RESUMO
A simple automatic counting device for cells on Petri dishes is described. The device is tested up to 1 x 10(4) cells (0.01 m)-2 and counts linearly without coincidence error. The counting time for each dish is c. 3-4 min including the time required to place the dish in the apparatus.
Assuntos
Autorradiografia , Contagem de Células/instrumentação , Células CultivadasRESUMO
To elucidate the process of contact inhibition in mammalian cells, we investigated the kinetics of growth arrest in [3H]thymidine labelled embryonic chinese hamster (Cricetulus griseus L.) cells after the addition of various concentrations of unlabelled cells. It was observed that after the contact inhibition concentration had been reached, the cells grew undisturbed for one more generation. In the following 24 hr the concentration fell back to the level at the beginning of the experiment and stayed there.
Assuntos
Inibição de Contato , Animais , Divisão Celular , Linhagem Celular , Transformação Celular Neoplásica , Células Cultivadas , Cricetinae , Cinética , Timidina , TrítioRESUMO
A chemostat in which mammalian cells can be raised in continuous suspension culture is described. It is constructed from commercially available parts. This apparatus has the advantage over earlier models in that the medium can be pumped off free of cells, thus suddenly increasing the cell concentration in the culture. The apparatus has been successfully used in studies on contact inhibition.
