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1.
Methods Mol Biol ; 1522: 93-106, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27837533

RESUMO

Click chemistry represents a new bioconjugation strategy that can be used to conveniently attach various ligands to the surface of preformed liposomes. This efficient and chemoselective reaction involves a Cu(I)-catalyzed azide-alkyne cycloaddition which can be performed under mild experimental conditions in aqueous media. Here we describe the application of a model click reaction to the conjugation, in a single step, of unprotected α-1-thiomannosyl ligands, functionalized with an azide group, to liposomes containing a terminal alkyne-functionalized lipid anchor. Excellent coupling yields have been obtained in the presence of bathophenanthroline disulfonate, a water soluble copper-ion chelator, acting as a catalyst. No vesicle leakage is triggered by this conjugation reaction and the coupled mannose ligands are exposed at the surface of the liposomes. The major limitation of Cu(I)-catalyzed click reactions is that this conjugation is restricted to liposomes made of saturated (phospho)lipids. To circumvent that constraint, an example of alternative copper-free azide-alkyne click reaction has been developed. Molecular tools and results are presented here.


Assuntos
Química Click/métodos , Lipossomos/química , Cobre/química , Ligantes , Lipídeos/síntese química , Lipídeos/química
2.
J Med Chem ; 58(8): 3582-92, 2015 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-25803425

RESUMO

The blood fluke Schistosoma mansoni is the causative agent of the intestinal form of schistosomiasis (or bilharzia). Emergence of Schistosoma mansoni with reduced sensitivity to praziquantel, the drug currently used to treat this neglected disease, has underlined the need for development of new strategies to control schistosomiasis. Our ability to screen drug libraries for antischistosomal compounds has been hampered by the lack of validated S. mansoni targets. In the present work, we describe a virtual screening approach to identify inhibitors of S. mansoni NAD(+) catabolizing enzyme (SmNACE), a receptor enzyme suspected to be involved in immune evasion by the parasite at the adult stage. Docking of commercial libraries into a homology model of the enzyme has led to the discovery of two in vitro micromolar inhibitors. Further structure-activity relationship studies have allowed a 3-log gain in potency, accompanied by a largely enhanced selectivity for the parasitic enzyme over the human homologue CD38.


Assuntos
Antiparasitários/química , Inibidores Enzimáticos/química , Proteínas de Helminto/antagonistas & inibidores , NAD/metabolismo , Schistosoma mansoni/enzimologia , Esquistossomose mansoni/tratamento farmacológico , Esquistossomose mansoni/parasitologia , ADP-Ribosil Ciclase 1/metabolismo , Animais , Antiparasitários/farmacologia , Inibidores Enzimáticos/farmacologia , Proteínas de Helminto/metabolismo , Humanos , Simulação de Acoplamento Molecular , Schistosoma mansoni/efeitos dos fármacos , Esquistossomose mansoni/enzimologia , Relação Estrutura-Atividade
3.
Biochim Biophys Acta ; 1844(7): 1317-31, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24721563

RESUMO

Bovine CD38/NAD(+) glycohydrolase catalyzes the hydrolysis of NAD(+) to nicotinamide and ADP-ribose and the formation of cyclic ADP-ribose via a stepwise reaction mechanism. Our recent crystallographic study of its Michaelis complex and covalently-trapped intermediates provided insights into the modalities of substrate binding and the molecular mechanism of bCD38. The aim of the present work was to determine the precise role of key conserved active site residues (Trp118, Glu138, Asp147, Trp181 and Glu218) by focusing mainly on the cleavage of the nicotinamide-ribosyl bond. We analyzed the kinetic parameters of mutants of these residues which reside within the bCD38 subdomain in the vicinity of the scissile bond of bound NAD(+). To address the reaction mechanism we also performed chemical rescue experiments with neutral (methanol) and ionic (azide, formate) nucleophiles. The crucial role of Glu218, which orients the substrate for cleavage by interacting with the N-ribosyl 2'-OH group of NAD(+), was highlighted. This contribution to catalysis accounts for almost half of the reaction energy barrier. Other contributions can be ascribed notably to Glu138 and Asp147 via ground-state destabilization and desolvation in the vicinity of the scissile bond. Key interactions with Trp118 and Trp181 were also proven to stabilize the ribooxocarbenium ion-like transition state. Altogether we propose that, as an alternative to a covalent acylal reaction intermediate with Glu218, catalysis by bCD38 proceeds through the formation of a discrete and transient ribooxocarbenium intermediate which is stabilized within the active site mostly by electrostatic interactions.


Assuntos
ADP-Ribosil Ciclase 1/metabolismo , Aminoácidos/genética , Mutação/genética , NAD/metabolismo , ADP-Ribosil Ciclase 1/química , ADP-Ribosil Ciclase 1/genética , Animais , Catálise , Domínio Catalítico , Bovinos , Glicosilação , Hidrólise , Cinética , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Mutagênese Sítio-Dirigida , Especificidade por Substrato
4.
Biochim Biophys Acta ; 1834(12): 2520-7, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24035885

RESUMO

Schistosoma mansoni NAD(+) catabolizing enzyme (SmNACE), a distant homolog of mammalian CD38, shows significant structural and functional analogy to the members of the CD38/ADP-ribosyl cyclase family. The hallmark of SmNACE is the lack of ADP-ribosyl cyclase activity that might be ascribed to subtle changes in its active site. To better characterize the residues of the active site we determined the kinetic parameters of nine mutants encompassing three acidic residues: (i) the putative catalytic residue Glu202 and (ii) two acidic residues within the 'signature' region (the conserved Glu124 and the downstream Asp133), (iii) Ser169, a strictly conserved polar residue and (iv) two aromatic residues (His103 and Trp165). We established the very important role of Glu202 and of the hydrophobic domains overwhelmingly in the efficiency of the nicotinamide-ribosyl bond cleavage step. We also demonstrated that in sharp contrast with mammalian CD38, the 'signature' Glu124 is as critical as Glu202 for catalysis by the parasite enzyme. The different environments of the two Glu residues in the crystal structure of CD38 and in the homology model of SmNACE could explain such functional discrepancies. Mutagenesis data and 3D structures also indicated the importance of aromatic residues, especially His103, in the stabilization of the reaction intermediate as well as in the selection of its conformation suitable for cyclization to cyclic ADP-ribose. Finally, we showed that inhibition of SmNACE by the natural product cyanidin requires the integrity of Glu202 and Glu124, but not of His103 and Trp165, hence suggesting different recognition modes for substrate and inhibitor.


Assuntos
Proteínas de Helminto/química , Hidrolases/química , Schistosoma mansoni/enzimologia , ADP-Ribosil Ciclase 1/química , ADP-Ribosil Ciclase 1/genética , Aminoácidos , Animais , Antocianinas/química , Domínio Catalítico , Proteínas de Helminto/genética , Humanos , Hidrolases/antagonistas & inibidores , Hidrolases/genética , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/genética , NAD/antagonistas & inibidores , NAD/química , NAD/genética , Estrutura Terciária de Proteína , Schistosoma mansoni/genética , Homologia Estrutural de Proteína
5.
PLoS One ; 7(4): e34918, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22529956

RESUMO

Bovine CD38/NAD(+)glycohydrolase (bCD38) catalyses the hydrolysis of NAD(+) into nicotinamide and ADP-ribose and the formation of cyclic ADP-ribose (cADPR). We solved the crystal structures of the mono N-glycosylated forms of the ecto-domain of bCD38 or the catalytic residue mutant Glu218Gln in their apo state or bound to aFNAD or rFNAD, two 2'-fluorinated analogs of NAD(+). Both compounds behave as mechanism-based inhibitors, allowing the trapping of a reaction intermediate covalently linked to Glu218. Compared to the non-covalent (Michaelis) complex, the ligands adopt a more folded conformation in the covalent complexes. Altogether these crystallographic snapshots along the reaction pathway reveal the drastic conformational rearrangements undergone by the ligand during catalysis with the repositioning of its adenine ring from a solvent-exposed position stacked against Trp168 to a more buried position stacked against Trp181. This adenine flipping between conserved tryptophans is a prerequisite for the proper positioning of the N1 of the adenine ring to perform the nucleophilic attack on the C1' of the ribofuranoside ring ultimately yielding cADPR. In all structures, however, the adenine ring adopts the most thermodynamically favorable anti conformation, explaining why cyclization, which requires a syn conformation, remains a rare alternate event in the reactions catalyzed by bCD38 (cADPR represents only 1% of the reaction products). In the Michaelis complex, the substrate is bound in a constrained conformation; the enzyme uses this ground-state destabilization, in addition to a hydrophobic environment and desolvation of the nicotinamide-ribosyl bond, to destabilize the scissile bond leading to the formation of a ribooxocarbenium ion intermediate. The Glu218 side chain stabilizes this reaction intermediate and plays another important role during catalysis by polarizing the 2'-OH of the substrate NAD(+). Based on our structural analysis and data on active site mutants, we propose a detailed analysis of the catalytic mechanism.


Assuntos
ADP-Ribosil Ciclase 1/química , ADP-Ribosil Ciclase/química , ADP-Ribosil Ciclase/metabolismo , ADP-Ribosil Ciclase 1/genética , ADP-Ribosil Ciclase 1/metabolismo , Monofosfato de Adenosina/química , Substituição de Aminoácidos , Animais , Catálise , Domínio Catalítico , Bovinos , Cristalografia por Raios X , Glicosilação , Ligantes , Modelos Moleculares , Mutagênese Sítio-Dirigida , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , NAD/análogos & derivados , NAD/química , NAD/metabolismo , Ligação Proteica , Multimerização Proteica , Estrutura Terciária de Proteína , Especificidade por Substrato
6.
Eur J Med Chem ; 51: 174-83, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22483966

RESUMO

A more complete understanding of the mechanism of action of TLR agonists has fueled the investigation of new synthetic immunoadjuvants. In this context, we designed and synthesized glycolipids of the type Pam(2)Cys-α-Galactose as novel immunoadjuvants. Their synthesis required modifying a hydrophobic tBoc-[2,3-bispalmitoyloxy-(2R)-propyl]-R-cysteinyl moiety, i.e. the minimal structure required for TLR2 agonist activity, by addition of a hydrophilic head, either an α-Galactosylpyranose or an α-Galactosylfuranose to gain respectively Pam(2)CGalp and Pam(2)CGalf. While preparing a carbohydrate building block, an unexpected stereoselectivity was observed during a halide ion-catalytic process on a protected galactofuranose: the alpha anomer was obtained with surprisingly high selectivity (α/ß ratio>9) and with good isolated yield (51%). The TLR2 binding properties of Pam(2)CGalp and Pam(2)CGalf were then fully evaluated. Their efficiency in triggering the proliferation of BALB/c mouse splenocytes was also compared to that of Pam(2)CAG and Pam(3)CAG, two well-established ligands of TLRs. Moreover, the maturation state of murine dendritic cells previously incubated with either Pam(2)CGalp or Pam(2)CGalf was monitored by flow cytometry and compared to that induced by lipopolysaccharide. Pam(2)CGalp and Pam(2)CGalf were found to be equivalent TLR2 agonists, and induced splenocyte proliferation and DC maturation. With very similar activity, Pam(2)CGalp and Pam(2)CGalf were also 10-fold to 100-fold better than Pam(2)CAG and Pam(3)CAG at inducing B cell proliferation. This represents the first time a glucidic head has been added to the tBoc-[2,3-bispalmitoyloxy-(2R)-propyl]-R-cysteinyl moiety whilst maintaining the immunomodulating activity. This should greatly enrich the data available on Pam(2)C structure/activity relationships.


Assuntos
Adjuvantes Imunológicos/química , Adjuvantes Imunológicos/metabolismo , Técnicas de Química Sintética , Galactose/química , Glicolipídeos/química , Glicolipídeos/metabolismo , Receptor 2 Toll-Like/metabolismo , Adjuvantes Imunológicos/síntese química , Adjuvantes Imunológicos/farmacologia , Animais , Linhagem Celular , Feminino , Glicolipídeos/síntese química , Glicolipídeos/farmacologia , Humanos , Ligantes , Camundongos , Relação Estrutura-Atividade , Receptor 2 Toll-Like/agonistas
7.
Bioorg Med Chem Lett ; 21(13): 3939-42, 2011 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-21641214

RESUMO

CD38 is a multifunctional enzyme which is ubiquitously distributed in mammalian tissues. It is involved in the conversion of NAD(P)(+) into cyclic ADP-ribose, NAADP(+) and ADP-ribose and the role of these metabolites in multiple Ca(2+) signaling pathways makes CD38 a novel potential pharmacological target. The dire paucity of CD38 inhibitors, however, renders the search for new molecular tools highly desirable. We report that human CD38 is inhibited at low micromolar concentrations by flavonoids such as luteolinidin, kuromanin and luteolin (IC(50) <10 µM). Docking studies provide some clues on the mode of interaction of these molecules with the active site of CD38.


Assuntos
ADP-Ribosil Ciclase 1/antagonistas & inibidores , Inibidores Enzimáticos/química , Flavonoides/farmacologia , Antocianinas/química , Antocianinas/farmacologia , Domínio Catalítico , Ativação Enzimática/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Flavonoides/química , Humanos , Concentração Inibidora 50 , Modelos Moleculares , Estrutura Molecular
8.
Biomaterials ; 32(20): 4574-83, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21474175

RESUMO

Synthetic and molecularly defined constructs containing the minimal components to mimic and amplify the physiological immune response are able to induce an efficient cytotoxic response. In the current study this approach was applied to the development of highly versatile liposomal constructs to co-deliver peptide epitopes in combination with TLR agonists in order to induce a specific anti-tumor cellular immune response against ErbB2 protein-expressing tumor cells. Liposomes containing ErbB2 p63-71 cytotoxic T lymphocyte (CTL) and HA307-319 T- helper (Th) peptide epitopes associated to innovative synthetic TLR2/1 (Pam(3)CAG) or TLR2/6 agonists (Pam(2)CAG and Pam(2)CGD), were injected in mice bearing ErbB2 protein-expressing tumor cells. Mannosylated ligands were also incorporated into the constructs to target antigen-presenting cells. We showed that the TLR2/6 agonists were more efficient than the TLR2/1 agonists for the eradication of tumors expressing ErbB2 protein. Furthermore, mannose-targeted liposomes displayed higher therapeutic efficiency against tumor allowing treatment with decreased quantities of both TLR ligands and peptide epitopes. Our results validated that antigen-associated mannosylated liposomes combined with efficient TLR ligands are effective vectors for vaccination against tumor. In this study we developed useful tools to evaluate the vaccination efficiency of various adjuvants and/or targeting molecules and their potential synergy.


Assuntos
Vacinas Anticâncer/uso terapêutico , Lectinas Tipo C/imunologia , Lipossomos/imunologia , Lectinas de Ligação a Manose/imunologia , Neoplasias , Peptídeos/imunologia , Receptor ErbB-2/imunologia , Receptores de Superfície Celular/imunologia , Receptores Toll-Like/agonistas , Adjuvantes Imunológicos/química , Animais , Epitopos/imunologia , Feminino , Lipossomos/química , Receptor de Manose , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Neoplasias/prevenção & controle , Peptídeos/química , Receptores Toll-Like/imunologia
9.
Bioorg Med Chem ; 18(22): 7900-10, 2010 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-20951593

RESUMO

Schistosomiasis is a major tropical parasitic disease. For its treatment, praziquantel remains the only effective drug available and the dependence on this sole chemotherapy emphasizes the urgent need for new drugs to control this neglected disease. In this context, the newly characterized Schistosoma mansoni NAD(+) catabolizing enzyme (SmNACE) represents a potentially attractive drug target. This potent NAD(+)glycohydrolase, which is localized to the outer surface (tegument) of the adult parasite, is presumably involved in the parasite survival by manipulating the host's immune regulatory pathways. In an effort to identify SmNACE inhibitors, we have developed a sensitive and robust fluorometric high-throughput screening assay. The implementation of this assay to the screening of a highly diverse academic chemical library of 14,300 molecules yielded, after secondary assays and generation of dose-response curves, the identification of two natural product inhibitors, cyanidin and delphinidin. These confirmed hits inhibit SmNACE with IC(50) values in the low micromolar range. To rationalize the structure-activity relationship, several related flavonoids were tested, thereby leading to the identification of 15 additional natural product inhibitors. A selection of representative flavonoid inhibitors indicated that although they also inhibit the homologous human CD38, a selectivity in favor of SmNACE could be reached. Docking studies indicated that these inhibitors mimic the binding mode of the enzyme substrate NAD(+) and suggested the pharmacophoric features required for SmNACE active site recognition.


Assuntos
Inibidores Enzimáticos/química , Flavonoides/química , NAD+ Nucleosidase/química , Schistosoma mansoni/enzimologia , Esquistossomicidas/química , ADP-Ribosil Ciclase 1/antagonistas & inibidores , ADP-Ribosil Ciclase 1/metabolismo , Animais , Sítios de Ligação , Domínio Catalítico , Simulação por Computador , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/farmacologia , Flavonoides/síntese química , Flavonoides/farmacologia , Ensaios de Triagem em Larga Escala , Humanos , NAD+ Nucleosidase/metabolismo , Esquistossomicidas/síntese química , Esquistossomicidas/farmacologia , Relação Estrutura-Atividade
10.
J Biol Chem ; 285(26): 19900-9, 2010 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-20385553

RESUMO

Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate are ubiquitous calcium-mobilizing messengers produced by the same family of multifunctional enzymes, the ADP-ribosyl cyclases. Not all ADP-ribosyl cyclases have been identified, and how production of different messengers is achieved is incompletely understood. Here, we report the cloning and characterization of a novel ADP-ribosyl cyclase (SpARC4) from the sea urchin, a key model organism for the study of calcium-signaling pathways. Like several other members of the ADP-ribosyl cyclase superfamily, SpARC4 is a glycoprotein targeted to the plasma membrane via a glycosylphosphatidylinositol anchor. However, unlike most other members, SpARC4 shows a remarkable preference for producing cyclic ADP-ribose over nicotinic acid adenine dinucleotide phosphate. Mutation of a single residue (tyrosine 142) within a noncanonical active site reversed this striking preference. Our data highlight further diversification of this unusual enzyme family, provide mechanistic insight into multifunctionality, and suggest that different ADP-ribosyl cyclases are fine-tuned to produce specific calcium-mobilizing messengers.


Assuntos
ADP-Ribosil Ciclase/metabolismo , ADP-Ribose Cíclica/metabolismo , NADP/análogos & derivados , ADP-Ribosil Ciclase/genética , Sequência de Aminoácidos , Animais , Blastômeros/citologia , Blastômeros/metabolismo , Western Blotting , Linhagem Celular , Clonagem Molecular , Humanos , Cinética , Microinjeções , Microscopia Confocal , Dados de Sequência Molecular , Mutação , NADP/metabolismo , Homologia de Sequência de Aminoácidos , Strongylocentrotus purpuratus/enzimologia , Strongylocentrotus purpuratus/genética , Strongylocentrotus purpuratus/metabolismo , Transfecção , Tirosina/genética , Tirosina/metabolismo , Xenopus laevis/embriologia
11.
Methods Mol Biol ; 605: 267-77, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20072887

RESUMO

Click chemistry represents a new bioconjugation strategy that can be used to conveniently attach various ligands to the surface of preformed liposomes. This efficient and chemoselective reaction involves a Cu(I)-catalyzed azide-alkyne cycloaddition, which can be performed under mild experimental conditions in aqueous media. Here, we describe the application of a model click reaction to the conjugation, in a single step of unprotected alpha-1-thiomannosyl ligands, functionalized with an azide group to liposomes containing a terminal alkyne-functionalized lipid anchor. Excellent coupling yields were obtained in the presence of bathophenanthrolinedisulphonate, a water soluble copper-ion chelator, acting as a catalyst. No vesicle leakage was triggered by this conjugation reaction and the coupled mannose ligands were exposed at the surface of the liposomes. The major limitation of Cu(I)-catalyzed click reactions is that this conjugation is restricted to liposomes made of saturated (phospho)lipids. Efficient copper-free azide-alkyne click reactions are, however, being developed, which should alleviate this constraint in the future.


Assuntos
Alcinos/química , Azidas/química , Azidas/síntese química , Lipossomos/química , Manose/química , Alcinos/síntese química , Cobre/química , Ciclização , Ligantes , Manose/síntese química , Propriedades de Superfície
12.
Protein Expr Purif ; 70(2): 151-7, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19818407

RESUMO

Bovine CD38, a type II glycoprotein, contains two potential N-glycosylation sites (Asn-201 and Asn-268) in its extracellular domain. This contrasts with the other mammalian members of the ADP-ribosyl cyclase family, such as human CD38 and BST-1/CD157, in which four such sites are present. Our study was designed to determine the occupancy of these sites in a recombinant form of this ecto-enzyme and to evaluate its impact on the protein stability and catalytic functions. To that end we have successfully expressed the hydrosoluble ecto-domain of bovine CD38 (bCD38; residues 32-278), and corresponding glycosylation mutants, in the methylotrophic yeast Pichia pastoris. The secreted proteins were purified to homogeneity by affinity chromatography on immobilized Cibacron blue. We found by site-directed mutagenesis and mass spectrometry that bCD38 was a monoglycosylated protein at Asn-201. The expression yield of the deglycosylated mutants was not significantly affected, indicating that glycosylation at Asn-201 was not required for a proper processing and secretion of this protein by P. pastoris. Significant alterations in the kinetic parameters of NAD(+) were observed for the deglycosylated mutants. The thermostability of the recombinant enzyme was also influenced by mutation at position 201. Interestingly both parameters were dependent on the nature of the mutant and a stable deglycosylated mutant N201D of bCD38 could be produced that can be further used for structural studies.


Assuntos
ADP-Ribosil Ciclase 1/química , ADP-Ribosil Ciclase 1/genética , ADP-Ribosil Ciclase 1/biossíntese , Sequência de Aminoácidos , Animais , Catálise , Bovinos , Estabilidade Enzimática , Glicosilação , Cinética , Mutagênese Sítio-Dirigida , Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/metabolismo , Pichia/genética , Pichia/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética
13.
Vaccine ; 27(39): 5419-26, 2009 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-19559116

RESUMO

We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of the Shigella flexneri 2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307-319 (Th epitope). Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam(3)CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti-S. flexneri 2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against a S. flexneri 2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.


Assuntos
Vacinas Bacterianas/imunologia , Lipossomos/imunologia , Antígenos O/imunologia , Oligossacarídeos/imunologia , Shigella flexneri/imunologia , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/imunologia , Disenteria Bacilar/imunologia , Disenteria Bacilar/prevenção & controle , Epitopos de Linfócito B/imunologia , Epitopos de Linfócito T/imunologia , Feminino , Hemaglutininas/imunologia , Lipopeptídeos/química , Lipopeptídeos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Antígenos O/química , Oligossacarídeos/química , Orthomyxoviridae , Vacinas Sintéticas/imunologia
14.
Bioconjug Chem ; 19(12): 2385-93, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19053315

RESUMO

An important challenge for the development of new generations of vaccines is the efficient delivery of antigens to antigen presenting cells such as dendritic cells. In the present study we compare the interaction of plain and targeted liposomes, containing mono-, di-, and tetraantennary mannosyl lipid derivatives, with human monocyte-derived immature dendritic cells (iDCs). Whereas efficient mannose receptor-mediated endocytosis by iDCs was observed for the mannosylated liposomes, in contrast, only nonspecific interaction with little uptake was observed with plain liposomes. In accordance with the clustering effect, liposomes prepared with multibranched mannosylated lipids displayed higher binding affinity for the mannose receptor than vesicles containing the monomannosylated analogs. Importantly, we have found that dimannosylated ligands present at the surface of the liposomes were as efficient as tetramannosylated ones to engage in multidentate interactions with the mannose receptor of iDCs, resulting in both cases in an effective uptake/endocytosis. This result will greatly facilitate, from a practical standpoint, the design of mannose-targeted vaccination constructs. Moreover, we showed that mannose-mediated uptake of liposomes did not result in an activation of iDCs. Altogether, our results suggest that antigen-associated targeted liposomes containing diantennary mannosylated lipids could be effective vectors for vaccines when combined with additional DC activation signals.


Assuntos
Células Dendríticas/metabolismo , Lipossomos/química , Lipossomos/metabolismo , Manose/química , Sobrevivência Celular/efeitos dos fármacos , Ácido Clodrônico/química , Células Dendríticas/citologia , Células Dendríticas/efeitos dos fármacos , Endocitose , Fluoresceínas/química , Humanos , Espaço Intracelular/metabolismo , Ligantes , Lipossomos/síntese química , Lipossomos/toxicidade
15.
Biochemistry ; 46(13): 4100-9, 2007 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-17341094

RESUMO

ADP-ribosyl cyclases catalyze the transformation of nicotinamide adenine dinucleotide (NAD+) into the calcium-mobilizing nucleotide second messenger cyclic adenosine diphosphoribose (cADP-ribose) by adenine N1-cyclization onto the C-1' ' position of NAD+. The invertebrate Aplysia californica ADP-ribosyl cyclase is unusual among this family of enzymes by acting exclusively as a cyclase, whereas the other members, such as CD38 and CD157, also act as NAD+ glycohydrolases, following a partitioning kinetic mechanism. To explore the intramolecular cyclization reaction, the novel nicotinamide 2-fluoroadenine dinucleotide (2-fluoro-NAD+) was designed as a sterically very close analogue to the natural substrate NAD+, with only an electronic perturbation at the critical N1 position of the adenine base designed to impede the cyclization reaction. 2-Fluoro-NAD+ was synthesized in high yield via Lewis acid catalyzed activation of the phosphoromorpholidate derivative of 2-fluoroadenosine 5'-monophosphate and coupling with nicotinamide 5'-monophosphate. With 2-fluoro-NAD+ as substrate, A. californica ADP-ribosyl cyclase exhibited exclusively a NAD+ glycohydrolase activity, catalyzing its hydrolytic transformation into 2-fluoro-ADP-ribose, albeit at a rate ca. 100-fold slower than for the cyclization of NAD+ and also, in the presence of methanol, into its methanolysis product beta-1' '-O-methyl 2-fluoro-ADP-ribose with a preference for methanolysis over hydrolysis of ca. 100:1. CD38 likely converted 2-fluoro-NAD+ exclusively into the same product. We conclude that A. californica ADP-ribosyl cyclase can indeed be classified as a multifunctional enzyme that also exhibits a classical NAD+ glycohydrolase function. This alternative pathway that remains, however, kinetically cryptic when using NAD+ as substrate can be unmasked with a dinucleotide analogue whose conversion into the cyclic derivative is blocked. 2-Fluoro-NAD+ is therefore a useful molecular tool allowing dissection of the kinetic scheme for this enzyme.


Assuntos
ADP-Ribosil Ciclase 1/metabolismo , NAD+ Nucleosidase/metabolismo , NAD/análogos & derivados , Animais , Aplysia/enzimologia , Simulação por Computador , Cinética , Modelos Químicos , Modelos Moleculares , NAD/síntese química , NAD/química , NAD/metabolismo
16.
Biochemistry ; 45(39): 11867-78, 2006 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-17002287

RESUMO

Schistosoma mansoni NAD(P)+ catabolizing enzyme (SmNACE) is a new member of the ADP-ribosyl cyclase family. In contrast to all the other enzymes that are involved in the production of metabolites that elicit Ca2+ mobilization, SmNACE is virtually unable to transform NAD+ into the second messenger cyclic ADP-ribose (cADPR). Sequence alignments revealed that one of four conserved residues within the active site of these enzymes was replaced in SmNACE by a histidine (His103) instead of the highly conserved tryptophan. To find out whether the inability of SmNACE to catalyze the canonical ADP-ribosyl cyclase reaction is linked to this change, we have replaced His103 with a tryptophan. The H103W mutation in SmNACE was indeed found to restore ADP-ribosyl cyclase activity as cADPR amounts for 7% of the reaction products (i.e., a value larger than observed for other members of this family such as CD38). Introduction of a Trp103 residue provides some of the binding characteristics of mammalian ADP-ribosyl cyclases such as increased affinity for Cibacron blue and slow-binding inhibition by araF-NAD+. Homology modeling of wild-type and H103W mutant three-dimensional structures, and docking of substrates within the active sites, provides new insight into the catalytic mechanism of SmNACE. Both residue side chains share similar roles in the nicotinamide-ribose bond cleavage step leading to an E.ADP-ribosyl reaction intermediate. They diverge, however, in the evolution of this intermediate; His103 provides a more polar environment favoring the accessibility to water and hydrolysis leading to ADP-ribose at the expense of the intramolecular cyclization pathway resulting in cADPR.


Assuntos
ADP-Ribosil Ciclase/genética , Proteínas de Helminto/genética , Mutação de Sentido Incorreto , NAD/metabolismo , Schistosoma mansoni/genética , ADP-Ribosil Ciclase/metabolismo , ADP-Ribosil Ciclase 1/genética , ADP-Ribosil Ciclase 1/metabolismo , Substituição de Aminoácidos , Animais , Sítios de Ligação/genética , Cálcio/metabolismo , Catálise , ADP-Ribose Cíclica/genética , ADP-Ribose Cíclica/metabolismo , Proteínas de Helminto/metabolismo , Humanos , Schistosoma mansoni/enzimologia
17.
J Liposome Res ; 16(3): 205-13, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16952875

RESUMO

We have developed liposome-based synthetic constructs incorporating peptide epitope(s) (ErbB2 p63-67 CTL which is overexpressed in many tumors and/or HA 307-319 T-helper) and lipopeptide adjuvants (Pam3CysSerSer, Pam3CysAlaGly) in order to elicit an anti-tumor immune response. The epitopes, derivatized with a linker containing a cysteine residue, were conjugated on preformed vesicles (dia. approximately 100 nm) containing lipopeptides functionalized with thiol reactive groups (maleimide or bromoacetyl). The therapeutic efficacy of these constructs was evaluated on a Balb/c mice tumor model inoculated with syngenic murine renal carcinoma (Renca) cells expressing human ErbB2 (Her2/neu) receptor. A successful therapeutic vaccination was obtained which was antigen specific. Furthermore, it appeared that the nature of the polar head group of the lipopeptide adjuvant and also its type of functionalization influence the efficacy of the construct. In our study, the best results were obtained with formulations containing a Pam3CSS anchor in association with the CTL and Th epitopes. Considering these promising results studies are in progress with a new generation of liposomes that incorporate a neutral lipid--lacking adjuvant properties--that serves as anchor of the peptide epitopes and new adjuvants synthesized in our laboratory, which are screened for their antitumour activity in a therapeutic setting.


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Vacinas Anticâncer/administração & dosagem , Neoplasias Renais/terapia , Lipossomos , Peptídeos/administração & dosagem , Adjuvantes Imunológicos/química , Sequência de Aminoácidos , Animais , Camundongos , Dados de Sequência Molecular , Peptídeos/química
18.
J Liposome Res ; 16(3): 229-35, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16952877

RESUMO

DNA vaccination with mammalian-expressible plasmid DNA encoding protein antigens is known to be an effective means to elicit cell-mediated immunity, sometimes in the absence of a significant antibody response. This may be contrasted with protein vaccination, which gives rise to antibody responses with little evidence of cell-mediated immunity. This has led to considerable interest in DNA vaccination as a means to elicit cell-mediated immune responses against conserved viral antigens or intracellular cancer antigens, for the purpose of therapeutic vaccination. However, almost all current vaccines are used prophylactically and work by producing antibodies rather than cell mediated immune responses. In the present study we have therefore explored the combination of DNA and protein forms of an antigen using two exemplary prophylactic vaccine antigens, namely inactivated influenza virion and hepatitis-B surface antigen. We studied the effects of various combinations of DNA and protein on the antibody response. Co-administration of soluble forms of DNA and protein representations of the same antigen gave rise to the same level of antibody response as if protein were administered alone. In contrast, we found that when these antigens are entrapped in the same liposomal compartment, that there was a strong synergistic effect on the immune response, which was much greater than when either antigen was administered alone, or in various other modes of combination (e.g. co-administration as free entities, also pooled liposomal formulations where the two materials were contained in separate liposomal vehicles in the same suspension). The synergistic effect of liposomally co-entrapped DNA and protein exceeded, markedly, the well known adjuvant effects of plasmid DNA and liposomes. We have termed this new approach to vaccination 'co-delivery' and suggest that it may derive from the simultaneous presentation of antigen via MHC class-I (DNA) and MHC class-II (protein) pathways to CD8+ and CD4+ cells at the same antigen presenting cell--a mode of presentation that would commonly occur with live viral pathogens. We conclude that co-delivery is a very effective means to generate protective antibody responses against viral pathogens.


Assuntos
Vacinas contra Hepatite B , Vacinas contra Influenza , Lipossomos , Vacinas contra Hepatite B/administração & dosagem , Vacinas contra Hepatite B/imunologia , Humanos , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/imunologia
19.
Bioconjug Chem ; 17(3): 849-54, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16704226

RESUMO

An efficient and convenient chemoselective conjugation method based on "click chemistry" was developed for coupling ligands to the surface of preformed liposomes. It can be performed under mild conditions in aqueous buffers; the use of a water soluble Cu(I) chelator, such as bathophenanthrolinedisulfonate, was essential to obtain good yields in reasonable reaction times. A model reaction was achieved in which, in a single step, an unprotected alpha-D-mannosyl derivative carrying a spacer arm functionalized with an azide group was conjugated to the surface of vesicles presenting a synthetic lipid carrying a terminal alkyne function. When liposomes composed of saturated phospholipids were used, the reaction conditions developed in the present work did not damage the membranes as measured by the absence of leakage of entrapped 5,6-carboxyfluorescein. Moreover, as assessed by agglutination experiments using concanavalin A, the mannose residues were perfectly accessible on the surface of the targeted vesicles.


Assuntos
Lipossomos/química , Modelos Químicos , Azidas/química , Ligantes , Manose/química
20.
Int Immunol ; 18(7): 1029-42, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16720618

RESUMO

Cross-linking of CD38 on hematopoietic cells induces activation, proliferation and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). It has been previously postulated that the calcium-mobilizing metabolites produced by CD38 may regulate its receptor-based activities. To test this hypothesis, we examined whether the enzyme activity of CD38 controls the apoptosis of an anti-CD38-stimulated leukemic B cell. We show that anti-CD38-induced apoptosis of Ba/F3 cells, a murine pro-B cell line, is not affected by blocking the calcium-mobilizing activity of cADPR or by inhibiting intracellular or extracellular calcium mobilization. In addition, we demonstrate that blocking CD38 enzyme activity with 2'-deoxy-2'-fluoro-nicotinamide arabinoside adenine dinucleotide has no effect on apoptosis and that Ba/F3 cells expressing catalytically inactive mutant forms of CD38 still undergo apoptosis upon CD38 cross-linking. Instead, we find that anti-CD38-induced apoptosis is dependent on tyrosine kinase and caspase activation, and that this process appears to be potentiated by the presence of membrane microdomains. Thus, the receptor-mediated functions of CD38 can be separated from its enzyme activity in a murine leukemic cell line, suggesting that CD38 plays multiple, but independent, biologic roles.


Assuntos
ADP-Ribosil Ciclase 1/imunologia , ADP-Ribosil Ciclase/imunologia , Apoptose/imunologia , Linfócitos B/imunologia , Sinalização do Cálcio/imunologia , ADP-Ribosil Ciclase/antagonistas & inibidores , ADP-Ribosil Ciclase/metabolismo , ADP-Ribosil Ciclase 1/antagonistas & inibidores , ADP-Ribosil Ciclase 1/metabolismo , Animais , Apoptose/efeitos dos fármacos , Linfócitos B/enzimologia , Cálcio/metabolismo , Sinalização do Cálcio/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/imunologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , ADP-Ribose Cíclica/imunologia , ADP-Ribose Cíclica/metabolismo , Inibidores Enzimáticos/farmacologia , Células-Tronco Hematopoéticas/imunologia , Células-Tronco Hematopoéticas/metabolismo , Capeamento Imunológico/efeitos dos fármacos , Capeamento Imunológico/imunologia , Leucemia de Células B/imunologia , Leucemia de Células B/metabolismo , Ativação Linfocitária/efeitos dos fármacos , Ativação Linfocitária/imunologia , Microdomínios da Membrana/enzimologia , Microdomínios da Membrana/imunologia , Camundongos , NAD/análogos & derivados , NAD/farmacologia
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