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J Virol Methods ; 287: 114003, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33164863

RESUMO

The last major Rift Valley fever outbreak in South Africa was between 2008 and 2011. Viruses isolated between 2008 and 2010 were phylogenetically assigned to Lineage C, Lineage K and the novel lineage H. The 2011 outbreaks occurred primarily in the Eastern, Western and Northern Cape provinces, with no sequence data or phylogenetic relationship published. Samples from these outbreaks were submitted to the Faculty of Veterinary Sciences, University of Pretoria, for immunohistochemical confirmation of Rift Valley fever phlebovirus presence. These samples were formalin-fixed and paraffin-embedded (FFPE) and stored at the Pathology section for several years. This study describes a modified extraction method used to obtain RNA from the FFPE samples, as well as the primer combinations used to phylogenetically classify them as belonging to the novel lineage H.


Assuntos
Febre do Vale de Rift , Vírus da Febre do Vale do Rift , Animais , Surtos de Doenças , Formaldeído , Inclusão em Parafina , Filogenia , Estudos Retrospectivos , Febre do Vale de Rift/epidemiologia , Vírus da Febre do Vale do Rift/genética , África do Sul/epidemiologia
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