RESUMO
To investigate the permeability of the dental enamel of erupted and unerupted human pre-molars and its variation with depth, we determined the resistivity of successive 100-micron-thick layers. The electrical resistance of halved tooth crowns at zero Hertz, which according to Scholberg et al. (1982, 1984) is inversely related to the permeability, was measured before and after removal of successive enamel layers from the outer surface toward the dentino-enamel junction (D.E.J.). The resistivity of the successive enamel layers increased from the D.E.J. toward the outer surface in approximately the same way in erupted as well as in unerupted pre-molars, except within the outermost layer of 100 to 200 microns thickness. The resistivity of this layer in erupted pre-molars was considerably higher than that in unerupted pre-molars, which may be due to post-eruptive mineralization.
Assuntos
Permeabilidade do Esmalte Dentário , Esmalte Dentário/ultraestrutura , Permeabilidade Dentária , Dente não Erupcionado/ultraestrutura , Adolescente , Dente Pré-Molar , Criança , Esmalte Dentário/metabolismo , Esmalte Dentário/fisiologia , Dentina/fisiologia , Dentina/ultraestrutura , Condutividade Elétrica , Eletroquímica , Humanos , Erupção Dentária , Dente não Erupcionado/metabolismo , Dente não Erupcionado/fisiologiaRESUMO
The permeability of dental enamel can be estimated by means of diffusion measurements with radio-isotopes. Because of the low intrinsic permeability of enamel membranes, determination via this route may take two weeks. A much faster electrochemical method is presented, in which the real and imaginary part of the membrane impedance are measured at discrete intervals in the frequency-range 1 Hz-1 MHz. Using the principles of network-analysis, a phenomenological interpretation of these electrochemical data in terms of a 6-parameter model is given. The model contains R0, the intrinsic membrane resistance, R infinity, the residual membrane resistance and the four parameters, necessary to describe the postulated enamel diffusion impedance, which bears strong resemblance to the Warburg impedance. Starting from this model, optimal estimates (with standard-errors) of a membrane's R0 and vmax (the log of the frequency where the imaginary part of the membrane impedance is maximal) were derived. These parameters were predictors of the permeability of enamel.
Assuntos
Esmalte Dentário/fisiologia , Permeabilidade Dentária , Animais , Bovinos , Membrana Celular/fisiologia , Difusão , Condutividade Elétrica , Modelos Biológicos , Fatores de TempoRESUMO
The proton binding behavior of the carbon monoxy derivatives of hemoglobins A and S, the respective beta-subunits (in the native form and with the cysteines combined with p-mercuribenzoate), and the respective beta (1-55) peptides have been measured. The results show that in the systems obtained from hemoglobin S there is a group which shifts its pK from about 7.0 to 8.35 in the beta-subunits that were reacted with p-mercuribenzoate and to more than 9.0 in the beta (1-55) peptide. Proton nuclear magnetic resonance measurements indicate that in the peptide beta (1-55) from hemoglobin S this residue is the histidine at beta 2. It is proposed that this pK shift is due to the formation of a salt bridge between beta 2 His and beta 7 Glu. This structure would disrupt the first turn of the A helix of the beta s-subunits. Its stabilization by extramolecular contacts may be relevant to the mechanism of fiber formation of hemoglobin S.