The Influence of Barley Proteome on Hop Bitter Acid Yield during Brewing.

A persistent challenge in brewing is the efficient utilization of hop bitter acids, with about 50% of these compounds precipitating with trub during wort boiling. This study aims to uncover the correlation between the barley cultivar proteome and hop bitter acid utilization during wort boiling. Therefore, comparative experiments were conducted using two cultivars, Liga and Solist, with varying proteomes to identify specific proteins' role in hop bitter acids precipitation. High-performance liquid chromatography (HPLC) was used to measure hop bitter acid content, while liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify and identify proteins. The 107 protein groups, particularly enzymes linked to barley metabolic defense mechanisms, exhibited significant differences between the two cultivars. Results revealed significantly lower α- and iso-α-acid content in wort produced from the barley cultivar Liga. This study highlights the critical role of the barley proteome in optimizing process efficiency by enhancing hop utilization through barley cultivar selection.

Addition of Hop (Humulus Lupulus L.) Bitter Acids Yields Modification of Malt Protein Aggregate Profiles during Wort Boiling.

Hop bitter acids are used in the brewing industry to give beer bitterness. However, much of this bitterness is lost during processing, specifically during the wort boiling step. One of the major causes might be the interaction with protein-protein complexes. Therefore, the aim of this study was to clarify the role of hop bitter acids in protein aggregate formation using a proteomic approach. The effect of hop addition on protein composition was analyzed by liquid chromatography-mass spectrometry/MS (LC-MS/MS), and further analyses were performed to characterize the wort before and after boiling. Addition of hop bitter acids yielded a change in wort protein profiles, and hop bitter acids were found to bind primarily to less abundant proteins which are not related to beer quality traits, such as foam or haze. Wort protein aggregate profiles were revealed, and findings from this study suggested the precipitation of particular proteins in the aggregates during boiling when hops were added.

Effect of the homogenization process on the sensory and rheological properties in model system.

High pressure homogenization has been used for preparation and stabilization of emulsions and suspensions, promoting physical changes in products, such as viscosity change. However, its use in model systems for fruit nectar is innovative. This study aimed to evaluate the effect of the processing in high pressure homogenizer on the rheological behavior and the sensory attributes in model system of gellan gum. Gellan gum (0.05%), organic acids (0.3%), and sucrose (10%) were used to prepare the solutions, which were subsequently homogenized (0-control, 25 and 50 MPa) at 25°C. Rheological and sensory analyses were performed. The samples presented pseudoplastic behavior without residual stress and were characterized by the Ostwald-de-Waele model. The homogenization pressure (PH ) altered the viscosity of the model systems, reduced the consistency index and apparent viscosity, and increased the flow behavior index. The stimuli sour taste and viscosity differed among the parameters evaluated in the time-intensity analysis. No differences were observed for the maximum intensity of viscosity between the treated samples (25 and 50 MPa), which exhibited a similar temporal profile. Therefore, studies on the rheological and sensory behavior are fundamental to product development and process optimization. The present study shows new trends on the use of the high pressure homogenizer and the sensory profile in model systems that can be used for fruit nectar. This study evaluated the sensory attributes through the time-intensity analysis. The sensory data indicate that the homogenization pressure altered the viscosity perception, but did not alter the perception of the acid taste for the same organic acid used. Sensory as well as rheological data present new alternatives for product development. These findings are interesting for future research, equipment design, and process optimization.

Application of time-intensity analysis in model system submitted to homogenization.

The use of the high pressure homogenizer has been studied in fruit juices, but researches in model system for application in fruit nectar are scarce. Therefore, it is necessary to evaluate the application of these technologies and how the homogenization pressure (PH) can interfere in the sensorial profile of the samples. To prepare the solutions we used guar gum (0.1%), organic acids (0.3%), and sucrose (10%), which were later homogenized (0-control, 25 and 50 MPa) at 25 ℃. The rheological behavior and the temporal profile of the samples were evaluated. The model systems presented pseudoplastic behavior without residual tension and were fitted to the Ostwald-de Waele model. The consistency index reduced and the flow behavior index increased with processing. Apparent viscosity also decreased due to homogenization. In the time-intensity sensorial analysis, it was observed that the samples differed among the evaluated parameters, demonstrating that the samples with tartaric acid presented higher intensity for the sour taste. However, for sweetness, no change was observed. In the viscosity attribute, the model systems presented similar temporal profiles. Therefore, it was noted that the homogenization process favored a greater temporal profile of sour taste, making sensory perception more lasting in a model system for fruit nectar.

Rheological analysis of sucrose solution at high temperatures using a microwave-heated pressurized capillary rheometer.

Thermal process design, optimization, and deviation require the knowledge of engineering properties of food, such as rheological behavior, which is highly dependent on temperature. If a process is calculated based on the parameters obtained by extrapolation, it might lead to over- or underprocessing. The long time required for the product to reach the desired temperature, which is usual in many rheometers, may induce unexpected changes on the product. Model solutions are often used in engineering projects in order to simulate characteristics of real products. Thus, the objective of this study was to obtain rheological data of sucrose model solution at the actual high UHT (ultra-high temperature) process temperatures using a pressurized capillary rheometer, heated by a microwave system. The results provide the rheological behavior of the sucrose solutions and show that there is no deviation of the linearity of the Arrhenius plot above 100 °C for this product. This work was useful to illustrate the use of the applied methodology and to validate the newly built microwave-heated capillary rheometer. The data obtained confirm the stability of nonsaturated sucrose solution when treated above 100 °C and presents an important contribution to the literature database, since sucrose solutions are used as models for many table syrups, such as maple and sorghum syrups.

Boas práticas agrícolas e aplicação do sistema APPCC no cultivo e processamento industrial do café obtido pela via seca / Good agricultural practices and application the HACCP in the processing of roasted and milled coffee

O Brasil é o maior produtor e exportador mundial de café. A maior parte do café brasileiro é exportado como café verde (ou crú) e é adquirido e apreciado no país principalmente como café torrado e moído. Há uma preocupacção mundial crescente com relação a segurança e qualidade dos alimentos. O sistema de análises de Perigos e Pontos Críticos de Controle (APPCC) tem sido indicado como o método mais eficiente para garantir a qualidade e segurança alimentar. Assim sendo, o objetivo foi aplicar o referido sistema no processo de obtenção do café torrado e moído, produzido por via seca, no Brasil. As etapas de secagem, armazenamento e transporte foram consideradas os pontos críticos de controle devido à possibilidade de contaminação com ocratoxina. Além disso, a etapa de torração foi relacionada com a perda de qualidade do produto final. Medidas de controle e limites críticos foram indicados e dependem principalmente de conhecimentos de pós-colheita do produto.