RESUMO
Transcriptional activity of the human apolipoprotein (apo) A-II promoter has been reported in transiently transfected Caco-2 cells, but not in the intestine in vivo. In the present study we established that the transcription of a stably transfected reporter gene under the control of the -911/+29 human apo A-II, decreases with the onset of the differentiation process. This decrease paralleled that of the expression of the endogenous apo A-II gene. The decrease in apo A-II expression is also followed by a marked increase in the expression of the intestine-specific apo A-IV gene, analyzed here as a marker of enterocytic differentiation. Using clonal glucose metabolic variants of Caco-2 cells we have also observed that the lowest levels of apo A-II mRNA are associated with the lowest rates of glucose consumption. The illegitimate apo A-II transcriptional activity observed in Caco-2 cells is linked to the presence of DNase-I hypersensitive sites within the enhancer. This reflects a chromatin organization which allows, in Caco-2 cells as in the liver, the communication between the apo A-II enhancer and the proximal promoter, unlike what is observed in intestinal epithelial cells.
Assuntos
Apolipoproteína A-II/genética , Cromatina , Regulação da Expressão Gênica , Células CACO-2 , Desoxirribonuclease I , Humanos , Transcrição GênicaRESUMO
The culture of Caco-2 cells on plastic support impairs the expression of several genes involved in lipid metabolism. We describe culture conditions that permit the expression of the I-FABP gene and better expression of the apolipoprotein A-I, C-III, and A-IV genes. Basal lamina deposited on filters as well as the nature of nutrients on the apical side differentially modulated mRNA expression of I-FABP, APOBEC-1, and apolipoprotein genes. Growing cells on a filter led to functional polarization, illustrated by a secretion of apo B at the basal side, which induced the expression of the I-FABP, APOBEC-1, and apo A-IV genes and highly increased the expression of the apo C-III gene. Moreover, basal lamina deposited on the filter enhances the mRNA expression of apo A-I. Apo C-III and A-IV mRNA levels were decreased when cells were grown on a filter covered with basal lamina in the presence of a medium deprived of protein and lipid on the apical side, whereas these conditions had no effect on I-FABP, apo A-I, and APOBEC-1 mRNA levels. The addition of lipid micelles on the apical side had various effects, according to the genes. Caco-2 cells cultured under the conditions described here closely resembled enterocytes and represent a useful tool for studying the regulation of genes involved in lipid metabolism.