A Rhizocephalan Parasite Induces Pervasive Effects on Its Shrimp Host.

AbstractRhizocephalan barnacles are parasites of crustaceans that are known for dramatic effects on hosts, including parasitic castration, feminization, molt inhibition, and the facilitation of epibiosis. Most research on rhizocephalans has focused on carcinized hosts, with relatively little research directed to shrimp hosts that may experience distinct consequences of infection. Here, we describe a high-prevalence rhizocephalan-shrimp system in which multiple host changes are associated with infection: the dock shrimp Pandalus danae infected by the rhizocephalan Sylon hippolytes. In field-collected P. danae, infection by Sylon was associated with development of female sex characters at a smaller size and greater probability of epibiosis. Standardized video observations showed that infected P. danae performed grooming activities at higher rates than uninfected shrimp, suggesting that inhibited molting rather than direct behavioral modification is a likely mechanism for higher epibiosis rates. There was no difference in the composition of grooming behavior types or in general activity between infected and uninfected shrimp. Fatty acid compositions differed with infection, but total lipid concentrations did not, suggesting that parasite-driven shifts in host resource allocation were compensated or redirected from unmeasured tissues. Our results show that Sylon alters its host's role by provisioning an epibiotic substrate and also that it influences host physiology, resulting in feminization and fatty acid shifts. This study lays the groundwork for expanding rhizocephalan-shrimp research and encourages recognition of oft-ignored roles of parasitism in ecological communities.

Juvenile Dungeness crabs (Metacarcinus magister) selectively integrate and modify the fatty acids of their experimental diets.

Dungeness crabs (Metacarcinus magister) are ecologically and economically important in the coastal Northeast Pacific, yet relatively little is currently known about their feeding behaviour in the wild or their natural diet. Trophic biomarkers, such as fatty acids (FA), can be used to reveal trophic interactions. We used two feeding experiments to assess differences in FA composition of juvenile crabs fed different known foods to evaluate how they modify and integrate dietary FA into their own tissues and determine whether crab FA reflect diet changes over a six-week period. These experimental results were then compared with the FA signatures of wild caught juvenile crab with undetermined diets. We found that juvenile Dungeness crabs fed different foods assimilated dietary FA into their tissues and were distinct in their FA signatures when analysed with multivariate statistics. Experimentally fed juvenile crabs contained greater proportions of the most abundant long-chain polyunsaturated fatty acids (LCPUFA, >C20) than their foods. Crabs fed foods lacking in LCPUFA, particularly DHA (22:6ω3, docosahexaenoic acid), did not survive or grew slower than crabs fed other foods. This suggests that LCPUFA are physiologically important for this species and indicates biosynthesis of these FA does not occur or is not sufficient to meet their needs. This article is part of the theme issue 'The next horizons for lipids as 'trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.

The Purple Sea Urchin Strongylocentrotus purpuratus Demonstrates a Compartmentalization of Gut Bacterial Microbiota, Predictive Functional Attributes, and Taxonomic Co-Occurrence.

The sea urchin Strongylocentrotus purpuratus (order Camarodonta, family Strongylocentrotidae) can be found dominating low intertidal pool biomass on the southern coast of Oregon, USA. In this case study, three adult sea urchins were collected from their shared intertidal pool, and the bacteriome of their pharynx, gut tissue, and gut digesta, including their tide pool water and algae, was determined using targeted high-throughput sequencing (HTS) of the 16S rRNA genes and bioinformatics tools. Overall, the gut tissue demonstrated Arcobacter and Sulfurimonas (Epsilonproteobacteria) to be abundant, whereas the gut digesta was dominated by Psychromonas (Gammaproteobacteria), Propionigenium (Fusobacteria), and Flavobacteriales (Bacteroidetes). Alpha and beta diversity analyses indicated low species richness and distinct microbial communities comprising the gut tissue and digesta, while the pharynx tissue had higher richness, more closely resembling the water microbiota. Predicted functional profiles showed Kyoto Encyclopedia of Genes and Genomes (KEGG) Level-2 categories of energy metabolism, membrane transport, cell motility, and signal transduction in the gut tissue, and the gut digesta represented amino acid, carbohydrate, vitamin and cofactor metabolisms, and replication and repair. Co-occurrence network analysis showed the potential relationships and key taxa, such as the highly abundant Arcobacter and Propionigenium, influencing population patterns and taxonomic organization between the gut tissue and digesta. These results demonstrate a trend of microbial community integration, allocation, predicted metabolic roles, and taxonomic co-occurrence patterns in the S. purpuratus gut ecosystem.