RESUMO
AIM: Study of intra-erythrocyte invasion of Escherichia coli strains with various levels of anti-hemoglobin activity on a model of generalized infection. MATERIALS AND METHODS: Experiments were carried out on 72 male mice. Generalized infection was induced by intravenous administration of E. coli strain day culture suspension with various values of hemolytic and anti-hemoglobin activity. The levels of hemoglobin in animal blood was determined by hemoglobin-cyanide method. Intra-erythrocyte invasion of E.coli was studied by using laser scanning confocal microscopy (LSCM). RESULTS: The largest decline (p < 0.05) of hemoglobin levels was detected in animals, infected with E. coli strains with a high levels of antiHbA and HA (58.3 ± 0.6 g/l and 69.7 ± 1.1 g/l). Using LSCM, microogranisms with a high levels of antiHbA were established to penetrate erythrocytes more frequently (70 ± 3.4%), than with a low (5.5 ± 1.7%). CONCLUSION: The largest frequency of bacteria detection in erythrocytes and the highest decline of blood hemoglobin levels were noted during infection of animals with E. coli strains with high anti-hemoglobin activity.
