changes by physicians, the impact of these changes in their subjective wellbeing and their strategies of adjustment. Material and Methods: A scale, consisting of 54 items grouped in nine dimensions to measure physicians’ subjective wellbeing was devised. It was applied to a random sample of 580 physicians residing in Metropolitan Santiago and affiliated to the Colegio Médico de Chile (the Chilean Medical Association). Results: The internal consistency analysis in the instrument showed a global Cronbach´s alpha of 90 percent. Conclusions: These results support our methodological approach based on an initial qualitative identification of relevant topics in our local context, which afterwards were included as items in the scale to measure specific components of subjective wellbeing.


Assuntos
Feminino , Humanos , Masculino , Satisfação no Emprego , Padrões de Prática Médica/tendências , Médicos/psicologia , Inquéritos e Questionários/normas , Chile , Qualidade de Vida
9.
Rev Med Chil ; 138(9): 1084-90, 2010 Sep.
Artigo em Espanhol | MEDLINE | ID: mdl-21249276

RESUMO

BACKGROUND: the recent and ongoing changes in the structure and social organization of medicine have deeply transformed medical practice. AIM: to study the perception of these changes by physicians, the impact of these changes in their subjective wellbeing and their strategies of adjustment. MATERIAL AND METHODS: a scale, consisting of 54 items grouped in nine dimensions to measure physicians’ subjective wellbeing was devised. It was applied to a random sample of 580 physicians residing in Metropolitan Santiago and affiliated to the Colegio Médico de Chile (the Chilean Medical Association). RESULTS: the internal consistency analysis in the instrument showed a global Cronbach´s alpha of 90 percent. CONCLUSIONS: these results support our methodological approach based on an initial qualitative identification of relevant topics in our local context, which afterwards were included as items in the scale to measure specific components of subjective wellbeing.


Assuntos
Satisfação no Emprego , Médicos/psicologia , Padrões de Prática Médica/tendências , Inquéritos e Questionários/normas , Chile , Feminino , Humanos , Masculino , Qualidade de Vida
10.
Rev. psiquiatr. clín. (Santiago de Chile) ; 45(1/2): 48-58, dic. 2008. tab
Artigo em Espanhol | LILACS | ID: lil-532988

RESUMO

Se realizó un estudio transversal- explicativo de las propiedades psicométricas y la validación estructural de estandarizar la Escala de Ansiedad en niños y adolescentes (SCARED) traducida y posteriormente validada en México, en población infanto-juvenil entre 8 y 15 años de edad (AAA). Se aplicó la escala a 128 niños escolarizados de ambos sexos entre los 7 y 17 años, consultantes a un centro privado de salud mental en Santiago, Chile. El plan de análisis consideró la evaluación de la consistencia interna (alfa de Cronbach), la capacidad discriminativa de cada ítem y la validez de constructo, usando el análisis factorial a partir de los componentes principales con rotación Varimax. Se determinó la adecuación del modelo factorial mediante el índice de Kaiser-Meyer-Olkin (KMO). Se consideró, además, que la distribución de frecuencia para las opciones de respuesta de cada ítem de la escala fuera homogénea.La escala queda finalmente conformada por 38 ítems, los cuales cumplen todos con el criterio de discriminación y homogeneidad. La consistencia interna de la escala obtenida fue 0.88. Con el análisis se formaron 6 factores que agrupan características clínicas. Conclusión: la escala de ansiedad AAA es un instrumento útil por ser breve y de fácil aplicación, permitiendo identificar síntomas de ansiedad en la muestra infanto-juvenil estudiada.


We perfomed a cross-sectional analytical study of psychometric characteristics and structural validation of the anxiety scale for children and adolescents (SCARED) translated and validated in Mexico, on a population of children and adolescents aged between eight to fifteen years old (AAA) .The scale was applied on 128 students, males and females, aged seven to seventeen years old, attending to a private center of mental care in Santiago, Chile. The plan of analysis considered to assess the internal consistency (Cronbach alpha), discriminative capacity of each item and the validation of construct using factorial analysis based on principal components with varimax rotation We evaluated the factorial model by means of Kaiser-Meyer-Olkin index (KMO). We also considered the homogeneity, frequency distribution of the answers of each item. The final scale was made up of 38 items that satisfied the discriminative and homogeneity criteria. Internal consistency for the total scale was high (Cronbach’s á = 0.88).The factorial analysis found 6 factors that explain 79.9 per cent of the total variability. Each factor is related to one specific clinical characteristic Coclusions: The anxiety scale for children and adolescents (AAA) would be a good instrument for measuring and identifying clinical characteristics of anxiety in children and adolescents.


Assuntos
Humanos , Masculino , Adolescente , Feminino , Criança , Ansiedade/diagnóstico , Ansiedade/psicologia , Escalas de Graduação Psiquiátrica , Chile , Distribuição por Sexo , Estudos Transversais , Psicometria , Reprodutibilidade dos Testes
11.
Maturitas ; 61(3): 248-51, 2008 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-18818032

RESUMO

OBJECTIVES: To evaluate the impact of low-dose oral estrogen therapy on the health-related quality of life (HRQoL) in 45-64-year-old women from the East Metropolitan Health Service (SSMO) in Santiago, Chile. MATERIAL AND METHODS: We conducted an observational cross-sectional study. A random population sample of women between 45 and 64 years of age was obtained through an invitation to contact one of 15 primary health care centers of the SSMO of Santiago, Chile. Out of the 927 women who were originally contacted, 844 women were able to complete the Menopausal Rating Scale (MRS) questionnaire. Information about demographic parameters, health issues, and modality of hormonal therapy (HT) were registered. Three groups were compared: group 1 (n=647; non-users of HT), group 2 (n=82; users of low-dose oral estrogen HT), and group 3 (n=115; users of non low-dose estrogens HT). RESULTS: There were no differences among groups in terms of demographic and health issue parameters. The results of the MRS scores (total score and somatic, psychological and urogenital domain scores) showed significant differences across the 3 study groups, with more favorable results for HRQoL in groups 2 and 3 (p<0.01 for total, somatic, and psychological scores; p=0.05 for urogenital score). CONCLUSION: Climacteric women in the 45-64 age range using HT were shown to have a more favorable impact on HRQoL than non-HT users. Women using low-dose oral estrogen HT had a positive effect on HRQoL, similar to that obtained using non low-dose estrogen regimens.


Assuntos
Estradiol/análogos & derivados , Estradiol/administração & dosagem , Terapia de Reposição de Estrogênios/métodos , Estrogênios Conjugados (USP)/administração & dosagem , Menopausa/efeitos dos fármacos , Chile , Estudos Transversais , Terapia de Reposição de Estrogênios/psicologia , Feminino , Humanos , Menopausa/psicologia , Pessoa de Meia-Idade , Qualidade de Vida , Estatísticas não Paramétricas
12.
Biol Res ; 38(1): 7-12, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15977405

RESUMO

Longitudinal studies aimed at evaluating patients clinical response to specific therapeutic treatments are frequently summarized in incomplete datasets due to missing data. Multivariate statistical procedures use only complete cases, deleting any case with missing data. MI and MIANALYZE procedures of the SAS software perform multiple imputations based on the Markov Chain Monte Carlo method to replace each missing value with a plausible value and to evaluate the efficiency of such missing data treatment. The objective of this work was to compare the evaluation of differences in the increase of serum TNF concentrations depending on the -308 TNF promoter genotype of rheumatoid arthritis (RA) patients receiving anti-TNF therapy with and without multiple imputations of missing data based on mixed models for repeated measures. Our results indicate that the relative efficiency of our multiple imputation model is greater than 98% and that the related inference was significant (p-value < 0.001). We established that under both approaches serum TNF levels in RA patients bearing the G/A -308 TNF promoter genotype displayed a significantly (p-value < 0.0001) increased ability to produce TNF over time than the G/G patient group, as they received successively doses of anti-TNF therapy.


Assuntos
Anticorpos Monoclonais/uso terapêutico , Antirreumáticos/uso terapêutico , Artrite Reumatoide/sangue , Artrite Reumatoide/tratamento farmacológico , Modelos Estatísticos , Fator de Necrose Tumoral alfa/análise , Artrite Reumatoide/genética , Genótipo , Humanos , Infliximab , Método de Monte Carlo , Análise Multivariada , Reação em Cadeia da Polimerase , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Regiões Promotoras Genéticas/efeitos dos fármacos , Regiões Promotoras Genéticas/genética , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/genética
13.
Biol. Res ; 38(1): 7-12, 2005. ilus, tab
Artigo em Inglês | LILACS | ID: lil-404822

RESUMO

Longitudinal studies aimed at evaluating patients clinical response to specific therapeutic treatments are frequently summarized in incomplete datasets due to missing data. Multivariate statistical procedures use only complete cases, deleting any case with missing data. MI and MIANALYZE procedures of the SAS software perform multiple imputations based on the Markov Chain Monte Carlo method to replace each missing value with a plausible value and to evaluate the efficiency of such missing data treatment. The objective of this work was to compare the evaluation of differences in the increase of serum TNF concentrations depending on the ¡308 TNF promoter genotype of rheumatoid arthritis (RA) patients receiving anti-TNF therapy with and without multiple imputations of missing data based on mixed models for repeated measures. Our results indicate that the relative efficiency of our multiple imputation model is greater than 98 percent and that the related inference was significant (p-value < 0.001). We established that under both approaches serum TNF levels in RA patients bearing the G/A ¡308 TNF promoter genotype displayed a significantly (p-value < 0.0001) increased ability to produce TNF over time than the G/G patient group, as they received successively doses of anti-TNF therapy.


Assuntos
Humanos , Anticorpos Monoclonais/uso terapêutico , Antirreumáticos/uso terapêutico , Artrite Reumatoide/tratamento farmacológico , Modelos Estatísticos , Regiões Promotoras Genéticas , Fator de Necrose Tumoral alfa , Artrite Reumatoide/sangue , Genótipo , Método de Monte Carlo , Análise Multivariada , Reação em Cadeia da Polimerase , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Regiões Promotoras Genéticas , Fator de Necrose Tumoral alfa
14.
Eur Cytokine Netw ; 15(4): 364-70, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15627647

RESUMO

Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-alpha gene promoter. The transition G-->A at position -308 generates the TNF-alpha1 (G/G) and TNF-alpha2 (G/A or A/A) alleles, where the polymorphic TNF-alpha2 allele is associated with a high, in vitro TNF-alpha expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-alpha SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-alpha capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-alpha promoter was performed by PCR-RFLP analysis. For TNF-alpha expression we used a blood culture system.


Assuntos
Diabetes Mellitus Tipo 1/genética , Leucócitos/metabolismo , Periodontite/genética , Polimorfismo de Nucleotídeo Único/genética , Regiões Promotoras Genéticas/genética , Fator de Necrose Tumoral alfa/genética , Adulto , Diabetes Mellitus Tipo 1/complicações , Diabetes Mellitus Tipo 1/metabolismo , Feminino , Predisposição Genética para Doença/genética , Humanos , Lipopolissacarídeos/farmacologia , Masculino , Periodontite/complicações , Periodontite/metabolismo , Polimorfismo de Nucleotídeo Único/fisiologia , Regiões Promotoras Genéticas/fisiologia , Fator de Necrose Tumoral alfa/biossíntese
15.
Eur Cytokine Netw ; 14(3): 128-33, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14656685

RESUMO

Several studies have demonstrated that diabetes is a risk factor for developing periodontal disease, increasing its prevalence and severity. Furthermore, periodontitis may impair the metabolic control and adequate treatment of diabetic patients. LPS from Gram-negative bacteria penetrates the periodontal tissues and subsequently recruits and activates immune cells. Progression to severe periodontitis with loss of supporting structures is mediated by several factors, including secretion of a broad spectrum of inflammatory and destructive (PGE2). mediators such as cytokines (TNF-alpha, IL-1b and IL-6), chemokines (IL-8) and prostaglandin E2. The aim of this work is to investigate differences in the TNF-a, IL-1b and IL-6 expression and prostaglandin E2 (PGE2) release in blood from diabetic patients with and without aggressive periodontitis (AP) stimulated with lipopolysaccharide (LPS). For this purpose we recruited 29 Type 1 diabetes mellitus (DM) patients, 14 with AP and 15 without AP. Fourteen healthy individuals formed the control group. For cytokine expression and PGE2 secretion, an ex vivo whole blood culture system was used. Cytokines and PGE2 were detected by commercial immunometric assays. A wide range of inter-individual variability in spontaneous and LPS-induced TNF-alpha, IL-1b and IL-6 levels in patient groups and controls was found. The mean of spontaneous and LPS-induced TNF-alpha and IL-1b levels did not differ significantly (p > 0.5) when patients were compared to control individuals. Although not significant, the spontaneous TNF-alpha, IL-1b and IL-6 levels in the group of Type 1 DM with AP were higher than in controls, while in diabetic patients without AP, these values were depressed in comparison with controls. In both groups of patients, the means of LPS-induced IL-6 levels were higher than the controls but the differences observed were not significant (p = 0.07). However, the LPS-induced PGE2 levels varied significantly when all groups were compared (p = 0.007). The means of LPS-induced PGE2 levels for Type 1 diabetic patients with AP (p = 0.0009) and without AP (p = 0.024) were significantly higher than the levels observed for healthy controls. Finally, we conclude that Type 1 diabetic patients with or without AP did not express higher LPS-induced TNF-a, IL-1b and IL-6 levels than controls. However, the PGE2 levels released were significantly higher than those detected in controls.


Assuntos
Diabetes Mellitus Tipo 1/sangue , Dinoprostona/metabolismo , Interleucina-1/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Periodontite/complicações , Adulto , Diabetes Mellitus Tipo 1/complicações , Feminino , Humanos , Técnicas In Vitro , Masculino
16.
Biol. Res ; 34(3/4): 237-241, 2001. tab
Artigo em Inglês | LILACS | ID: lil-303887

RESUMO

Several single-nucleotide polymorphisms have been identified in the human TNF gene promoter. The polymorphism at position-308 (TNF-308), which involves substituting G for A and designing the TNF2 allele, leads to a higher rate of TNF gene transcription than the wild-type TNF1 allele in in vitro expression studies. It has also been linked to increased susceptibility to a variety of illnesses. Using PCR-RFLP analysis we detected significant differences in the TNF-308 genotypes of Chilean and other populations. We conclude that there is a gradient in the distribution of the TNF2 allele according to ethnicity; we have also hypothesized that populations bearing a higher proportion of the TNF2 allele may have an increased predisposition toward or incidence of several chronic metabolic, degenerative, inflammatory and autoimmune diseases.


Assuntos
Humanos , Alelos , Frequência do Gene , Predisposição Genética para Doença , Polimorfismo Genético , Regiões Promotoras Genéticas , Fator de Necrose Tumoral alfa , Chile , Predisposição Genética para Doença , Genótipo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
17.
Rev. neuro-psiquiatr. (Impr.) ; 60(2): 106-115, jun. 1997. tab
Artigo em Espanhol | LILACS | ID: lil-343471

RESUMO

Se estudia la relación entre elección temática espontánea de un individuo, su género y su nivel socioeconómico, aplicando el método de análisis de datos discretos conocido como "Análisis de Correspondencias". 377 individuos, 115 mujeres y 262 hombres, clasificados por la Escala Social de Graffar según nivel socioeconómico (medio alto, medio y medio bajo) fueron invitados a narrar espontáneamente alguna circunstancia o suceso importantes de sus vidas. Los temas aludidos fueron reducidos a una lista de doce. Los primeros "valores propios" 0.11 y 0.05 de este análisis, explican más del 70 por ciento de la asociación total de las variables. La presentación de la informaciónen el plano vincula la elección de temas con el nivel socioeconómico de género de los individuos. los del nivel socioeconómico medio alto con los temas recreación, Trabajo y enfermedad en posición a los del nivel socioeconómico bajo que se relacionarian con los temas accidentes y servicio militar. Las mujeres del nivel socioeconómico medio se identificarian con los temas : Religión, Familia de Origen y otros, en contraste con las mujeres pertenecientes al nivel socioeconómico medio alto, que se vincularian con temas relacionados con la familia: Muerte natural y accidental, educación, Paternidad-Maternidad y Relación de pareja


Assuntos
Classe Social , Sexo , Enquete Socioeconômica , Correspondência como Assunto
18.
Rev. chil. salud pública ; 1(1): 17-21, 1997. tab, graf
Artigo em Espanhol | LILACS | ID: lil-277940

RESUMO

Este trabajo describe similitudes en las variables del Cuestionario de Conducta Alimentaria (Restricción, Desinhibición, Hambre), el Cuestionario de Personalidad de Eysenck (EPQ_R) (Neuroticismo, Extraversión, Psicoticismo y Escala de Mentira) y la Escala de Alexitimia de Toronto en 11 díadas madre-hija primogénitas, una de cuyos integrantes consultó por sobrepeso. Se usó la prueba de Wilcoxon para muestras pareadas y los coeficientes de correlación de rangos de Spearman. No hay diferencias estadísticamente significativas entre las medias de estas evaluaciones. Las correlaciones significativas entre las variables coinciden en ambos grupos. El estudio multidimencional se basó en un análisis de componentes principales no paramétrico con individuos suplementarios. Las tres primeras componentes principales resumen el 80,4 por ciento de la variabilidad de los datos ordenados según rangos en el grupo de las madres, que se correlacionan con las respectivas evaluaciones en las hijas


Assuntos
Humanos , Feminino , Comportamento Alimentar/psicologia , Obesidade/genética , Transtornos da Personalidade/psicologia , Transtornos de Ansiedade/psicologia , Índice de Massa Corporal , Fome/fisiologia , Obesidade/psicologia , Sintomas Afetivos/psicologia , Fatores Socioeconômicos , Inquéritos e Questionários/estatística & dados numéricos
19.
Rev. argent. clín. psicol ; 5(2): 177-185, ago. 1996. tab
Artigo em Espanhol | BINACIS | ID: bin-1705

RESUMO

Este trabajo describe la expresión verbal de hostilidad mediante el método de análisis de contenido de Gottschalk y cols. en 96 mujeres que presentaban el antecedente de intento de suicidio, dividiendo este grupo en mujeres que reiteran una tentativa autolítica (n=56) y las que efectúan el intento por primera vez (n=40). Los resultados muestran que el mayor promedio corresponde a Hostilidad dirigida hacia adentro y el menor a Hostilidad dirigida haca afuera encubierta. Las medias de expresión de hostilidad en los grupos de repetidoras versus no repetidoras no mostraron diferencias significativas. En los análisis ed correlaciones aparece como relevante que en el grupo de las no repetidoras, la Hostilidad hacia afuera manifiesta se correlaciona en forma directa con: no arrepentimiento frente al intento y la Escala de Intención Suicida;...(AU)


Assuntos
Humanos , Feminino , Hostilidade , Tentativa de Suicídio/psicologia , Mulheres/psicologia , Idioma
20.
Rev. argent. clín. psicol ; 5(2): 177-185, ago. 1996. tab
Artigo em Espanhol | LILACS | ID: lil-406062

RESUMO

Este trabajo describe la expresión verbal de hostilidad mediante el método de análisis de contenido de Gottschalk y cols. en 96 mujeres que presentaban el antecedente de intento de suicidio, dividiendo este grupo en mujeres que reiteran una tentativa autolítica (n=56) y las que efectúan el intento por primera vez (n=40). Los resultados muestran que el mayor promedio corresponde a Hostilidad dirigida hacia adentro y el menor a Hostilidad dirigida haca afuera encubierta. Las medias de expresión de hostilidad en los grupos de repetidoras versus no repetidoras no mostraron diferencias significativas. En los análisis ed correlaciones aparece como relevante que en el grupo de las no repetidoras, la Hostilidad hacia afuera manifiesta se correlaciona en forma directa con: no arrepentimiento frente al intento y la Escala de Intención Suicida;...


Assuntos
Humanos , Feminino , Hostilidade , Tentativa de Suicídio , Mulheres , Idioma
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