RESUMO
Blood cultures from a heroin user who died in June 2012, a few hours after hospital admission, due to acute septic disease, revealed the presence of Bacillus anthracis. This report describes the extended diagnosis by MALDI-TOF and real-time PCR and rapid confirmation of the anthrax infection through reference laboratories. Physicians and diagnostic laboratories were informed and alerted efficiently through the reporting channels of German public health institutions, which is essential for the prevention of further cases.
Assuntos
Antraz/diagnóstico , Antraz/etiologia , Bacillus anthracis/isolamento & purificação , Bacteriemia/etiologia , Contaminação de Medicamentos , Heroína , Abuso de Substâncias por Via Intravenosa/complicações , Bacillus anthracis/genética , Usuários de Drogas , Evolução Fatal , Genoma Bacteriano , Alemanha , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real , Sepse/etiologiaAssuntos
Fístula Cutânea/etiologia , Equinococose Hepática/complicações , Idoso , Animais , Antígenos de Helmintos/sangue , Fístula Cutânea/cirurgia , Equinococose Hepática/diagnóstico , Equinococose Hepática/patologia , Echinococcus/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Resultado do TratamentoRESUMO
Pneumocystis jirovecii causes severe pneumonia (PCP) in immunocompromised patients. Seasonal changes of PCP incidence may be associated with climate changes. In this first study using multiple linear regression statistics to assess monthly climatic data and Pneumocystis, PCP incidence was positively correlated with mean temperature, but not with rainfall or wind strength.
Assuntos
Clima , Infecções por Pneumocystis/epidemiologia , Pneumocystis carinii/isolamento & purificação , Estações do Ano , Humanos , Incidência , Infecções por Pneumocystis/microbiologia , TemperaturaRESUMO
The determination of synergistic effects of antimicrobial drug combinations can lead to improved therapeutic options in the antibiotic treatment of cystic fibrosis patients who are chronically infected with multiresistant Pseudomonas aeruginosa isolates. The aim of this study was to evaluate the performance of the E test versus the standard agar dilution checkerboard susceptibility test in the assessment of synergy and, in addition, to determine the activity of two antimicrobial combinations against 163 multiresistant P. aeruginosa isolates from cystic fibrosis patients. The agreement between the checkerboard method and the E test was excellent (>90%) for nonmucoid as well as mucoid isolates from cystic fibrosis patients. The rate of synergy was higher for the antibiotic combination of ceftazidime and tobramycin (28.8% of the cystic fibrosis strains) than for the combination of meropenem and tobramycin (19.0%). However, the probability of synergy for the second antibiotic combination increased significantly when the synergy of the first antibiotic combination had already been demonstrated (Fischer's exact test, p=0.049). The results show that the E test is a valuable and practical method for routine microbiological diagnostics and can aid in the selection of improved antibiotic options in the treatment of cystic fibrosis patients chronically infected with P. aeruginosa.
