RESUMO
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
Assuntos
Núcleo Celular/ultraestrutura , Microscopia de Contraste de Fase/métodos , Algoritmos , Humanos , Processamento de Imagem Assistida por Computador/métodos , Síncrotrons , Difração de Raios X , Raios XRESUMO
We present a new method to control the power of individual spectral components of a multicolor laser by mirrors with variable air gaps and by a composite resonator configuration. We demonstrate a Pr/Yb-ZBLAN fiber laser with arbitrary spectral composition of three simultaneously emitted components at 492 nm, 520 nm, and 635 nm. With 100 mW pump power at 850 nm launched into the fiber, the total laser output exceeds 10 mW.
