RESUMO
Warts can be difficult to diagnose and to treat in the setting of human immunodeficiency virus (HIV) infection. A 37-year-old woman with a background of HIV presented with a large verrucous plaque involving her right foot. Human papillomavirus (HPV)-66 was identified in the lesional skin biopsy sample and in scrapings obtained from her cervix. The wart rapidly responded to topical cidofovir therapy. HPV-66 is a novel HPV type to be associated with verruca vulgaris. Topical cidofovir should be further investigated as an alternative treatment modality for verruca vulgaris.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/virologia , Antivirais/administração & dosagem , Citosina/análogos & derivados , Dermatoses do Pé/virologia , Organofosfonatos , Compostos Organofosforados/administração & dosagem , Papillomaviridae , Infecções por Papillomavirus/virologia , Verrugas/virologia , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Infecções Oportunistas Relacionadas com a AIDS/patologia , Administração Tópica , Adulto , Biópsia , Cidofovir , Citosina/administração & dosagem , DNA Viral/genética , Diagnóstico Diferencial , Feminino , Dermatoses do Pé/tratamento farmacológico , Dermatoses do Pé/patologia , Humanos , Masculino , Papillomaviridae/efeitos dos fármacos , Papillomaviridae/genética , Infecções por Papillomavirus/tratamento farmacológico , Infecções por Papillomavirus/patologia , Reação em Cadeia da Polimerase , Pele/patologia , Pele/virologia , Verrugas/tratamento farmacológico , Verrugas/patologiaAssuntos
Linfócitos T CD8-Positivos/imunologia , Proteína do Núcleo p24 do HIV/biossíntese , HIV-1/fisiologia , Neuroglia/virologia , Neurônios/virologia , Encéfalo/citologia , Linfócitos T CD4-Positivos/imunologia , Células Cultivadas , Técnicas de Cocultura , HIV-1/imunologia , Humanos , Leucócitos Mononucleares/imunologiaRESUMO
Antibodies of the IgM class and IgG2 and IgA2 subclasses are prominent in responses to pneumococcal polysaccharides (PPS) but may be decreased in human immunodeficiency virus (HIV)-infected patients, among whom invasive pneumococcal disease is common. After immunization of HIV-infected and -seronegative subjects with pneumococcal vaccine, the number of PPS-specific antibody-secreting cells (ASC) producing IgM was significantly lower among HIV-infected subjects, whereas PPS-specific IgG and IgA ASC were more comparable. The subclass distribution of PPS-specific IgG2-producing (approximately 80%) and IgA2-producing (approximately 50%) ASC and antibodies in serum were similar. However, before immunization, the proportions of PPS-specific IgG2 for both serotypes 8 and 14 in baseline sera from HIV-infected patients were significantly decreased compared with controls. Thus, the response to PPS among HIV-infected patients may be characterized by lower levels of specific IgG2 before immunization and prominent defects in IgM responses soon after stimulation.
Assuntos
Vacinas Bacterianas/imunologia , Infecções por HIV/imunologia , Imunoglobulina A/biossíntese , Imunoglobulina G/biossíntese , Imunoglobulina M/biossíntese , Streptococcus pneumoniae/imunologia , Vacinação , Adulto , Anticorpos Antibacterianos/classificação , Células Produtoras de Anticorpos/imunologia , Linfócitos B/imunologia , Estudos de Casos e Controles , Feminino , Soronegatividade para HIV/imunologia , Humanos , Ativação Linfocitária , Masculino , Vacinas PneumocócicasRESUMO
The pathogenesis of encephalitis due to cytomegalovirus (CMV), particularly the role of microglial cells in the spread or control of infection, remains incompletely defined. In this study, microglial cells were isolated from the brains of newborn mice and infected in vitro with murine CMV (MCMV). Microglial cells supported productive MCMV replication, and the MCMV-infected microglia manifested a cytopathic effect (CPE) characteristic of CMV infection. Exposure of microglia to interferon-gamma (IFN-gamma) 24 h before infection markedly suppressed virus production and resultant CPE in a dose-dependent fashion. Furthermore, the addition of IFN-gamma 2 h after infection demonstrated an antiviral effect equivalent to that achieved when IFN-gamma was administered 2 h before infection. These results demonstrate that murine microglial cells are fully permissive to MCMV replication and that IFN-gamma markedly suppresses virus expression in these cells.
