Primary isolation of Neisseria gonorrhoeae on hemoglobin-free New York City medium.

New York City medium and New York City medium without hemoglobin were comparatively evaluated for their ability to support the growth of Neisseria gonorrhoeae isolated from 1,010 clinical specimens. Although hemoglobin in the form of lysed horse erythrocytes stimulated gonococcal growth, the absence of this component from New York City medium did not have a detrimental effect on the recovery of gonococci isolated from clinical specimens. Both media were comparable in their ability to cultivate gonococci from clinical material, with a total of 187 gonococcal isolates being recovered on each of the media. The results of this study showed that the preparation of New York City medium can be facilitated and that its cost can perhaps be reduced by the elimination of the hemoglobin component from the formulation without adverse effect on the recovery of N. gonorrhoeae isolated from clinical specimens.

Use of New York City medium for improved recovery of Neisseria gonorrhoeae from clinical specimens.

New York City (NYC) and Martin-Lewis (ML) media were evaluated comparatively for their ability to support the growth of Neisseria gonorrhoeae from clinical specimens. A total of 1,010 urethral, cervical, pharyngeal, and rectal specimens were collected from walk-in patients attending a clinic for sexually transmitted diseases. A total of 187 and 165 isolates of gonococci were cultivated on NYC and ML media, respectively, with 161 of these isolates being recovered on both media. Overall, the use of NYC medium resulted in a 13.3% increased recovery rate of gonococci. When gonococci were recovered on both media from primary isolation, the NYC medium supported a more luxuriant growth and a greater number of colonies, which usually resulted in the detection of positive cultures 1 day sooner than on ML medium. Both media were comparable in their ability to suppress the growth of saprophytic microorganisms. The results of this study demonstrated that the use of NYC medium markedly enhanced the recovery of N. gonorrhoeae from clinical specimens as compared to ML medium.