RESUMO
INTRODUCTION: The extracellular matrix (ECM) is a complex, tissue-specific 3-dimensional network that controls cell processes. ECMs derived from various organs are used to produce biological scaffolds comparable to the native microenvironment. Although placentas are often overlooked, they offer a rich ECM for tissue engineering, especially the hemochorial placentas from rodents and lagomorphs that resemble the ones from humans. METHODS: Here we established a protocol for decellularization and investigated the ECM in native and decellularized placentas of guinea pigs, rats and rabbits by means of histology, immunohistochemistry, immunofluorescence and scanning electron microscopy. RESULTS: Effective decellularization were achieved by immersion in 0.25% Sodium Dodecyl Sulfate for 3 days, resulting in an intact ECM, while cells or nuclei were absent. All species had a high diversity of ECM components that varied between areas. DISCUSSION: Dense fibrous networks in the junctional zone were strongly positive to collagen I, III and IV, fibronectin, and laminin ECM markers. Noticeable response were also found for the decidua, especially along the maternal vessels. The labyrinth had thin fibers strongly positive for fibronectin and laminin, but not much for collagens. In conclusion, we established an effective protocol to obtain biological scaffolds from animal models with hemochorial placentas that possessed promising values for future purposes in Regenerative Medicine.
