RESUMO
An aerobic, Gram-negative bacterial isolate, strain DSM 19503(T), was isolated from haemolymph serum of the blacklip abalone Haliotis rubra. Cells of strain DSM 19503(T) were vibrioid to spiral, motile and were able to pass through sterile filters with a pore size of 0.2 microm, indicating the small width of the bacterium. The isolate was psychrophilic, with the ability to grow at 2-8 degrees C. Oxidase activity was present, whereas catalase activity was absent. The nearly complete 16S rRNA gene sequence of strain DSM 19503(T) was obtained and phylogenetic sequence analysis showed that it formed a distinct genus in the family Oceanospirillaceae with highest sequence similarity of 92.9% to Oleispira antarctica RB-8(T). The cellular fatty acid composition was dependent on the growth medium used for cultivation. During growth on seawater agar, the fatty acid composition was most similar to that of Oleispira antarctica DSM 14852(T), with mainly C(16:0) (90.3%). In contrast, Columbia blood agar/NaCl-grown cells exhibited mainly C(10:0) 3-OH (11.8%), C(12:1)cis5 (8.2%), C(16:1)cis9 (29.6%), C(16:0) (19.3%) and C(18:1)cis9 (13.1%) fatty acids. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DSM 19503(T) is considered to represent a novel species in a new genus for which the name Oceaniserpentilla haliotis gen. nov., sp. nov. is proposed. The type strain of Oceaniserpentilla haliotis is DSM 19503(T) (=LMG 24225(T)).
Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Gastrópodes/microbiologia , Soro/microbiologia , Aerobiose , Animais , Bactérias/genética , Fenômenos Fisiológicos Bacterianos , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Genes de RNAr , Locomoção , Dados de Sequência Molecular , Oxirredutases/metabolismo , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , TemperaturaRESUMO
Indanofan and its analogs inhibited the elongation of stearoyl- or arachidoyl-CoA by [2-14C]-malonyl-CoA in leek microsomes from Allium porrum. Although the precise mode of interaction of indanofan at the molecular level is not completely clarified by the present study, it is concluded that indanofan and analogs act as inhibitor of the elongase enzyme involved in de novo biosynthesis of fatty acids with an alkyl chain longer than C18, called very-long-chain fatty acids (VLCFAs). For a strong inhibition of VLCFA formation chloro substituents at the benzene ring and the oxirane group were necessary. Furthermore, the greenhouse test showed strong activity for indanofan and its analogs, and the scores coincided with cell-free elongation inhibition. The cell-free assay, however, failed to indicate any activity for an analog having a methylene instead of the oxirane group, while both Digitaria ciliaris and Echinochloa oryzicola were killed with 1 kg a.i./ha. This finding cannot be discussed because the applied use rate of 1 kg a.i./ha is too high to allow for a score differentiation. For high concentrations of this compound additional unknown inhibitory effects may be involved besides fatty acid elongation.
