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1.
J Lipid Res ; 44(9): 1643-51, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12810826

RESUMO

5alpha-Cyprinol sulfate was isolated from bile of the Asiatic carp, Cyprinus carpio. 5alpha-Cyprinol sulfate was surface active and formed micelles; its critical micellization concentration (CMC) in 0.15 M Na+ using the maximum bubble pressure device was 1.5 mM; by dye solubilization, its CMC was approximately 4 mM. At concentrations >1 mM, 5alpha-cyprinol sulfate solubilized monooleylglycerol efficiently (2.1 molecules per mol micellar bile salt). When infused intravenously into the anesthetized rat, 5alpha-cyprinol sulfate was hemolytic, cholestatic, and toxic. In the isolated rat liver, it underwent little biotransformation and was poorly transported (Tmax congruent with 0.5 micromol/min/kg) as compared with taurocholate. 5alpha-Cyprinol, its bile alcohol moiety, was oxidized to its corresponding C27 bile acid and to allocholic acid (the latter was then conjugated with taurine); these metabolites were efficiently transported. 5alpha-Cyprinol sulfate inhibited taurocholate uptake in COS-7 cells transfected with rat asbt, the apical bile salt transporter of the ileal enterocyte. 5alpha-Cyprinol had limited aqueous solubility (0.3 mM) and was poorly absorbed from the perfused rat jejunum or ileum. Sampling of carp intestinal content indicated that 5alpha-cyprinol sulfate was present at micellar concentrations, and that it did not undergo hydrolysis during intestinal transit. These studies indicate that 5alpha-cyprinol sulfate is an excellent digestive detergent and suggest that a micellar phase is present during digestion in cyprinid fish.


Assuntos
Ácidos e Sais Biliares/química , Ácidos e Sais Biliares/metabolismo , Colestanóis/química , Colestanóis/metabolismo , Animais , Bile/química , Ácidos e Sais Biliares/isolamento & purificação , Ácidos e Sais Biliares/toxicidade , Transporte Biológico , Biotransformação , Carpas/metabolismo , Linhagem Celular , Colestanóis/isolamento & purificação , Colestanóis/toxicidade , Técnicas In Vitro , Mucosa Intestinal/metabolismo , Fígado/metabolismo , Estrutura Molecular , Perfusão , Ratos , Espectrometria de Massas por Ionização por Electrospray , Tensão Superficial
2.
J Lipid Res ; 43(5): 685-90, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-11971938

RESUMO

The Shoebill stork, an enigma phylogenetically, was found to contain as its dominant biliary bile acid 16alpha-hydroxychenodeoxycholic acid, a heretofore undescribed bile acid. The bile acid occurred as its taurine N-acyl amidate; structure was established by nuclear magnetic resonance (NMR) and mass spectrometry (MS). A search for this novel bile acid in other Ciconiiformes showed that it constituted >92% of biliary bile acids in five of nine herons in the Ardidae, but was absent in all other families (Ciconiidae, Threskiornithidae, Scopidae, Phoenicopteridae). The presence of this biochemical trait in the Shoebill stork and certain herons suggests that these birds are closely related.


Assuntos
Ácidos e Sais Biliares/análise , Aves/fisiologia , Ácido Quenodesoxicólico/análogos & derivados , Ácido Quenodesoxicólico/análise , Ácido Quenodesoxicólico/química , Filogenia , Animais , Ácidos e Sais Biliares/química , Aves/classificação , Ácido Quenodesoxicólico/isolamento & purificação , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Especificidade da Espécie
3.
Surg Endosc ; 15(2): 209-12, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11285970

RESUMO

BACKGROUND: We set out to determine whether intravenously administered cholylglycylaminofluorescein (CGF), a fluorescent bile acid, would enhance the visualization of the biliary tract and bile leaks in rabbits undergoing laparoscopic cholecystectomy (LC). METHODS: CGF was infused at doses of 1, 5, and 10 mg/kg b.w. Biliary recovery was determined spectrophotometrically (six rabbits). For LC (seven rabbits), a blue (fluorescein) filter was attached to the light source, and a fluorescein-emission filter was attached to the charge coupled device (CCD) camera. The biliary tract and bile leak (made by incising the gallbladder) was observed under standard and fluorescent illumination. RESULTS: Apple-green fluorescence appeared in 2 min and persisted for 30-60 min, enhancing visualization of bile duct anatomy as well as the bile leak. Biliary recovery of CGF at 90 min was high (86-96% of the infused dose). CONCLUSION: In rabbits, CGF is secreted quantitatively in bile, induces biliary fluorescence, and enhances visualization of the bile ducts and bile leaks when viewed with appropriate filters.


Assuntos
Doenças Biliares/diagnóstico , Colecistectomia Laparoscópica/métodos , Colecistite/diagnóstico , Fluoresceínas , Aumento da Imagem/métodos , Monitorização Intraoperatória/métodos , Animais , Doenças Biliares/cirurgia , Colecistite/cirurgia , Meios de Contraste , Modelos Animais de Doenças , Injeções Intravenosas , Coelhos , Sensibilidade e Especificidade
4.
Gastroenterology ; 117(4): 962-71, 1999 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10500080

RESUMO

BACKGROUND & AIMS: Dihydroxy bile acids induce a bicarbonate-rich hypercholeresis when secreted into canalicular bile in unconjugated form; the mechanism is cholehepatic shunting. The aim of this study was to identify a xenobiotic that induces hypercholeresis by a similar mechanism. METHODS: Five organic acids (sulindac, ibuprofen, ketoprofen, diclofenac, and norfloxacin) were infused into rats with biliary fistulas. Biliary recovery, bile flow, and biliary bicarbonate were analyzed. Sulindac transport was further characterized using Tr(-) rats (deficient in mrp2, a canalicular transporter for organic anions), the isolated perfused rat liver, and hepatocyte membrane fractions. RESULTS: In biliary fistula rats, sulindac was recovered in bile in unconjugated form and induced hypercholeresis of canalicular origin. Other compounds underwent glucuronidation and were not hypercholeretic. In the isolated liver, sulindac had delayed biliary recovery and induced prolonged choleresis, consistent with a cholehepatic circulation. Sulindac was secreted normally in Tr(-) rats, indicating that its canalicular transport did not require mrp2. In the perfused liver, sulindac inhibited cholyltaurine uptake, and when coinfused with cholyltaurine, induced acute cholestasis. With both basolateral and canalicular membrane fractions, sulindac inhibited cholyltaurine transport competitively. CONCLUSIONS: Sulindac is secreted into bile in unconjugated form by a canalicular bile acid transporter and is absorbed by cholangiocytes, inducing hypercholeresis. At high flux rates, sulindac competitively inhibits canalicular bile salt transport; such inhibition may contribute to the propensity of sulindac to induce cholestasis in patients.


Assuntos
Anti-Inflamatórios não Esteroides/metabolismo , Canalículos Biliares/metabolismo , Ductos Biliares/irrigação sanguínea , Bile/metabolismo , Circulação Hepática , Sulindaco/metabolismo , Animais , Anti-Inflamatórios não Esteroides/sangue , Bicarbonatos/metabolismo , Bile/fisiologia , Fístula Biliar/metabolismo , Transporte Biológico/efeitos dos fármacos , Biotransformação , Técnicas In Vitro , Cinética , Masculino , Perfusão , Ratos , Ratos Sprague-Dawley , Sulindaco/antagonistas & inibidores , Sulindaco/sangue , Sulindaco/farmacocinética , Ácido Taurocólico/metabolismo
5.
J Lipid Res ; 39(11): 2119-24, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9799797

RESUMO

jj biliary bile acid composition of the adult and neonatal domestic rabbit, as well as that of the adult brush rabbit, was characterized. In adult domestic rabbits, the dominant bile acid present was deoxycholic acid (88% of total bile acids), a secondary bile acid formed by the bacterial 7-dehydroxylation of cholic acid. Although most of the bile acids present were conjugated with glycine, two exceptions were observed. About 3% of deoxycholic acid was conjugated, in N-acyl linkage, with glycyl-taurine. Chenodeoxycholic acid, which composed <1% of wile acids, was conjugated solely with taurine. The bile of neonatal rabbits contained a greater percentage of primary bile acids, and bile acids were conjugated to a much greater extent with taurine. The adult brush rabbit had a bile acid composition similar to that of the domestic rabbit, but about one-third of all bile acids were conjugated with taurine. In addition, lithocholic acid was present as its sulfated amidate, whereas in the domestic rabbit, lithocholic acid was conjugated solely with glycine. The biliary bile acid composition of rabbits appears to be unique both in terms of the predominant steroid moiety, as well as in the modes of conjugation.


Assuntos
Ácidos e Sais Biliares/química , Sistema Biliar/metabolismo , Ácido Desoxicólico/análogos & derivados , Coelhos/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Glicosilação , Masculino , Espectrometria de Massas , Taurina/análise
6.
Hepatology ; 28(2): 510-20, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9696018

RESUMO

Hepatocyte transport of six fluorescent bile acids containing nitrobenzoxadiazolyl (NBD) or a fluorescein derivative on the side chain was compared with that of natural bile acids using the single-pass perfused rat liver. Compounds were infused at 40 nmol/g liver min for 15 minutes; hepatic uptake and biliary recovery were measured; fractional extraction, intrinsic basolateral clearance, and sequestration (nonrecovery after 45 minutes of additional perfusion) were calculated. Fluorescent bile acids were efficiently extracted during the first 3 minutes (70%-97%), but net extraction decreased with time mostly because of regurgitation into the perfusate. For cholylglycine and ursodeoxycholylglycine (UDC-glycine), extraction was 94% to 99%, and regurgitation did not occur. Intrinsic hepatic clearance of fluorescent bile acids (2-7 mL/g liver x min) was lower than that of cholylglycine (9.0 +/- 0.6; mean +/- SD) and UDC-glycine (21.4 +/- 0.4). Sequestration at 60 minutes was 8% to 26% for fluorescent bile acids with a cholyl moiety (cholylglycylaminofluorescein [CGamF], cholyllysylfluorescein [C-L-F], cholyl-[N epsilon-NBD]-lysine [C-L-NBD], and cholylaminofluorescein [CamF]), 32% for ursodeoxycholylaminofluorescein (UDCamF), and 88% for ursodeoxycholyl-(N epsilon-NBD)lysine (UDC-L-NBD). Cholylglycine and UDC-glycine had <3% retention. Biliary secretion of sequestered UDCamF, but not of UDC-L-NBD, was induced by adding dibutyryl cyclic adenosine monophosphate (DBcAMP) to the perfusate, possibly by translocation to the canaliculus of pericanalicular vesicles containing fluorescent bile acids. Biliary secretion of UDC-L-NBD, but not of UDCamF, was induced by adding cholyltaurine or UDC-taurine, possibly by inhibition of binding to intracellular constituents or of transport into organelles. It is concluded that fluorescent bile acids are efficiently transported across the basolateral membrane, but in contrast to natural conjugated bile acids, are sequestered in the hepatocyte (UDC derivatives > cholyl derivatives). Two modes of hepatic sequestration of fluorescent bile acids were identified. Fluorescent bile acids may be useful to characterize sequestration processes during bile acid transport through the hepatocyte.


Assuntos
Ácidos e Sais Biliares/metabolismo , Fígado/metabolismo , Animais , Ácidos e Sais Biliares/farmacologia , Ácidos e Sais Biliares/fisiologia , Transporte Biológico/efeitos dos fármacos , Transporte Biológico/fisiologia , Bucladesina/farmacologia , Fluorescência , Técnicas In Vitro , Cinética , Fígado/efeitos dos fármacos , Masculino , Perfusão , Ratos , Ratos Sprague-Dawley , Fatores de Tempo
7.
Am J Physiol ; 274(2): G370-5, 1998 02.
Artigo em Inglês | MEDLINE | ID: mdl-9486191

RESUMO

It has been proposed that the hepatocellular Na(+)-dependent bile salt uptake system exhibits a broad substrate specificity in intact hepatocytes. In contrast, recent expression studies in mammalian cell lines have suggested that the cloned rat liver Na(+)-taurocholate cotransporting polypeptide (Ntcp) may transport only taurocholate. To characterize its substrate specificity Ntcp was stably transfected into Chinese hamster ovary (CHO) cells. These cells exhibited saturable Na(+)-dependent uptake of [3H]taurocholate [Michaelis constant (K(m)) of approximately 34 microM] that was strongly inhibited by all major bile salts, estrone 3-sulfate, bumetanide, and cyclosporin A. Ntcp cRNA-injected Xenopus laevis oocytes and the transfected CHO cells exhibited saturable Na(+)-dependent uptake of [3H]taurochenodeoxycholate (Km of approximately 5 microM), [3H]tauroursodeoxycholate (Km of approximately 14 microM), and [14C]glycocholate (Km of approximately 27 microM). After induction of gene expression by sodium butyrate, Na(+)-dependent transport of [3H]estrone 3-sulfate (Km of approximately 27 microM) could also be detected in the transfected CHO cells. However, there was no detectable Na(+)-dependent uptake of [3H]bumetanide or [3H]cyclosporin A. These results show that the cloned Ntcp can mediate Na(+)-dependent uptake of all physiological bile salts as well as of the steroid conjugate estrone 3-sulfate. Hence, Ntcp is a multispecific transporter with preference for bile salts and other anionic steroidal compounds.


Assuntos
Proteínas de Transporte/metabolismo , Fígado/metabolismo , Oócitos/metabolismo , Transportadores de Ânions Orgânicos Dependentes de Sódio , Simportadores , Animais , Células CHO , Cricetinae , Estrona/análogos & derivados , Estrona/metabolismo , Ratos , Especificidade por Substrato , Ácido Taurocólico/farmacocinética , Xenopus laevis
8.
Hepatology ; 26(5): 1263-71, 1997 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9362371

RESUMO

Studies were performed to characterize hepatic and intestinal transport, as well as biotransformation during transport, of a spectrum of fluorescent bile acids containing a fluorophore attached to the side chain. The following two classes of compounds were studied: 1) aminofluorescein (amF) coupled directly to the carboxylic group of a bile acid which was cholic, ursodeoxycholic, or cholylglycine; and 2) nitrobenzoxadiazolyl (NBD) coupled to the epsilon-amino group of a lysine conjugated bile acid, which was cholic or ursodeoxycholic. Fluorescein, a cholephilic organic anion, was studied as a control. Fluorescent bile acids were synthesized and their structures confirmed by nuclear magnetic resonance and mass spectrometry. Using the biliary fistula rat, hepatic transport, biotransformation, and choleretic activity were defined; intestinal absorption was assessed by jejunal or ileal perfusion studies. All fluorescent bile acids had hepatic transport maxima about one-sixth that reported for cholyltaurine, but derivatives of cholylglycine were transported best. Bile acids underwent little (<5%) biotransformation during hepatocyte transport. Only the amF conjugate of cholylglycine had normal choleretic activity; other compounds were hypocholeretic or cholestatic. In contrast, fluorescein was well transported, was partly glucuronidated, and had normal choleretic activity. NBD-tagged, but not amF-tagged, bile acids were actively transported by the intestine (ileum > jejunum), and no fluorescent bile acid had passive intestinal permeability; NBD-tagged bile acids were biotransformed during intestinal transport (jejunum > ileum). We conclude that the structure of the fluorophore as well as that of the bile acid influences transport by the hepatocyte and enterocyte. These fluorescent bile acids differ from fluorescein in being impermeable to cell membranes and undergoing little biotransformation during hepatocyte transport. Of these fluorescent bile acids, cholylglycylamF has hepatocyte transport and choleretic properties most closely resembling those of a natural bile acid.


Assuntos
Ácidos e Sais Biliares/química , Ácidos e Sais Biliares/farmacocinética , Fluorescência , Mucosa Intestinal/metabolismo , Fígado/metabolismo , Animais , Bile/metabolismo , Ácidos e Sais Biliares/farmacologia , Transporte Biológico/fisiologia , Biotransformação/fisiologia , Colagogos e Coleréticos/farmacologia , Absorção Intestinal/fisiologia , Masculino , Ratos , Ratos Sprague-Dawley
9.
Exp Parasitol ; 87(2): 133-41, 1997 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9326888

RESUMO

Giardia lamblia trophozoites colonize the human small intestine, where they are exposed to high concentrations of conjugated bile acids. Previous work has shown that bile acids enhance trophozoite survival, multiplication, and differentiation into the cyst stage. Therefore, experiments were performed to test whether carrier-mediated uptake of conjugated bile acids is present in this primitive parasite. Uptake of both cholyltaurine (C-tau) and cholylglycine (C-gly) was increased manyfold after culturing trophozoites in medium lacking bile acids. Absence of uptake at 4 degrees C and inhibition by other conjugated bile acids provided additional evidence for carrier-mediated uptake. Uptake of C-tau was greater than that of C-gly under all experimental conditions and appeared to be mediated by a different carrier. The major evidence for different carriers is that C-tau uptake was Na(+)-dependent, while C-gly uptake was not. In addition, C-tau uptake was more strongly inhibited by DTNB and several organic anions than C-gly uptake. Radiolabeled C-tau and C-gly were each released rapidly from trophozoites at 37 degrees C but not at 4 degrees C, suggesting that release of conjugated bile acids was also carrier-mediated. These findings are consistent with the notion that multiple transporters for conjugated bile acids are present in a lower eukaryote. We speculate that intracellular bile acids may facilitate lipid trafficking and membrane biosynthesis.


Assuntos
Ácidos e Sais Biliares/metabolismo , Giardia lamblia/metabolismo , Animais , Transporte Biológico , Bovinos , Ácido Ditionitrobenzoico/farmacologia , Ditiotreitol/farmacologia , Giardia lamblia/efeitos dos fármacos , Ácido Glicocólico/metabolismo , Humanos , Reagentes de Sulfidrila/farmacologia , Ácido Taurocólico/metabolismo
10.
Hepatology ; 26(2): 374-85, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9252148

RESUMO

To assess the effect of side chain length on the metabolism and physiological effects of homologues of chenodeoxycholic acid (CDCA), dinorCDCA, the C22 homologue, was synthesized and its hepatic biotransformation, transport kinetics, and choleretic properties were defined in rat and hamster biliary fistula and in isolated perfused rat liver. Results were compared with those of norCDCA, the C23 homologue, and of CDCA, the natural C24 homologue. In the rat, dinorCDCA was secreted mostly in unconjugated form (the majority as dinor-alpha-muricholic acid); the remainder was glucuronidated. In the hamster, glucuronidation was greater, and the unconjugated fraction contained equal parts of dinorCDCA and 5beta-hydroxy-dinorCDCA. NorCDCA was glucuronidated extensively (70%, rat; 40%, hamster). CDCA, in contrast, was efficiently amidated with taurine or glycine. In the perfused liver, the initial uptake rate of all three homologues was identical; later, regurgitation and/or cholehepatic shunting of dinorCDCA and norCDCA, but not of CDCA, occurred. In rats and hamsters with biliary fistulas, dinorCDCA and norCDCA, but not CDCA, induced a bicarbonate-rich hypercholeresis of canalicular origin. Hypercholeresis was not induced by the taurine conjugate of dinorCDCA. Hepatobiliary retention of both dinorCDCA and norCDCA occurred, consistent with efficient ductular absorption (calculated to be 94%) and cholehepatic cycling of the unmetabolized bile acids. It is concluded that dinorCDCA, as norCDCA, is inefficiently amidated, is metabolized as a xenobiotic, and induces hypercholeresis. DinorCDCA is the first dihydroxy bile acid to be identified that is secreted largely in unconjugated form in bile.


Assuntos
Ácido Quenodesoxicólico/farmacocinética , Colagogos e Coleréticos/farmacologia , Fígado/metabolismo , Animais , Fístula Biliar/metabolismo , Transporte Biológico , Biotransformação , Ácido Quenodesoxicólico/análogos & derivados , Ácido Quenodesoxicólico/farmacologia , Cricetinae , Ratos , Solubilidade , Relação Estrutura-Atividade
11.
Am J Physiol ; 272(6 Pt 1): G1416-24, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9227477

RESUMO

When secreted into bile, unconjugated dihydroxy bile acids are absorbed passively by cholangiocytes according to the cholehepatic circulation hypothesis. A fraction of these are likely to be conjugated during transcellular transport. Experiments were performed using fluorescent conjugated bile acids to test whether carrier-mediated transport of conjugated bile acids is present in the basolateral domains of polarized cholangiocytes of intrahepatic bile ductules isolated from rat liver. The time course of the cellular localization of cholyl-NBDAB-Gly and chenodeoxycholyl-NBDAB-Gly, which are anionic fluorescent derivatives of the corresponding glycine-conjugated bile acids, was characterized using an image-analysis system. With 0.3-3 microM solutions, fluorescence was present at 1 and 3 min in the basolateral area of cholangiocytes. Staining in the apical region occurred later, with a peak after 15 min of incubation. The basolateral uptake of the two fluorescent bile acids was temperature dependent and Na+ independent, and was not influenced by the addition of amiloride, by lowering of the medium pH to 6.0, or by preincubation with valinomycin. Uptake was partially inhibited by the absence of Cl- or HCO3- in the perfusate, by preincubation with 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), and by the presence of different organic anions or unconjugated and conjugated bile acids in the medium. When cells were preloaded with an ethyl ester of chenodeoxycholyl-NBDAB-Gly, which is hydrolyzed by intracellular esterases, the decrease of cell fluorescence was partly inhibited by H2DIDS, whereas it was stimulated by the presence of 20 microM cholyltaurine in the medium. It is concluded that transport of conjugated bile acid anions across the basolateral membrane of the polarized rat cholangiocyte is carrier mediated. The conjugated bile acid transporter is likely to be an anion exchanger and is likely to be involved in bile secretion whenever conjugated bile acids or other organic anions are transported from the base of the biliary ductular epithelial cells into the plasma of the periductular capillary plexus.


Assuntos
Ácidos e Sais Biliares/metabolismo , Ductos Biliares Intra-Hepáticos/metabolismo , Proteínas de Transporte/metabolismo , Membrana Celular/metabolismo , Ácido Quenodesoxicólico/análogos & derivados , Ácidos Cólicos/metabolismo , Glicina/análogos & derivados , Hidroxiesteroide Desidrogenases , Glicoproteínas de Membrana , Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico/farmacologia , Animais , Ácidos e Sais Biliares/farmacologia , Proteínas de Transporte/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Polaridade Celular , Ácido Quenodesoxicólico/metabolismo , Epitélio/metabolismo , Corantes Fluorescentes , Furosemida/farmacologia , Glicina/metabolismo , Cinética , Fígado , Masculino , Probenecid/farmacologia , Ratos , Ratos Sprague-Dawley , Sulfobromoftaleína/farmacologia
12.
Dig Dis Sci ; 42(6): 1274-82, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9201095

RESUMO

Topical dissolution of cholesterol gallbladder stones using methyl tert-butyl ether (MTBE) is useful in symptomatic patients judged too ill for surgery. Previous studies showed that ethyl propionate (EP), a C5 ester, dissolves cholesterol gallstones rapidly in vitro, but differs from MTBE in being eliminated so rapidly by the liver that blood levels remain undetectable. Our aim was to test EP as a topical dissolution agent for cholesterol gallbladder stones. Five high-risk patients underwent topical dissolution of gallbladder stones by EP. In three patients, the solvent was instilled via a cholecystostomy tube placed previously to treat acute cholecystitis; in two patients, a percutaneous transhepatic catheter was placed in the gallbladder electively. Gallstone dissolution was assessed by chromatography, by gravimetry, and by catheter cholecystography. Total dissolution of gallstones was obtained in four patients after 6-10 hr of lavage; in the fifth patient, partial gallstone dissolution facilitated basketing of the stones. In two patients, cholesterol dissolution was measured and averaged 30 mg/min. Side effects were limited to one episode of transient hypotension and pain at the infusion site; no patient developed somnolence or nausea. Gallstone elimination was associated with relief of symptoms. EP is an acceptable alternative to MTBE for topical dissolution of cholesterol gallbladder stones in high-risk patients. The lower volatility and rapid hepatic extraction of EP suggest that it may be preferable to MTBE in this investigational procedure.


Assuntos
Colelitíase/terapia , Colesterol/análise , Propionatos/uso terapêutico , Solventes/uso terapêutico , Idoso , Colecistostomia , Colelitíase/química , Feminino , Humanos , Instilação de Medicamentos , Masculino , Éteres Metílicos/uso terapêutico , Pessoa de Meia-Idade , Fatores de Tempo
13.
Hepatology ; 25(6): 1457-61, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9185768

RESUMO

In iron overload, non-transferrin-bound iron (NTBI) is found in plasma and is rapidly removed by hepatocytes. Some of this NTBI is excreted into bile. Biliary excretion of NTBI, in the form of an iron-deferiprone chelate, is greatly increased by deferiprone, an iron chelator. The aim of this study was to test whether biliary iron as such or as an iron-deferiprone chelate (both originating from plasma NTBI) is absorbed from the intestine and re-secreted into bile. In healthy biliary fistula (donor) rats, biliary 55Fe originating from plasma NTBI was obtained by injecting Fe citrate (to saturate transferrin) followed by 55Fe. This biliary 55Fe was infused into the duodenum of (recipient) rats whose transferrin was saturated or unsaturated. Similar experiments were performed using iron-overloaded (donor) rats given deferiprone, followed by infusion of the biliary 55Fe-deferiprone chelate into iron-overloaded (recipient) rats. The results show that in healthy (recipient) rats, duodenal infusion of 55Fe from NTBI was followed by increased plasma 55Fe when transferrin was unsaturated, or by biliary excretion of 55Fe when transferrin was saturated, indicating intestinal absorption of 55Fe. In iron-overloaded rats, neither plasma nor bile became radioactive, indicating no intestinal absorption of iron from the deferiprone chelate. We conclude that biliary iron, originating from NTBI, is absorbed from the intestine, and undergoes enterohepatic circulation if transferrin is saturated. In iron-overloaded rats, biliary iron originating from plasma NTBI and present as an iron-deferiprone chelate in bile is not absorbed.


Assuntos
Bile/metabolismo , Absorção Intestinal , Mucosa Intestinal/metabolismo , Ferro/sangue , Ferro/metabolismo , Fígado/metabolismo , Animais , Deferiprona , Compostos Férricos/farmacologia , Injeções Intravenosas , Quelantes de Ferro/farmacologia , Sobrecarga de Ferro/metabolismo , Masculino , Piridonas/farmacologia , Ratos , Ratos Sprague-Dawley , Valores de Referência , Transferrina/metabolismo
14.
Am J Physiol ; 272(3 Pt 1): G498-506, 1997 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9124570

RESUMO

Experiments were performed to define the mechanism of intestinal absorption of dodecyl sulfate (DS), an amphipathic organic anion whose chemical structure resembles that of dodecanoate, a C12 fatty acid anion. With jejunal segments perfused in single-pass fashion in the anesthetized rat, steady-state absorption of DS was concentration dependent, with the apparent permeability constant (P(app)) ranging from 4 to 22 x 10(-5) cm/s. When DS concentration was held constant and net water absorption was induced by decreasing perfusate osmolality, DS absorption increased in direct proportion to water absorption, suggesting absorption by solvent drag via the paracellular route. However, DS absorption continued even when water secretion was induced by a hypertonic perfusate. Consequently, for all experiments, DS absorption could be empirically described as the sum of two terms: 1) absorption in the absence of water absorption (P(app) = 5.6 x 10(-5) cm/s) and 2) absorption induced by water movement [(delta P(app)/delta water absorption) = 0.2 x 10(-5) cm x s(-1) x microl segment(-1) x min(-1)]. In a polarized epithelial monolayer of renal epithelial cells (Madin-Darby canine kidney cells), DS was absorbed predominantly by a paracellular pathway, as the absorption rate increased threefold when paracellular junction pore size was increased by the addition of cytochalasin D. The calculated apparent radius was 2.9 A, indicating that the cross section of the molecule, not its length, determined the rate of absorption. It is concluded that absorption of DS in the intact animal occurs slowly and mostly via the paracellular route, because the fixed negative charge on the molecule retards rapid passive entry into the enterocyte, as occurs with protonated fatty acids. That absorption of DS persisted despite net water secretion suggests a low level of transcellular absorption across the jejunal enterocyte also occurs.


Assuntos
Absorção Intestinal , Dodecilsulfato de Sódio/metabolismo , Animais , Linhagem Celular , Cães , Ácidos Graxos/metabolismo , Cobaias , Técnicas In Vitro , Jejuno/metabolismo , Modelos Biológicos , Permeabilidade , Ratos , Equilíbrio Hidroeletrolítico
15.
Gastroenterology ; 112(1): 214-25, 1997 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8978362

RESUMO

BACKGROUND & AIMS: In sepsis, intrahepatic cholestasis occurs frequently, suggesting impaired hepatocyte transport of bile acids and organic anions. The aim of the study was to define the magnitude, time course, and the site of impaired biliary secretion in a rat sepsis model. METHODS: Maximal transport for two bile acids (cholyltaurine and chenodeoxycholyltaurine) and two organic anions (sulfobromophthalein and sulfolithocholyltaurine) was measured in isolated perfused livers at various times after lipopolysaccharide injection. Basolateral and canalicular liver plasma membrane vesicles were used to characterize the impairment in hepatocyte transport. RESULTS: Maximal hepatocyte transport was reduced for all compounds by 60%-81% compared with controls. Bile acid-independent bile flow was reduced by 51%. Impairment was maximal 12 hours after endotoxin injection and recovered thereafter. In basolateral plasma membrane vesicles, sodium-dependent transport for bile acids was reduced by 36%-47%. Sodium-independent transport of organic anions was reduced by 40%-55%. Adenosine triphosphate-stimulated transport was greatly decreased in canalicular vesicles prepared from endotoxemic animals for all four compounds probably because of a reduced number of transport molecules, based on kinetic studies. CONCLUSIONS: Basolateral and canalicular bile acid and organic anion transport are markedly impaired in endotoxemia. These mechanisms may contribute to the cholestasis of sepsis.


Assuntos
Colestase/etiologia , Lipopolissacarídeos/toxicidade , Fígado/metabolismo , Ácido Tauroquenodesoxicólico/metabolismo , Ácido Taurocólico/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Bile/metabolismo , Transporte Biológico/efeitos dos fármacos , Membrana Celular/metabolismo , Ratos
16.
J Lipid Res ; 37(6): 1189-97, 1996 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8808753

RESUMO

The metabolism of 3 alpha,7 alpha-dihydroxy-25,26-bishomo-5 beta-cholane-26-sulfonate (bishomoCDC-sul), the sulfonate analogue of bishomochenodeoxycholic acid, and its effect on biliary bile acid composition were studied during chronic administration in the hamster. After oral administration of radiolabeled bishomoCDC-sul, more than 80% of the radioactivity was excreted into the feces within 7 days, both as the unchanged sulfonate (38.5%) and two more polar metabolites (50.0% and 11.5%). The half time of the fecal excretion was 1.6 days. In gallbladder bile, the unchanged sulfonate and its major metabolite accounted for 19.1% and 19.8% of total bile acids, respectively. In another experiment, hamsters were fed bishomoCDC-sul with antibiotics to evaluate the site of biotransformation. Even when the number of intestinal microorganisms was greatly reduced, the same three metabolites were found in the feces: bishomoCDC-sul (44.0%) and the two polar metabolites (30.8% and 25.1%). The major metabolite was isolated from feces of the hamsters fed bishomoCDC-sul without antibiotics. Its chemical structure was identified by mass spectrometry and nuclear magnetic resonance spectroscopy as the 15 alpha-hydroxylated derivative, namely sodium 3 alpha,7 alpha,15 alpha-trihydroxy-25,26-bishomo-5 beta-cholane-26-sulfonate. These results indicate that after oral administration, the sulfonate analogue of bishomochenodeoxycholic acid underwent enterohepatic circulation like a natural bile acid and was transformed, in part, into the 15 alpha-hydroxylated derivative and another more polar metabolite in the liver of hamsters. There was no evidence that bishomoCDC-sul was dehydroxylated to a lithocholic acid analogue during enterohepatic cycling.


Assuntos
Ácidos e Sais Biliares/metabolismo , Bile/fisiologia , Ácido Quenodesoxicólico/análogos & derivados , Animais , Bile/efeitos dos fármacos , Biotransformação , Ácido Quenodesoxicólico/farmacocinética , Ácido Quenodesoxicólico/farmacologia , Cricetinae , Hidroxilação , Espectroscopia de Ressonância Magnética , Masculino , Estrutura Molecular , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Fatores de Tempo
17.
Gastroenterology ; 110(4): 1098-106, 1996 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-8612999

RESUMO

BACKGROUND & AIMS: Conjugated bile acid absorption is known to occur in the jejunum in mammals, but the mechanism has not been well defined. The aim of this study was to define the mechanisms by which conjugated bile acids are absorbed from the jejunum. METHODS: The steady-state absorption of eight conjugated bile acids from a perfused jejunal segment was measured in the anesthetized biliary fistula guinea pig. Experiments defined the effect of bile acid structure, tested for competitive inhibition and saturation of transport, and compared the absorption rate of taurine conjugates with that of glycine conjugates at pH 7.6 or 5.0. RESULTS: Dihydroxy conjugates were absorbed twice as rapidly as cholyl conjugates from the perfused jejunum; glycine and taurine conjugates of a given bile acid were absorbed at a similar rate. Absorption of ursodeoxycholate taurine showed saturability and competitive inhibition by other conjugated bile acids. When intraluminal pH was decreased to pH 5.0, the absorption rate of glycine (but not taurine) conjugates increased, indicating passive absorption of the protonated species of glycine-conjugated bile acids. CONCLUSIONS: Uptake of glycine- or taurine-conjugated bile acids by the guinea pig jejunum occurs by at least two mechanisms: carrier-mediated transport (dihydroxy conjugates greater than trihydroxy conjugates) and passive absorption in protonated (uncharged) form of glycine conjugates.


Assuntos
Ácidos e Sais Biliares/metabolismo , Proteínas de Transporte/metabolismo , Absorção Intestinal , Jejuno/metabolismo , Animais , Ácidos e Sais Biliares/química , Glicina/metabolismo , Cobaias , Concentração de Íons de Hidrogênio , Prótons , Taurina/metabolismo
18.
J Lipid Res ; 37(2): 431-6, 1996 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9026540

RESUMO

Bile acids labeled with 3H on the sterol nucleus lose a substantial fraction of label during enterohepatic cycling and conversion to secondary bile acids. We tested the isotopic stability of a side-chain 3H label, [22,23-3H]cholic acid in humans. The 3H-labeled compound was administered simultaneously with [24-14C]cholic acid to four healthy volunteers. Duodenal bile was collected daily for 5 days after isotope administration to determine the ratio of 3H/14C in bile acids. Urine was collected to determine loss of radioactivity by this route. Cholic acid and deoxycholic acid were isolated from biliary bile acids by thin-layer chromatography after deconjugation with cholylglycine hydrolase. The ratio of 3H/14C in cholic acid and deoxycholic acid remained constant and identical to that of the administered mixture in all subjects, indicating stability of the 3H label during enterohepatic cycling. Cumulative loss of 3H in urine averaged only 1.2% of administered dose and was identical to loss of 14C (average 1.3%) indicating little if any transfer of 3H from bile acid to body water. Deconjugation of biliary bile acids by alkaline hydrolysis resulted in 15-20% loss of 3H label, consistent with known base-catalyzed exchange of alpha-carbon protons on carboxylic acids. We conclude that [22,23-3H]cholic acid is a biologically stable, and therefore reliable, isotopic tracer of cholic acid in humans during enterohepatic cycling including conversion to deoxycholic acid, provided deconjugation is performed enzymatically. Because the 22,23-3H label can be inserted into most C24 bile acids, it appears the best way to tag 3H-labeled bile acids for metabolic studies.


Assuntos
Ácidos Cólicos/metabolismo , Ácido Desoxicólico/metabolismo , Trítio , Adulto , Idoso , Bile/metabolismo , Radioisótopos de Carbono/urina , Ácido Cólico , Cromatografia em Camada Fina , Estabilidade de Medicamentos , Humanos , Marcação por Isótopo , Cinética , Masculino , Pessoa de Meia-Idade , Trítio/urina
19.
J Lipid Res ; 37(1): 98-112, 1996 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8820106

RESUMO

The hepatic biotransformation in the rat and hamster of 23(R)-hydroxychenodeoxycholic acid (23(R)OH-CDCA), the alpha-hydroxy derivative of CDCA, was defined; some physiological and physicochemical properties were also assessed. 23(R)OH-CDCA was isolated from duck bile; [24-14C]23(R)OH-CDCA was synthesized. The compound was administered intravenously to anesthetized biliary fistula rats at doses of 1, 3, or 5 mu mol/kg-min and to hamsters at 3 mu mol/min-kg. Biliary bile acids and radioactivity were analyzed by thin-layer chromatography (TLC), high pressure liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS). Recovery of radioactivity in bile was incomplete (50-70% of infused dose); some was also recovered as breath 14CO2. Radioactivity in bile was present as unchanged compound (25-50%, dose-dependent) and its conjugates (with taurine, with glycine, or with glucuronate). Nor-CDCA (C23) was present in bile (in both unconjugated and conjugated form), indicating that 23(R)OH-CDCA had undergone oxidative decarboxylation (alpha-oxidation) with loss of the C-24 carboxyl group. The alpha-oxidation was 20 +/- 5% (mean +/- SD) of administered dose in the rat and was not dose-dependent; in hamsters, alpha-oxidation was 35 +/- 8%. In rats, the S isomer of 23OH-CDCA also underwent alpha-oxidation (10 +/- 2%). Nor-CDCA also underwent 6beta-hydroxylation to form nor-alpha-muricholic acid, as well as reduction of its C-23 carboxyl group to form the C23 alcohol. The taurine conjugate of 23(R)OH-CDCA [23(R)OH-CDC-tau] was prepared synthetically and characterized by 1H-NMR. By surface tension measurements, it had a critical micellization concentration (CMC) of 3.5 mM (in 0.15 M Na+), as compared to 1.8 mM for CDC-taurine. Aqueous solubility of 23(R)OH-CDCA increased markedly above pH 5, compared to pH 7 for CDCA. When incubated with cholylglycine hydrolase, 23(R)OH-CDC-tau was deconjugated at a rate one-fourth that of CDC-tau. It is concluded that the presence of a 23(R)-hydroxyl group in a 3alpha, 7alpha-dihydroxy bile acid alters its metabolism in the rodent hepatocyte, as evidenced by inefficient conjugation with taurine or glycine, alpha-oxidation to nor (C23) bile acid, and reduction of the nor bile acid to the primary alcohol. The taurine conjugate of 23(R)OH-CDCA, a major biliary bile acid of marine mammals and wading birds, is a biological detergent with properties superior to those of the taurine conjugate of CDCA. Natural C23 nor-bile acids may be formed by alpha-oxidation of alpha-hydroxy C24 bile acids.


Assuntos
Ácido Quenodesoxicólico/análogos & derivados , Fígado/metabolismo , Animais , Bile/química , Bile/metabolismo , Transporte Biológico , Biotransformação , Testes Respiratórios , Ácido Quenodesoxicólico/química , Ácido Quenodesoxicólico/metabolismo , Cricetinae , Patos , Masculino , Mesocricetus , Oxirredução , Ratos , Ratos Sprague-Dawley , Estereoisomerismo
20.
J Lipid Res ; 36(12): 2639-50, 1995 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8847490

RESUMO

Some commercial preparations of common natural conjugated bile salts contain impurities (e.g., amines, lipids, and calcium) that are likely to affect their physicochemical properties. A method was developed for purifying commercial preparations of sodium salts of glycine- and taurine-conjugated bile acids. The method consists of passage of a dilute aqueous solution of the sodium bile salt through three columns in sequence: graphitized carbon, a hydrophobic bonded octadecylsilane (C18) cartridge, and a calcium-chelating resin. The final solution was extracted with chloroform, and the purified bile salt was then isolated by freeze-drying, with a yield of 65-75%. Each bile salt purified by this method was compared with the corresponding bile salt purified by conventional adsorption chromatography on a silicic acid column, using a mixture of methanol and chloroform as eluant. Purity was assessed by visible spectra, by surface tension measurements (using the maximum bubble-pressure method and a Wilhelmy wire method), by chloroform extractability of impurities in the conjugated bile acid, by liposome solubilization, and by chemical analysis of the calcium content. Both purification methods removed colored and surface-active impurities, but the new method was always as or more effective than silicic acid column chromatography. Calcium ion, present in commercial bile salts in concentrations up to 16 mmol/mol bile salt, was removed completely by the three-column method, but not by silicic acid chromatography. The new method is thus a simple, rapid, and efficient procedure for purification of the sodium salts of glycine- and taurine-conjugated bile acids for physicochemical measurements, in which elimination of surface-active impurities and polyvalent cations is desired.


Assuntos
Ácidos e Sais Biliares/química , Cálcio/isolamento & purificação , Cromatografia Líquida/métodos , Tensoativos/isolamento & purificação , Clorofórmio , Contaminação de Medicamentos , Lipídeos/química , Ácido Silícico , Tensão Superficial
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