Pathway-Based Approaches for Assessing Biological Hazards of Complex Mixtures of Contaminants: A Case Study in the Maumee River.

Assessment of ecological risks of chemicals in the field usually involves complex mixtures of known and unknown compounds. We describe the use of pathway-based chemical and biological approaches to assess the risk of chemical mixtures in the Maumee River (OH, USA), which receives a variety of agricultural and urban inputs. Fathead minnows (Pimephales promelas) were deployed in cages for 4 d at a gradient of sites along the river and adjoining tributaries in 2012 and during 2 periods (April and June) in 2016, in conjunction with an automated system to collect composite water samples. More than 100 industrial chemicals, pharmaceuticals, and pesticides were detected in water at some of the study sites, with the greatest number typically found near domestic wastewater treatment plants. In 2016, there was an increase in concentrations of several herbicides from April to June at upstream agricultural sites. A comparison of chemical concentrations in site water with single chemical data from vitro high-throughput screening (HTS) assays suggested the potential for perturbation of multiple biological pathways, including several associated with induction or inhibition of different cytochrome P450 (CYP) isozymes. This was consistent with direct effects of water extracts in an HTS assay and induction of hepatic CYPs in caged fish. Targeted in vitro assays and measurements in the caged fish suggested minimal effects on endocrine function (e.g., estrogenicity). A nontargeted mass spectroscopy-based analysis suggested that hepatic endogenous metabolite profiles in caged fish covaried strongly with the occurrence of pesticides and pesticide degradates. These studies demonstrate the application of an integrated suite of measurements to help understand the effects of complex chemical mixtures in the field. Environ Toxicol Chem 2021;40:1098-1122. © 2020 SETAC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

Behavior of immunologically castrated barrows in comparison to gilts, physically castrated barrows, and intact male pigs.

The behavior of immunologically castrated barrows (IC; with Improvest; Zoetis, Parsippany, NJ) was compared with that of intact males (IM), physically castrated barrows (PC), and gilts (G). The study used 160 commercial crossbred pigs in a randomized complete block design (blocking factor was start date of study) and was performed over an 8-wk period from 16 wk of age (67.2 ± 2.50 kg BW) to a final BW of 126.5 ± 6.05 kg. The first Improvest dose was given to IC at the start of the study (d 0), and the second dose was given 28 d later. Pigs were housed in single-gender groups of 4 and had ad libitum access to feed and water. General, aggressive, and sexual behaviors were observed over a 12-h period from 0600 to 1800 h by the same trained investigators on d -1 (d prior to first dose), 13, 27 (day prior to second dose), 34, 41, and 55 of study (end of test). General behaviors (number of pigs lying, sitting, standing, at feeder, and at drinker) were recorded every 10 min in all pens (10 pens/gender), and aggressive (bites, head butts, and fights) and sexual (mounts) behaviors were recorded continuously over the 12-h period on a subsample of 4 pens/gender. There was no difference ( > 0.05) between the genders for lying, sitting, or drinker-related behavior. For the 4-wk period prior to second dose, a greater percentage of PC and G were at the feeder ( < 0.05) than IC or IM (8.0%, 7.4%, 10.2%, and 9.3% for IC, IM, PC, and G, respectively; SEM = 0.44). However, after the second dose, the percentage of pigs at the feeder was similar ( > 0.05) for IC, PC, and G and was greater ( < 0.05) for those genders than IM (10.1%, 7.1%, 10.0%, and 8.8%, respectively; SEM = 0.50). Prior to the second dose, the incidence of aggressive behaviors was generally greater for IC and IM compared with PC and G; however, after the second dose, the incidence of aggressive behaviors was similar for IC and PC and lower ( < 0.05) for those genders than for IM. The frequency of mounts for IC was greater ( < 0.05) than for PC before (25.5, 27.3, 2.5, and 1.5 total mounts/pen, respectively; SEM = 4.37) but not after ( > 0.05; 3.3, 33.3, 0.25, and 0.25 total mounts/pen, respectively; SEM = 1.15) the second dose. These results suggest that prior to the second Improvest dose, the behavior of IC was similar to that of IM and transitioned to become more similar to the behavior of PC after the second dose.

A summary review of carcass cutability data comparing primal value of immunologically and physically castrated barrows.

The objectives were to 1) assess cutability, quality, and value of carcasses from immunologically castrated (IC) barrows compared with carcasses from physically castrated (PC) barrows and 2) evaluate the effect of hot carcass weight (HCW) on cutability and value of IC barrows summarizing U.S. data. Lean cutting yield (LCY) was defined as: LCY = [(whole ham + trimmed loin + Boston butt + picnic + spareribs)/chilled side wt] x 100. Carcass cutting yield (CCY) was determined using the following equation: CCY = [(lean cutting yield components + natural fall belly)/chilled side wt] x 100. To evaluate the effects of HCW of IC barrows on carcass cutting yields, IC barrows were grouped by HCW: light ( < 90.9 kg), average (90.9-97.7 kg), or heavy ( > 97.7 kg). Differences in the value of the carcass components for IC and PC barrow carcasses were calculated using a 5 yr average of meat prices from the USDA Agriculture Marketing Service and the carcass cutting yield estimates generated from this summary. Data were analyzed using the MIXED procedure of SAS with fixed effects of Improvest treatment or HCW group. Study was included as a random effect. This review allowed for a summarization of the treatment averages of 851 IC and PC barrow carcasses. Lean cutting yield of IC barrows was 1.41 units greater (P < 0.0001) than PC barrows (70.97 vs. 69.56%). Similarly, CCY of IC barrows was 1.29 units greater (P < 0.001) compared with PC barrows (87.27 vs. 85.98%). As HCW of IC barrows increased, both CCY and LCY declined (P < 0.01), with light IC barrow carcasses having a 1.43 unit advantage in CCY compared with heavy IC barrow carcasses (P < 0.01). Natural fall bellies of PC barrows comprised a greater (P < 0.05) percentage of side weight than those from IC barrows (15.81 vs. 15.50%). A reduction in belly primal value was confirmed by a 3.43 unit reduction in the commercial bacon slicing yields of IC barrows. However, belly yield and slicing yield differences were minimized when IC barrows were marketed at a heavier weight. Using carcass cutout estimates determined in the summary as the foundation for value calculations, lean cuts of IC barrow carcasses were worth $2.66 to $3.80 more than PC barrow carcasses. Therefore, after adjustment for the reduction in belly primal value, the primal value of an IC barrow carcass was $2.08 to $3.13 greater than a PC barrow carcass.

Ham and belly processing characteristics of immunological castrated barrows (Improvest) fed ractopamine hydrochloride (Paylean).

Effects of sex class (physically castrated, PC or immunologically castrated, IC) and diet (0 or 5mg/kg ractopamine hydrochloride, RAC) on characteristics of ham and bellies were determined from pigs slaughtered in three groups with similar ending live weights. One carcass per pen per marketing group (n=8) was selected to evaluate further processing characteristics. Data were analyzed as a 2×2 factorial design with a split plot in time and fixed effects of sex, diet, marketing group, and their interactions. IC fresh bellies were thinner (P<0.01) and softer (P<0.01) than PC bellies. IC hams and bellies were leaner (P<0.05) than those from PC pigs. RAC feeding did not affect (P>0.05) fresh ham or belly characteristics but decreased (P<0.01) fat in cured PC bellies. Marketing group affected (P<0.05) fresh quality, processing characteristics, and composition of hams and bellies. Immunological castration and RAC produced leaner finished products but did not alter processing yield of hams or bacon.

Evaluation of whole-mount in situ hybridization as a tool for pathway-based toxicological research with early-life stage fathead minnows.

Early-life stage fish can be more sensitive to toxicants than adults, so delineating mechanisms of perturbation of biological pathways by chemicals during this life stage is crucial. Whole-mount in situ hybridization (WISH) paired with quantitative real-time polymerase chain reaction (QPCR) assays can enhance pathway-based analyses through determination of specific tissues where changes in gene expression are occurring. While WISH has frequently been used in zebrafish (Danio rerio), this technology has not previously been applied to fathead minnows (Pimephales promelas), another well-established small fish model species. The objective of the present study was to adapt WISH to fathead minnow embryos and larvae, and use the approach to evaluate the effects of estrone, an environmentally-relevant estrogen receptor (ER) agonist. Embryos were exposed via the water to 0, 18 or 1800 ng estrone/L (0, 0.067 and 6.7nM) for 3 or 6 days in a solvent-free, flow-through test system. Relative transcript abundance of three estrogen-responsive genes, estrogen receptor-α (esr1), cytochrome P450-aromatase B (cyp19b), and vitellogenin (vtg) was examined in pooled whole embryos using QPCR, and the spatial distribution of up-regulated gene transcripts was examined in individual fish using WISH. After 3 days of exposure to 1800 ng estrone/L, esr1 and cyp19b were significantly up-regulated, while vtg mRNA expression was not affected. After 6 days of exposure to 1800 ng estrone/L, transcripts for all three genes were significantly up-regulated. Corresponding WISH assays revealed spatial distribution of esr1 and vtg in the liver region, an observation consistent with activation of the hepatic ER. This study clearly demonstrates the potential utility of WISH, in conjunction with QPCR, to examine the mechanistic basis of the effects of toxicants on early-life stage fathead minnows.

Color stability and sensory characteristics of fresh and enhanced pork loins from immunologically castrated barrows.

Our objective was to evaluate color changes during storage of fresh pork chops and enhanced and nonenhanced loin quality from anti-gonadotropin-releasing factor (Improvest) immunologically castrated (IC) barrows. In study 1, treatments evaluated were IC barrows, IC barrows fed ractopamine (IC+RAC), physically castrated (PC) barrows, intact males (IM), and gilts. Fresh loins were evaluated for sensory characteristics and instrumental tenderness including both Warner-Bratzler Shear force (WBSF) and star probe force. In study 2, treatments evaluated were IC barrows fed 0.55% and 0.65% standard ileal digestible (SID) lysine and PC barrows fed 0.55% SID lysine. Loin chops were displayed for 7 d, and color was evaluated. The remaining loin was halved, and one half was enhanced with a 3.5% salt and phosphate solution. Enhanced loins were evaluated for sensory characteristics and instrumental tenderness. In both studies, pen served as the experimental unit for all traits measured. Data from individual animals were averaged by pen and analyzed, per study, as a 1-way ANOVA using the MIXED procedure of SAS. In study 1, there were no differences ( 0.05) between treatments for juiciness, tenderness, chewiness, or off-flavor. Intact males had the most intense ( 0.05) boar aroma. Gilts had the most intense ( < 0.05) pork flavor, whereas IM had the least intense pork flavor; all other treatments were intermediate. Tenderness (WBSF) was similar between treatments at 1 d of aging; however, at 7, 14, and 21 d of aging, loins from IC barrows were more tender ( 0.05) than those from gilts, IM, and IC+RAC but were similar ( 0.05) to those from PC. In study 2, discoloration of fresh loin chops during storage was similar ( 0.42) between PC and IC barrows. Chops from enhanced loins were more tender and juicy but had more off-flavor than nonenhanced loins ( 0.01). Star probe and WBSF were also reduced ( 0.01) in enhanced loins compared with nonenhanced loins. Sensory characteristics and tenderness were not different between treatments of IC and PC barrows ( > 0.05), and there were no interactions of enhancement with castration treatments. These data suggest that immunological castration does not negatively impact the color stability, sensory characteristics, or tenderness of enhanced or nonenhanced pork loins.

A comparison of slice characteristics and sensory characteristics of bacon from immunologically castrated barrows with bacon from physically castrated barrows, boars, and gilts.

The objectives were to compare slice characteristics and sensory attributes of bacon from immunologically castrated (IC) barrows with bacon from other sexes using a trained sensory panel. Bacon was obtained for sensory evaluation from 3 experiments. In Exp. 1, trimmed and squared bellies (n=180) of IC barrows, IC barrows fed ractopamine hydrochloride (IC+RAC), physically castrated (PC) barrows, intact males (IM), and gilts were used. Data were analyzed as a general linear mixed model and pen (n=48) served as the experimental unit. Treatment (sex or diet) was a fixed effect in all 3 experiments. In Exp. 2, untrimmed, natural fall bellies (n=96) from IC and PC barrows fed 0 or 30% or a withdrawal distillers dried grains with solubles (DDGS) program when slaughtered at 5 wk after the second dose (25 wk of age) were used. In Exp. 3, untrimmed, natural fall bellies (n=96) from IC and PC barrows fed the same experimental diets as in experiment 2 but slaughtered at 7 wk after the second dose (27 wk of age) were used. Data from Exp. 2 and 3 were analyzed as a 2×3 factorial arrangement in a randomized complete block design and pen was the experimental unit. Bellies from all 3 experiments were processed using the same protocols. In Exp. 1, IM had the greatest (P<0.0001) boar aroma and flavor scores among all treatments. No differences were detected among the other treatment groups for boar aroma or flavor. Intact males had the least (P<0.0001) desirable cured bacon aroma and flavor among all treatments. No differences were detected among the other treatment groups for bacon aroma or flavor. There were no differences in bacon aroma or off-flavor between IC and PC barrows slaughtered at 5 wk after the second dose regardless of DDGS feeding program. Bacon from PC barrows was saltier (P<0.01) than bacon from IC barrows when slaughtered at 5 wk after the second dose. There were no differences in bacon aroma, off-aroma, bacon flavor, or saltiness between IC and PC barrows slaughtered at 7 wk after the second dose regardless of DDGS feeding program. Total slice area of bacon slices from IC barrows slaughtered at 5 wk after the second dose were less (P<0.01) than PC barrows, but the differences diminished (P=0.16) when slaughtered at 7 wk after the second dose. Overall, panelists successfully detected boar odor and flavor in Exp. 1. Immunological castration was as effective as physical castration at eliminating boar aroma and boar flavor in bacon even when feeding differing DDGS feeding programs or when slaughtered at 5 or 7 wk after the second dose.

Effects of ractopamine on growth performance and carcass characteristics of immunologically and physically castrated barrows and gilts.

The study was performed to evaluate the effect of feeding ractopamine (RAC) to physically castrated barrows (PC), immunologically castrated barrows (IC), and gilts (gilts) using a randomized complete block design with a 3 × 3 factorial arrangement of treatments: 1) sex (PC, IC, and gilts) and 2) RAC inclusion level (0, 5, and 7.5 mg/kg). The IC received 2 doses of gonadotropin releasing factor analog-diphtheria toxoid conjugate (Improvest; Zoetis, Kalamazoo, MI) at the start of the study (wk 16 of age; 69.6 ± 2.96 kg BW) and 4 wk later. The study used 180 pigs housed in groups of 4 (5 groups/sex × RAC subclass) and was performed over a fixed time of 61 d with RAC being fed for the final 26 d of study. Diets were formulated to meet requirements of intact males for the first 35 d and of intact males fed 7.5 mg/kg RAC for the remainder of the study. Pigs had ad libitum access to feed and water throughout the study period. At the end of the study, pigs were harvested at a commercial facility and HCW and last rib backfat thickness were measured. There were no treatment interactions (P > 0.05) for any variables. For the overall study period, IC had greater (P ≤ 0.05) overall ADG compared to PC, which grew faster (P ≤ 0.05) than gilts (1,246, 1,083, and 1,025 g/d for IC, PC, and gilts, respectively; SEM = 20.3); ADFI was lower (P ≤ 0.05) for gilts than IC and PC, which had similar ADFI (3.36, 3.37, and 2.87 kg/d, respectively; SEM = 0.051); and G:F was greater (P ≤ 0.05) for IC than gilts and greater for gilts than PC (0.371, 0.322, and 0.358 kg/kg, respectively; SEM = 0.0039). For the period from the second dose to the end of study, IC had greater (P ≤ 0.05) ADG (28.6%), ADFI (12.3%), and G:F (14.3%) than PC. Carcass yield was lower (P ≤ 0.05) for IC compared to PC and gilts (72.8, 75.0, and 74.6%, respectively; SEM = 0.25). Feeding RAC increased (P ≤ 0.05) ADG (15.7 and 14.5% for 5 and 7.5 mg/kg, respectively), G:F (17.1 and 16.4%, respectively), carcass weight (3.7 and 3.2 kg, respectively), and carcass yield (1.0 and 1.0 percentage unit, respectively) compared to the control. These results highlight sex differences in and effects of RAC on growth and carcass characteristics and suggest that the relative response to RAC is similar in IC and PC.

Effect of gonadotropin releasing factor suppression with an immunological on growth performance, estrus activity, carcass characteristics, and meat quality of market gilts.

Objectives were to evaluate the administration of an anti-gonadotropin releasing factor (GnRF) analog on suppression of estrus, consistency of feed intake, and growth performance in market gilts and to investigate the impact the physiological changes would have on carcass characteristics and fresh meat quality. Gonadotropin releasing factor stimulates the anterior pituitary to release luteinizing hormone that acts on the ovary to induce follicle development and indirectly initiates ovulation. Improvest (Zoetis, Kalamazoo, MI) contains an incomplete version of naturally occurring GnRF and causes the production of anti-GnRF antibodies that bind to the GnRF receptor and thus render GnRF inactive. This in turn suppresses estrus in female pigs. Gilts were initially separated into 10 blocks based on age and then within each block allotted to a pen (n = 114; 5 pigs/pen) based on BW. Gilts received the first dose at 12 wk of age and the second dose at 16 wk of age, were exposed to a boar daily from 20 to 26 wk of age, and were slaughtered at 26 wk of age (10 wk after second dose). Meat quality was analyzed on the 2 gilts closest to pen average ending live weight in 5 of the 10 blocks. Pen served as the experimental unit for all data analysis. During the 15-wk finishing period, ADG was 0.03 kg greater (P < 0.01) and G:F was 0.009 greater (P = 0.02) in gilts administered GnRF suppression (treated) compared with untreated gilts (control). The majority of improvements in growth performance were observed from 16 to 20 wk of age (4 wk after second dose), as ADG was 0.07 kg greater (P < 0.001) and G:F was 0.021 greater (P < 0.01) in treated gilts compared with control gilts. Ovarian weights were reduced (P < 0.0001) by 64.15% and gilts exhibiting puberty were reduced by 87.80% (P < 0.001) in treated gilts compared with control gilts. Back fat depth was 3.78 mm greater (P < 0.0001) and estimated lean was 1.31 percentage units less (P < 0.0001) in treated gilts compared with control gilts. With the exception of subjective color, there were no differences (P ≥ 0.12) in meat quality parameters between treated and control gilts. Subjective color was darker (P = 0.03) in treated gilts compared with control gilts. These data suggest market gilts treated with an anti-GnRF analog had suppressed estrus and episodical changes in ADFI, while they had improved feed efficiency, increased ADG, and increased back fat depth when compared with gilts without an anti-GnRF analog treatment.

Effects of immunological castration (Improvest) on further processed belly characteristics and commercial bacon slicing yields of finishing pigs.

Objectives were to compare fresh belly characteristics, further processed belly characteristics, and commercial bacon slicing yields of immunologically castrated (IC) barrows, IC barrows fed ractopamine hydrochloride (IC+RAC), physically castrated (PC) barrows, intact males, and gilts. One hundred eighty-eight bellies from pigs housed in single sex pens (n = 48) slaughtered at 130 kg ending live weight were evaluated for flop distance, length, width, thickness, and fatty acid composition. Bellies were injected, thermally processed, and sliced according to standard protocols at a USDA federally inspected facility. Complete slices were sorted by trained plant personnel. Then, sliced bellies were individually packaged to maintain anatomical orientation. The effects of treatments were analyzed as a generalized linear mixed model with pen of pigs serving as the experimental unit for all comparisons. Belly thickness was not different (P ≥ 0.11) in bellies from IC barrows (3.74 cm) compared with bellies from IC+RAC (3.60 cm), PC barrows (3.94 cm), or gilts (3.64 cm); however, bellies were 0.42 cm thicker (P < 0.01) in IC barrows compared with intact males. Iodine value was not different (P ≥ 0.06) in bellies from IC barrows (68.26) compared with bellies from IC+RAC (69.78), PC barrows (67.55), or gilts (69.45); however, iodine value was 2.32 units less (P < 0.01) in IC barrows compared with intact males. Green weight was not different (P ≥ 0.09) in bellies from IC barrows (4.88 kg) compared with bellies from IC+RAC (5.11 kg), PC barrows (5.10 kg), or gilts (4.79 kg); however, green weight was 0.56 kg greater (P < 0.0001) in IC barrows compared with intact males. Pump uptake and cooked yield was not different (P ≥ 0.15) among treatment groups. Slicing yield (as a percentage of green weight) was decreased (P ≤ 0.01) in bellies from IC barrows (93.61%) compared with bellies from PC barrows (98.42%) or gilts (98.19%); however, slicing yield was not different (P ≥ 0.11) among bellies from IC barrows, IC+RAC, or intact males. Overall, fresh belly characteristics of IC barrows were analogous to PC barrows and gilts, but commercial slicing yields of bacon manufactured from IC barrows were less than both PC barrows and gilts.

Effects of feeding ractopamine hydrochloride (Paylean) to physical and immunological castrates (Improvest) in a commercial setting on carcass cutting yields and loin quality.

Effects of feeding ractopamine (RAC; 5 mg/kg) to physically castrated (PC) and immunologically castrated (IC) pigs on carcass characteristics, cutting yields, and loin quality were evaluated using 285 carcasses. Male pigs were randomly assigned to sex treatments (PC and IC) at birth and fed the same nursery diets before allotment into 32 pens with 22 pigs per pen in a grow-finish barn. Pigs in the PC group were physically castrated at approximately 5 d of age, and pigs in the IC group were administered Improvest at 11 and 18 wk of age. Diet treatments (control or RAC) were initiated on study d 87. Pigs were marketed at 12 d (4.5 wk post-second Improvest dose), 19 d (5.5 wk post-second Improvest dose), and 33 d (7.5 wk post-second Improvest dose) following the start of final diet treatments. Three carcasses per pen were selected for evaluation of cutting yields and loin quality. Data were analyzed using PROC MIXED in SAS with fixed effects of sex, diet, market group, and their interaction; carcass (N = 285) was the experimental unit. Carcasses from RAC-fed pigs were heavier (P < 0.01) and had deeper (P = 0.02) loins than control-fed carcasses. Carcasses from IC pigs were similar (P = 0.22) in weight but had less (P < 0.01) fat and shallower (P = 0.02) loins when compared to PC carcasses. There were differences (P < 0.05) among market groups for carcass weights, fat depths, loin depths, and estimated carcass leanness. For cutting yields, RAC-fed carcasses had greater (P ≤ 0.03) bone-in lean and total carcass cutting yields than control-fed carcasses while there were no differences (P > 0.05) between RAC-fed and control-fed carcasses when evaluating LM color, marbling, firmness, pH, drip loss, and tenderness. Carcasses from IC pigs had greater (P < 0.05) boneless lean yields, bone-in lean yields, and total carcass cutting yields than PC carcasses. There were minimal differences (P < 0.05) in LM marbling, firmness, composition, and tenderness between PC and IC pigs. There was an interaction (P = 0.03) between sex and diet for LM composition. Control-fed PC loins had more (P < 0.01) lipid than all other treatment combinations. Market group had effects (P < 0.05) on carcass cutting yields, LM color, marbling and firmness scores, pH, purge loss, composition, and tenderness. The results from this study indicated RAC and immunological castration were additive in terms of improving carcass cutting yields while having minimal effects on pork quality.

Effects of immunocastration and time after second Improvest dose on adipose tissue fatty acid profile of finishing barrows.

The effects of immunocastration and time after second Improvest dose on proximate composition and fatty acid profile of adipose tissue from jowl and belly of finishing barrows were determined. Physically castrated (PC) and immunologically castrated (IC) barrows were assigned to treatments at birth. Within 5 d of age, PC barrows were physically castrated and IC barrows were administered Improvest (gonadotropin-releasing factor analog; Zoetis, Kalamazoo, MI) at 16 and 20 wk of age. Diets were formulated with corn and soybean meal and did not contain ethanol coproducts. Subsequently, PC (n = 23) and IC (n = 24) barrows were slaughtered biweekly from 22 to 28 wk of age, 2 to 8 wk following second Improvest dose. Adipose tissue samples were collected from the jowl and belly. Main effects of castration method and time after second Improvest dose and their interaction were analyzed with the MIXED procedure of SAS. Overall, IC barrows were heavier at slaughter (136.1 vs. 100.6 kg; P < 0.01), had similar HCW (P = 0.13), and were leaner (50.9 vs. 48.9%; P < 0.01) than PC barrows. In jowl adipose tissue, IC barrows had less lipid content (64.7 vs. 80.2%; P < 0.01) than PC barrows at 2 wk. However, there were no differences (P ≥ 0.75) in lipid content between IC and PC barrows from 4 to 8 wk. Total MUFA content was reduced (42.5 vs. 44.8%; P = 0.04) and PUFA content was greater (16.9 vs. 14.8%; P < 0.01) in IC compared to PC barrows. Increasing time after second dose reduced (P = 0.04) PUFA content from 17.5% at 2 wk to 15.4% at 8 wk. In IC barrows, iodine value (IV) was increased by 6.9 and 3.3 g/100 g compared with PC barrows at 2 and 4 wk, but IV was not different between castration methods (P ≥ 0.95) at both 6 and 8 wk. Meanwhile, in belly adipose tissue, IC barrows had less lipid content than PC barrows (75.3 vs. 82.7%; P < 0.01). Lipid content was unchanged over time (P = 0.34) in either PC or IC barrows. Total SFA and MUFA content of the belly were similar for both IC and PC barrows. Belly adipose tissue from IC barrows had greater concentrations of PUFA than PC barrows (16.2 vs. 14.7%; P < 0.01). In IC barrows, IV was increased by 5.3 g/100 g compared with PC barrows at 2 wk, but IV was not different between castration methods (P ≥ 0.91) from 4 to 8 wk. Results indicate that as time after second Improvest dose progressed, IV of jowl and belly was reduced. This was accompanied by an increase in lipid deposition in jowl, while lipid content remained constant in belly.

Effects of feeding ractopamine hydrochloride (Paylean) to physical and immunological castrates (Improvest) in a commercial setting on growth performance and carcass characteristics.

Growth performance and carcass characteristics of physically castrated (PC) and immunologically castrated (IC) pigs fed ractopamine hydrochloride (RAC; 5 mg/kg) were evaluated in 64 pens of 22 pigs each. Male pigs were randomly assigned to castration method at birth. Pigs in the PC group were physically castrated at 5 d of age while IC pigs were administered Improvest at 11 and 18 wk of age. Pigs entered the grow-finish barn at approximately 9 wk of age (d 0). Dietary treatments (control or RAC) were initiated on d 87. Final treatment arrangement was a 2 × 2 factorial of castration method and diet. Data were analyzed using a mixed model with fixed effects of castration method, diet, market group, and all 2- and 3-way interactions. Pen was the experimental unit. From d 0 to 65, IC pigs had 11.2% greater (P < 0.01) G:F and 11.6% less (P < 0.01) ADFI than PC pigs, but ADG was increased 1.0% in PC pigs compared with IC pigs (P < 0.01). From d 65 to 87, IC pigs had 7.9% greater (P < 0.01) ADG and 12.1% greater (P < 0.01) G:F than PC pigs while having similar (P = 0.16) ADFI. At the initiation of diet (RAC) treatments, BW of all treatments were similar (P ≥ 0.32). From d 87 to 120 (RAC feeding period), IC pigs had 10.0% greater (P < 0.01) ADG and 10.5% greater (P < 0.01) ADFI than PC pigs while having similar (P = 0.64) G:F. Feeding RAC increased (P < 0.01) ADG by 16.9% and G:F by 17.9% while having no effect (P = 0.42) on ADFI from d 87 to 120. There were no significant interactions between castration method and diet on growth performance from d 87 to 120. For the entire study (d 0-120), IC pigs had 2.6% greater (P < 0.01) ADG, 4.6% less (P < 0.01) ADFI, and 7.3% greater (P < 0.01) G:F than PC pigs. Averaged over market groups, IC pigs were 2.5 kg heavier (P < 0.01) and had similar (P = 0.10) carcass weights and 1.8 percentage units less (P < 0.01) dressing yields than PC pigs. Additionally, IC pigs had 1.3 mm less (P < 0.01) fat and 1.7 mm less (P < 0.01) loin depth than PC pigs. Pigs fed RAC were 2.9 kg heavier (P < 0.01) and had 2.3 kg heavier (P < 0.01) carcasses and 2.2 mm deeper (P < 0.01) loins but similar (P = 0.21) dressing yields and tended (P < 0.10) to have 0.4 mm less fat than control-fed pigs when averaged over market groups. Group 3 pigs were the heaviest (P < 0.01) at slaughter and had the heaviest (P < 0.01) carcasses, greatest (P < 0.01) dressing yields, and the most (P < 0.01) carcass fat of all market groups. Overall, immunological castration and RAC were additive in terms of improving growth performance and carcass characteristics.

Effects of immunological castration and distiller's dried grains with solubles on carcass cutability and commercial bacon slicing yields of barrows slaughtered at two time points.

Male pigs were randomly assigned to a castration method at birth and allotted to 48 pens (28 pigs/pen). Physically castrated (PC) barrows were castrated at 2 d of age; immunologically castrated (IC) barrows were administered Improvest (GnRF analog diphtheria toxoid conjugate; Zoetis, Kalamazoo, MI) at 16 and 20 wk of age. Distiller's dried grains with solubles (DDGS) feeding strategies included either 0% DDGS (control), 30% DDGS (30% DDGS) fed from 6 wk of age to slaughter, or 30% DDGS fed from 6 wk of age to second dose of Improvest and then fed 0% DDGS until slaughter (withdrawal). Four barrows closest to the median pen weight at 4.5 wk after second dose were selected for evaluation; two were randomly selected and slaughtered at 5 wk and the other two at 7 wk after second dose. Data from each slaughter time were analyzed independently as a 2 × 3 factorial design with pen as the experimental unit. At 5 wk after second dose, bone-in lean cutting yields were 2.63% units greater (P < 0.01) in IC when compared to PC. Bellies were thicker (P < 0.01) and tended to have greater belly flop distances (P = 0.07) in PC compared to IC, however iodine values (IV) were not altered (P = 0.84). Carcass traits (P ≥ 0.10), cutting yields (P ≥ 0.43), and fresh belly characteristics (P ≥ 0.08) were minimally affected by DDGS feeding strategy. Bacon slicing yields (percentage of green weight) were 6.10% units less (P < 0.01) in IC compared with PC. At 7 wk after second dose, bone-in lean cutting yields were 1.57% units greater (P = 0.03) in IC compared with PC. Distiller's grains feeding strategy had no effect (P ≥ 0.83) on boneless carcass cutting yields in IC; while in PC, these yields were 2.32% units less (P < 0.02) in control-fed barrows when compared to other feeding strategies (castration method × feeding strategy; P = 0.03). Bellies from PC tended to be thicker (P = 0.07) and have similar flop distances (P = 0.44) and IV (P = 0.54) when compared with IC. Iodine value was greater (P = 0.03) in 30% DDGS-fed barrows compared with control-fed barrows. Bacon slicing yields (percentage of green weight) were 4.27% units less (P = 0.05) in IC compared with PC. These data suggested that while bacon slicing yield was reduced in IC barrows fed control and 30% DDGS compared with PC barrow counterparts, withdrawal of DDGS improved bacon slicing yields of IC barrows.

Growth performance of immunologically castrated (with Improvest) barrows (with or without ractopamine) compared to gilt, physically castrated barrow, and intact male pigs.

The study used a randomized complete block design (blocking factor was date of start on test) with 5 treatments: 1) physically castrated barrows (PC), 2) intact males (IM), 3) gilts (G), 4) immunologically castrated barrows (IC), and 5) immunologically castrated barrows fed ractopamine at 5 mg/kg (IC+RAC). The study used 192 pigs and was performed from the 16 wk of age (67.2 ± 2.52 kg BW) to a pen mean BW of 132.5 ± 3.60 kg. For IC+RAC, ractopamine was fed for the final 23 d of the study. Pigs were housed in groups of 4 (10 groups for PC, IM, G, and IC and 8 groups for IC+RAC) in a finishing building at a floor space of 1.18 m(2)/pig. Diets were formulated to meet requirements of IM except that the diet for the IC+RAC fed during the ractopamine feeding period was formulated to meet requirements of pigs on that treatment. Pigs had ad libitum access to feed and water throughout the study period and were individually weighed at the start, wk 2 and 4, and subsequently every week until the end of study. For the overall study period, IC had greater (P ≤ 0.05) ADG than the other genders (1,150, 1,024, 1,064, and 954 g/d for IC, PC, IM, and G, respectively; SEM = 25.8) and required fewer days to reach slaughter weight than the other genders (58.1, 61.6, 61.6, and 66.5 d for IC, PC, IM, and G, respectively; SEM = 1.26). Overall ADFI was less (P ≤ 0.05) for IM and G than IC and PC, which were similar (P > 0.05) in this respect (3.11, 3.06, 2.68, and 2.75 kg/d for IC, PC, IM, and G, respectively; SEM = 0.061). Overall G:F was greater (P ≤ 0.05) for IM than the other genders; IC had greater overall G:F than PC and G, which were similar in this respect (0.371, 0.335, 0.397, and 0.347 kg/kg for IC, PC, IM, and G, respectively; SEM = 0.0068). Immunologically castrated barrows had greater (P ≤ 0.05) ADG (30.7%) and ADFI (22.5%) than PC from the second week following the second Improvest dose to the end of the study. During the ractopamine feeding period, IC+RAC had greater (P ≤ 0.05) ADG (11.6%) and G:F (17.3%) than IC. The results of this study confirmed previously observed gender differences and effects of ractopamine on growth performance and that IC grew faster and had greater feed efficiency than PC during the study period.

The effects of immunological castration and corn dried distillers grains with solubles withdrawal on growth performance, carcass characteristics, fatty acid analysis, and iodine value of pork fat depots.

A total of 1,360 pigs were used in a 125-d study to determine the effects of corn dried distillers grains with solubles (DDGS) withdrawal after immunological castration (Improvest, Zoetis, Kalamazoo, MI) on growth performance and carcass fat quality of pigs. Pens of male pigs (initially 24 kg) were randomly allotted by BW and castration method (physically castrated [PC] or immunologically castrated [IC] barrows) to 1 of 3 diets with 8 replications per treatment and 27 to 29 pigs per pen. Treatments were arranged in a 2 × 3 factorial with main effects of castration method and diet (0% DDGS throughout, 30% DDGS throughout, or 30% DDGS through d 75 then no DDGS to d 125). Intact males were injected with Improvest on d 39 and 74 (IC). No castration method × diet interactions (P > 0.12) were observed for growth performance. Before the second Improvest injection (d 0 to 74), PC barrows had increased (P < 0.05) ADFI but were less efficient (P < 0.05) than intact males. After the second Improvest injection until the first marketing event (d 74 to 107), IC barrows had improved (P < 0.05) ADG and G:F compared with PC barrows. From d 0 to 107, IC barrows had improved (P < 0.05) ADG, G:F, and lower ADFI than PC barrows. The inclusion of 30% DDGS decreased (P < 0.05) G:F compared with pigs fed the control diet. For the period after the second Improvest injection (d 74 to 125), IC barrows had increased (P < 0.05) ADG, ADFI, and G:F compared with PC barrows. Overall (d 0 to 125), IC barrows had improved (P < 0.05) ADG and G:F and lower ADFI than PC barrows. The inclusion of 30% DDGS decreased (P < 0.05) G:F. Carcass yield was lower (P < 0.05) for IC than PC barrows. Pigs fed 30% DDGS throughout had decreased (P < 0.05) carcass yield; however, withdrawing DDGS from the diet on d 74 was effective at fully recovering the yield loss. Carcass fat iodine values (IV) were consistently higher (P < 0.05), regardless of fat depot or harvest time when 30% DDGS were included in the diet. Multiple 2-way interactions (P < 0.05) were detected between castration method, DDGS, depot, and time. Interactions were a result of fatty acid profiles changing more rapidly in backfat and belly fat than in jowl fat from d 107 to 125 and more dramatically in IC than PC barrows in the same period. This improvement from d 107 to 125 could be caused by the dilution of unsaturated fatty acids, specifically C18:2 and C18:3, due to rapid deposition of fat from de novo synthesis in IC barrows.

Effects of immunological castration (Improvest) on changes in dressing percentage and carcass characteristics of finishing pigs.

The objective was to determine which tissue components contributed to the reduction in carcass yield of immunologically castrated (IC) barrows when compared to physically castrated (PC) barrows. The carcass yield of an IC barrow is less than the carcass yield of a PC barrow. This has historically been attributed to the presence of testicles, but the testes have only accounted for approximately 0.25% of live weight. This experiment included PC barrows, intact males, IC barrows, IC barrows fed ractopamine hydrochloride, and gilts. When the pigs reached 15 wk old, they were weighed, assigned to treatments (intact male or IC barrow), and penned in groups of 4 pigs per pen. Pigs designated for immunological castration were given injections at approximately 16 wk old and approximately 20 wk old. Pigs were eligible for harvest 33 d after the second injection when the average weight of the pen reached 130 kg. Immunologically castrated barrows lost on average 0.7% units more live weight during transport and lairage than PC barrows, intact males, or gilts. Physically castrated barrows had a 1.43% unit advantage over IC barrows in carcass yield. The differences in yield can be attributed to differences in testicles, reproductive tract, intestinal mass, gut fill, and some visceral organs. Testicle weight accounted for a 0.28% unit reduction in carcass yield of IC barrows when compared to PC barrows. Additional reproductive tract weights accounted for differences of 0.10% units. Intestinal mass (empty large intestine, small intestine, and stomach) was 0.2% units heavier in IC barrows when compared to PC barrows. Livers from IC barrows were 200 g heavier (P < 0.05) and kidneys were 40 g heavier than the same organs in PC barrows. These 2 organs combined for a 0.15% unit difference in carcass yield between IC and PC barrows. Gut fill, testicles, reproductive tract, intestinal mass, and the liver and kidney accounted for 0.97 of 1.43% unit differences in carcass yield between IC and PC barrows. Immunologically castrated barrows had less marbling than PC barrows, but there were no other differences in pork quality parameters. Cutability differences were less than reported in previous experiments, but IC barrows still had a 1.0% unit advantage in lean cutting yield and a 0.7% unit advantage in carcass cutting yield when compared to PC barrows.

The effect of ractopamine hydrochloride (Paylean(®)) on lean carcass yields and pork quality characteristics of heavy pigs fed normal and amino acid fortified diets.

Effects of ractopamine hydrochloride (RAC) on carcass parameters in heavy weight (133.24±8.07kg) finishing pigs (n=278) given amino acid fortified (AA) or 16% crude protein (CP) diets were evaluated. A total of seven experimental diets were formulated; RAC was added at 0, 5 and 20ppm to the 16% CP diets (CP0, CP5 and CP20, respectively) and at 0, 5, 10 and 20ppm to the AA fortified diets (AA0, AA5, AA10 and AA20, respectively). Carcass, tenderloin, and ham weights were heavier (P<0.05) for RAC AA diets vs. AA0. Loin weight was heavier (P<0.05) for AA20 vs. AA0 and CP20 vs. CP0. No differences (P>0.05) were observed for color or firmness scores. Carcass muscle score, ham weight and protein% were greater (P<0.05) for RAC diets. Moisture was greater (P<0.05) and fat was lower (P<0.05) for AA5 and AA20 vs. AA0 and CP5 and CP20 vs. CP0. Feeding RAC to late finishing swine increases carcass yields and protein% with lower fat% for pigs weighing up to 136kg.

Effects of ractopamine supplementation and postmortem aging on longissimus muscle palatability of beef steers differing in biological type.

Effects of ractopamine hydrochloride (RAC) supplementation and postmortem aging on palatability of beef from steers differing in biological type were evaluated using LM samples from British, Continental crossbred, and Brahman crossbred calf-fed steers (n = 98/type). Equal numbers of steers within each type were assigned to treatments of 0 or 200 mg.steer(-1).d(-1) of RAC fed during the final 28 d of the finishing period. Warner-Bratzler shear force (WBSF) was measured at 3, 7, 14, and 21 d postmortem, and trained sensory panel (TP) evaluation was conducted using LM samples aged for 14 d postmortem. A RAC x type interaction (P = 0.006) was detected for WBSF. Within each type, steers fed RAC produced steaks with greater (P < 0.05) WBSF values than steaks from control steers; however, the magnitude of the effect of RAC on WBSF was more pronounced among Brahman cross-breds (5.53 vs. 4.96 +/- 0.10 kg) than among Continental crossbred (4.16 vs. 3.96 +/- 0.10 kg) and British steers (4.10 vs. 3.75 +/- 0.10 kg). The effect of RAC on WBSF, though diminished slightly by aging (mean WBSF difference: 3 d = 0.49 kg; 21 d = 0.24 kg), was not completely mitigated by 21 d of postmortem storage (P(RAC x AGE) = 0.16). Steers fed RAC produced steaks that received lower (P < 0.05) TP ratings for tenderness (8.09 vs. 8.95 +/- 0.18) and juiciness (7.41 vs. 8.07 +/- 0.16 kg), along with slightly lower (P = 0.06) ratings for beef flavor (6.67 vs. 6.93 +/- 0.10 kg), compared with steaks from unsupplemented steers, regardless of biological type. Among the 3 biological types, Brahman crossbred cattle produced steaks with the greatest (P < 0.05) WBSF values at each aging period; WBSF values for steaks from British and Continental type steers did not differ (P > 0.05) at any aging time. Sensory panel ratings of tenderness, juiciness, and beef flavor were greatest (P < 0.05) for steaks from British steers, and least (P < 0.05) for steaks produced by Brahman-type steers. Results from this study suggest that RAC supplementation slightly decreases LM tenderness (WBSF and TP) of British, Continental crossbred, and Brahman cross-bred steers, and that the effect of RAC on WBSF may be more pronounced in steaks from Brahman crossbred cattle than among stenks from Continental type or British steers.

Effects of ractopamine supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type.

Effects of ractopamine hydrochloride (RAC) supplementation on growth performance and carcass characteristics of feedlot steers differing in biological type were investigated using British, Continental crossbred, and Brahman crossbred calf-fed steers (n = 420). Steers of each type were weighed at reimplantation [British, mean BW = 375 kg (SD = 38 kg); Continental crossbred, mean BW = 379 kg (SD = 42 kg); Brahman crossbred, mean BW = 340 (SD = 32 kg)] and sorted into 7 BW blocks, each block consisting of 2 pens (10 steers per pen) per type. Pens within a block x type subclass were randomly assigned to RAC treatments (0 or 200 mg x steer(-1) x d(-1) fed during the final 28 d of the finishing period). The type x RAC interaction did not affect (P > 0.05) any of the traits evaluated in this study. Feeding RAC improved (P = 0.001) ADG (1.50 vs. 1.73 +/- 0.09 kg) and G:F (0.145 vs. 0.170 +/- 0.005), but did not affect (P = 0.48) DMI of steers. Dressing percentage, adjusted fat thickness, KPH percentage, and yield grade were not affected by RAC supplementation. Carcasses of steers fed RAC had heavier (P = 0.01) HCW (359 vs. 365 +/- 4.9 kg), larger (P = 0.046) LM areas (81.7 vs. 84.0 +/- 1.1 cm(2)), and tended (P = 0.07) to have lower mean marbling scores (487 vs. 477 +/- 5.2; Slight = 400, Small = 500) than did carcasses of control steers. Among the 3 biological types, Brahman crossbred steers had the lowest DMI and produced the lightest-weight carcasses that had the lowest mean marbling score (P < 0.05). Compared with Continental crossbred and Brahman crossbred steers, British steers produced carcasses with the greatest (P = 0.001) mean marbling scores. Continental crossbred steers had the heaviest BW and greatest dressing percentages and produced the heaviest carcasses with the largest LM areas (P < 0.05) compared with British and Brahman crossbred steers. In the present study, 28 d of supplementation with RAC at a dosage rate of 200 mg x steer(-1) x d(-1) elicited consistent responses in growth performance and carcass traits among 3 diverse biological cattle types.