RESUMO
Conductivity measurements in suspensions of biological cells have been used since many years for electroporation effectiveness evaluation. However, conductivity modeling by means of instantaneous values of current and voltage during pulse application does not take into account the effects of the sample reactance and the dielectric dispersion of the medium. This can lead to misinterpretation in the electroporation analysis. The electrical modeling and characterization of electroporation using sinusoidal signal analysis at 10 kHz proposed in this paper allows us to avoid distortions due to reactive effects of the sample. A simple equation establishes the relation between suspension conductivity and membrane conductance. This model was used in experiments with suspensions of yeast cells and applied electric fields of up to 450 kV/m for 1 ms. The analysis using the proposed model resulted in membrane conductance values of up to 8000 S/m (2) and allowed estimating the distribution profile of conductance on the cell membrane.
