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1.
Appl Environ Microbiol ; 87(5): e0266220, 2021 02 12.
Artigo em Inglês | MEDLINE | ID: mdl-33355100

RESUMO

Currently, only 5 (SEA to SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits. Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc), which is part of the Staphylococcus aureus genomic island vSaß. These enterotoxins have been described as likely being involved in staphylococcal food-poisoning outbreaks. The aim of the present study was to determine if whole-genome data can be used for the prediction of staphylococcal egc enterotoxin production, particularly enterotoxin G (SEG) and enterotoxin I (SEI). For this purpose, whole-genome sequences of 75 Staphylococcus aureus strains from different origins (food-poisoning outbreaks, human, and animal) were investigated by applying bioinformatics methods (phylogenetic analysis using the core genome and different alignments). SEG and SEI expression was tested in vitro using a sandwich enzyme-linked immunosorbent assay method. Strains could be allocated to 14 different vSaß types, each type being associated with a single clonal complex (CC). In addition, the vSaß type and CC were associated with the origin of the strain (human or cattle derived). The amount of SEG and SEI produced also correlated with the vSaß type and the CC of a strain. The present results show promising indications that the in vitro production of SEG and SEI can be predicted based on the vSaß type or CC of a strain. IMPORTANCE Besides having infectious properties in human and animals, S. aureus can produce different enterotoxins in food. The enterotoxins can cause vomiting and diarrhea, often involving many people. Most of these outbreaks remain undiscovered, as detection methods for enterotoxins are only available for a few enterotoxins but not for the more recently discovered enterotoxins G (SEG) and I (SEI). In this study, we show promising results that in vitro production of SEG and SEI can be predicted based on the whole-genome sequencing data of a strain. In addition, these data could be used to find the source (human or cattle derived) of an outbreak strain, which is the key for a better understanding of the role SEG and SEI play in foodborne outbreaks caused by S. aureus.


Assuntos
Enterotoxinas , Doenças Transmitidas por Alimentos , Staphylococcus aureus , Animais , Técnicas de Tipagem Bacteriana , Bovinos , Enterotoxinas/genética , Doenças Transmitidas por Alimentos/epidemiologia , Humanos , Família Multigênica , Filogenia , Staphylococcus aureus/classificação , Staphylococcus aureus/genética
2.
Neuropsychopharmacology ; 46(3): 579-602, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-32781459

RESUMO

Maternal immune activation (MIA) and poor maternal nutritional habits are risk factors for the occurrence of neurodevelopmental disorders (NDD). Human studies show the deleterious impact of prenatal inflammation and low n-3 polyunsaturated fatty acid (PUFA) intake on neurodevelopment with long-lasting consequences on behavior. However, the mechanisms linking maternal nutritional status to MIA are still unclear, despite their relevance to the etiology of NDD. We demonstrate here that low maternal n-3 PUFA intake worsens MIA-induced early gut dysfunction, including modification of gut microbiota composition and higher local inflammatory reactivity. These deficits correlate with alterations of microglia-neuron crosstalk pathways and have long-lasting effects, both at transcriptional and behavioral levels. This work highlights the perinatal period as a critical time window, especially regarding the role of the gut-brain axis in neurodevelopment, elucidating the link between MIA, poor nutritional habits, and NDD.


Assuntos
Ácidos Graxos Ômega-3 , Efeitos Tardios da Exposição Pré-Natal , Animais , Comportamento Animal , Encéfalo , Feminino , Humanos , Inflamação , Microglia , Gravidez
3.
Cell Death Dis ; 2: e203, 2011 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-21881605

RESUMO

Brain protection of the newborn remains a challenging priority and represents a totally unmet medical need. Pharmacological inhibition of caspases appears as a promising strategy for neuroprotection. In a translational perspective, we have developed a pentapeptide-based group II caspase inhibitor, TRP601/ORPHA133563, which reaches the brain, and inhibits caspases activation, mitochondrial release of cytochrome c, and apoptosis in vivo. Single administration of TRP601 protects newborn rodent brain against excitotoxicity, hypoxia-ischemia, and perinatal arterial stroke with a 6-h therapeutic time window, and has no adverse effects on physiological parameters. Safety pharmacology investigations, and toxicology studies in rodent and canine neonates, suggest that TRP601 is a lead compound for further drug development to treat ischemic brain damage in human newborns.


Assuntos
Inibidores de Caspase , Inibidores de Cisteína Proteinase/uso terapêutico , Hipóxia-Isquemia Encefálica/tratamento farmacológico , Isquemia/tratamento farmacológico , Fármacos Neuroprotetores/uso terapêutico , Oligopeptídeos/uso terapêutico , Quinolinas/farmacologia , Animais , Animais Recém-Nascidos , Apoptose/efeitos dos fármacos , Sítios de Ligação , Caspases/metabolismo , Inibidores de Cisteína Proteinase/química , Citocromos c/metabolismo , Modelos Animais de Doenças , Hipóxia-Isquemia Encefálica/patologia , Isquemia/patologia , Camundongos , Fármacos Neuroprotetores/química , Oligopeptídeos/química , Oligopeptídeos/farmacologia , Quinolinas/química , Ratos
4.
Neuroscience ; 173: 156-68, 2011 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-21073926

RESUMO

Activity-dependent neuroprotective protein (ADNP) was shown to be essential for embryogenesis and brain development while NAP, an active motif of ADNP, is neuroprotective in a broad range of neurodegenerative disorders. In the present study, we examined the protective potential of ADNP/NAP in a mouse model of excitotoxic brain lesion mimicking brain damage associated with cerebral palsy. We demonstrated that NAP had a potent neuroprotective effect against ibotenate-induced excitotoxic damage in the cortical plate and the white matter of P5 mice, and moderate against brain lesions of P0 mice. In contrast, endogenous ADNP appears not to be involved in the response to excitotoxic challenge in the studied model. Our findings further show that NAP reduced the number of apoptotic neurons through activation of PI-3K/Akt pathway in the cortical plate or both PI-3K/Akt and MAPK/MEK1 kinases in the white matter. In addition, NAP prevented ibotenate-induced loss of pre-oligodendrocytes without affecting the number of astrocytes or activated microglia around the site of injection. These findings indicate that protective actions of NAP are mediated by triggering transduction pathways that are crucial for neuronal and oligodendroglial survival, thus, NAP might be a promising therapeutic agent for treating developing brain damage.


Assuntos
Lesões Encefálicas/prevenção & controle , Proteínas de Homeodomínio/farmacologia , Proteínas do Tecido Nervoso/farmacologia , Fármacos Neuroprotetores/farmacologia , Transdução de Sinais/efeitos dos fármacos , Animais , Animais Recém-Nascidos , Apoptose/efeitos dos fármacos , Western Blotting , Lesões Encefálicas/induzido quimicamente , Lesões Encefálicas/metabolismo , Paralisia Cerebral/metabolismo , Paralisia Cerebral/patologia , Modelos Animais de Doenças , Agonistas de Aminoácidos Excitatórios/toxicidade , Feminino , Proteínas de Homeodomínio/metabolismo , Ácido Ibotênico/toxicidade , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Masculino , Camundongos , Proteínas do Tecido Nervoso/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/patologia , Fármacos Neuroprotetores/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa
5.
J Mol Neurosci ; 13(1-2): 199-210, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10691306

RESUMO

Activity-dependent neurotrophic factor (ADNF) is a newly identified compound that prevents in vitro neuronal death when present in fentomolar concentrations. ADNF-14, a 14 amino acid peptide derived from ADNF, has the same effects on growth as the parent molecule. However, the transduction pathways and target cells for these highly potent trophic factors are still unknown. We previously described a mouse model of excitotoxic lesions of the developing neocortex mimicking several hypoxic or hypoxic-like brain lesions observed in human fetuses and neonates. In this model, cotreatment with the excitotoxin ibotenate and ADNF-14 prevented both neuronal death in pups injected on the day of birth and white matter cystic lesions in pups treated 5 d after birth. In the present study, coadministration of ibotenate, ADNF-14, and selective transduction pathway inhibitors showed that activation of protein kinase C (PKC) and mitogen-associated protein kinase kinase was critical for neuroprotection. Immunocytochemistry revealed that ADNF-14 activated PKC and mitogen-associated protein kinase in cortical neurons on the day of birth and in white matter astrocytes on the fifth postnatal day. Taken in concert, these data identify PKC and mitogen-associated protein kinase pathways as critical to ADNF-14-induced neuroprotection of the developing brain against excitotoxic damage.


Assuntos
Encéfalo/enzimologia , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fármacos Neuroprotetores/metabolismo , Fármacos Neuroprotetores/farmacologia , Neurotoxinas/metabolismo , Proteína Quinase C/metabolismo , Animais , Animais Recém-Nascidos , Astrócitos/metabolismo , Western Blotting , Encéfalo/citologia , Morte Celular , Camundongos , Proteínas do Tecido Nervoso/farmacologia , Neurônios/citologia , Neurônios/metabolismo , Neuropeptídeos , Oligopeptídeos
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