RESUMO
The analytical and descriptive color graphics capabilities (the GEM environment) of the CAGE/GEM(a) software system are described using bacteriophage lambda (48,502 base pairs) and Epstein Barr virus (172,282 base pairs) as examples. Genetics and features, as graphic drawings, and the results of sequence analysis as pseudo-colored representations, are simultaneously displayed at all zooming levels. The present upper limit on sequence size is 5 x 10(5) bases. The overlay editor utility which provides the capability to structure complex sequence associated data and knowledge bases into a manageable color graphics format is also described. Holistic correlations that can be made by the display of bacteriophage lambda and Epstein Barr virus in the GEM environment are also discussed.
Assuntos
Sequência de Bases , Gráficos por Computador , Computadores , Microcomputadores , Ácidos Nucleicos , Bacteriófago lambda/genética , DNA Viral/genética , Genes Virais , Herpesvirus Humano 4/genética , Sistemas de Informação , Software/métodosRESUMO
The CAGE/GEM(TM) software toolkit for genetic engineering is briefly described. The system functionally uses color graphics and is menu driven. It integrates genetics and features information ("Overlays") with information based on sequence analysis ("Representations"). The system is structured around CAD (Computer Aided Design) principles. The CAGE (Computer Aided Genetic Engineering) aspects of the software are emphasized and illustrated by a simulated cloning of the hepatitis B core antigen gene into the BAMHI site of plasmid pBR322.
