Temporal coherence among tropical coastal lagoons: a search for patterns and mechanisms.

Temporal coherence (i.e., the degree of synchronicity of a given variable among ecological units within a predefined space) has been shown for several limnological features among temperate lakes, allowing predictions about the structure and function of ecosystems. However, there is little evidence of temporal coherence among tropical aquatic systems, where the climatic variability among seasons is less pronounced. Here, we used data from long-term monitoring of physical, chemical and biological variables to test the degree of temporal coherence among 18 tropical coastal lagoons. The water temperature and chlorophyll-a concentration had the highest and lowest temporal coherence among the lagoons, respectively, whereas the salinity and water colour had intermediate temporal coherence. The regional climactic factors were the main factors responsible for the coherence patterns in the water temperature and water colour, whereas the landscape position and morphometric characteristics explained much of the variation of the salinity and water colour among the lagoons. These results indicate that both local (lagoon morphometry) and regional (precipitation, air temperature) factors regulate the physical and chemical conditions of coastal lagoons by adjusting the terrestrial and marine subsidies at a landscape-scale. On the other hand, the chlorophyll-a concentration appears to be primarily regulated by specific local conditions resulting in a weak temporal coherence among the ecosystems. We concluded that temporal coherence in tropical ecosystems is possible, at least for some environmental features, and should be evaluated for other tropical ecosystems. Our results also reinforce that aquatic ecosystems should be studied more broadly to accomplish a full understanding of their structure and function.

Temporal coherence among tropical coastal lagoons: a search for patterns and mechanisms

Temporal coherence (i.e., the degree of synchronicity of a given variable among ecological units within a predefined space) has been shown for several limnological features among temperate lakes, allowing predictions about the structure and function of ecosystems. However, there is little evidence of temporal coherence among tropical aquatic systems, where the climatic variability among seasons is less pronounced. Here, we used data from long-term monitoring of physical, chemical and biological variables to test the degree of temporal coherence among 18 tropical coastal lagoons. The water temperature and chlorophyll-a concentration had the highest and lowest temporal coherence among the lagoons, respectively, whereas the salinity and water colour had intermediate temporal coherence. The regional climactic factors were the main factors responsible for the coherence patterns in the water temperature and water colour, whereas the landscape position and morphometric characteristics explained much of the variation of the salinity and water colour among the lagoons. These results indicate that both local (lagoon morphometry) and regional (precipitation, air temperature) factors regulate the physical and chemical conditions of coastal lagoons by adjusting the terrestrial and marine subsidies at a landscape-scale. On the other hand, the chlorophyll-a concentration appears to be primarily regulated by specific local conditions resulting in a weak temporal coherence among the ecosystems. We concluded that temporal coherence in tropical ecosystems is possible, at least for some environmental features, and should be evaluated for other tropical ecosystems. Our results also reinforce that aquatic ecosystems should be studied more broadly to accomplish a full understanding of their structure and function.

A coerência temporal (i.e., o nível de sincronismo de uma dada variável ecológica entre unidades ecológicas) tem sido demonstrada para uma vasta gama de variáveis limnológicas em lagos de clima temperado, permitindo que predições sobre a estrutura e o funcionamento destes ecossistemas sejam realizadas. Entretanto, há pouca evidência da coerência temporal de variáveis limnológicas entre ecossistemas aquáticos tropicais, onde a variação climática é menos pronunciada entre as estações do ano. Neste estudo, utilizamos dados de longa duração do monitoramento de variáveis físicas, químicas e biológicas para testar a ocorrência de coerência temporal entre 18 lagoas costeiras tropicais. A temperatura da água e a concentração de clorofila-a apresentaram, respectivamente, a maior e a menor coerência temporal entre as lagoas, enquanto que a salinidade e a coloração da água apresentaram padrões intermediários. Fatores climáticos regionais foram os principais fatores responsáveis pelos padrões de coerência da temperatura e coloração da água, enquanto que a orientação em relação ao mar e as características morfométricas das lagoas explicaram boa parte da variação da salinidade e da coloração da água entre as lagoas. Estes resultados indicam que tanto fatores locais (morfometria) quanto regionais (precipitação, temperatura do ar) regulam as condições físicas e químicas das lagoas costeiras pela contribuição dos ecossistemas terrestre e marinho em uma escala da paisagem. Por outro lado, a concentração de clorofila-a é aparentemente limitada por condições locais específicas, resultando em um fraco padrão de coerência temporal entre as lagoas. Concluímos que a coerência temporal em ecossistemas tropicais é possível, pelo menos para algumas variáveis ambientais, e deve ser avaliada para outros ecossistemas. Nossos resultados também reforçam a ideia que ecossistemas aquáticos devem ser estudados em uma perspectiva mais ampla (e.g. de bacia de drenagem) para o completo entendimento da sua estrutura e funcionamento.

Sperm as infection-potentiating cofactors in HIV transmission.

In this paper I will discuss the possible role of sperm as cofactors in the genital-mucosal transmission of HIV. The ideas involved arose from my laboratory's discovery that sperm bind to HLA-DR molecules expressed on somatic cells, and from our subsequent findings that lymphocytes are activated by these interactions. Sperm binding to HLA-DR mimics one of the two ligand binding characteristics of superantigens, which also bind to T-cell receptors in a V-beta-specific fashion. This property of sperm may be significant in HIV transmission because: (a) HLA-DR plays a central role in immune recognition and response; and (b) cell interactions involving HLA-DR are involved in HIV infection and disease development. After sexual contact, sperm elicit a transient leukocytic infiltration of the mammalian cervix (Thompson, L.A., Barratt, C.L., Bolton, A.E., Cooke, I.D., 1992. The leukocytic reaction of the human cervix. Am. J. Reprod. Immunol. 28, 85; Pandya, I.J., Cohen, J., 1985. The leukocytic reaction of the human uterine cervix to sperm. Fertil. Steril. 43, 417), and human cervical cells are bound and penetrated by sperm at this time (Sievers-Altermann, R., Engelbrecht, D.V., 1990. Entry of spermatozoa into the cervical mucosa and transmission of the AIDS virus. S. Afr. Med. J. 77, 319). At present, little is known about these in vivo events, but sperm-somatic cell interactions in vitro are also followed by sperm entry into the target cell cytoplasm and target cell activation. When the target cells are leukocytes, sperm interactions increase their susceptibility to HIV infection. If similar interactions occur in the cervicovaginal environment after sexual contact, they are likely to enhance the genital-mucosal transfer of HIV from semen.

Binding of sperm to somatic cells via HLA-DR. Modulation by sulfated carbohydrates.

We have shown previously that human sperm bind and enter leukocytes expressing surface HLA class II molecules. In the present study, mutant B lymphoblastoid cells and HLA-DR-transfected murine 3T3 fibroblasts are used to confirm that HLA class II molecules are somatic cell receptors for sperm. Further, for isolated HLA-DR expressed on murine cells, we show that sperm receptor activity requires the presence of sulfated carbohydrates. As carriers of multiple HLA-DR binding ligands, sperm may 1) mimic the target cell-activating effects of anti-DR antibody and 2) bind HIV through CD4-like or alternate receptors. By these or other mechanisms, sperm/somatic cell interactions in the female reproductive tract may affect fertility and potentiate the sexual transmission of AIDS.

Serum amyloid P component (SAP)-like protein from botryllid ascidians provides a clue to amyloid function.

The HA-1 lectin isolated from Botrylloides leachii has an amino acid composition similar to that of mammalian serum amyloid protein (SAP). SAP is a universal component of mammalian amyloid deposits. Like SAP, HA-1 has a disc ultrastructure, and antibody to HA-1 binds both (a) to amyloidlike fibers deposited between rejected Botrylloides colonies and (b) to cerebral amyloid deposits in Alzheimer's disease brains. Deposition of protochordate amyloid within rejection sites and surrounding fouling organisms implies that these fibers function as barriers to allogeneic and infectious challenge. Similarly, mammalian amyloid may also function to contain inflammatory lesions and to limit the spread of certain infections. Pathological amyloidotic conditions in humans, such as Alzheimer's disease, may result from unregulated expression of this primitive encapsulation response.

Sperm as activating cofactors in HIV sexual transmission.

PIP: Researchers at the University of California at Los Angeles and at the National Institute for Immunology in New Delhi, India conducted parallel laboratory studies which indicate that sperm may augment HIV infectivity. The studies showed that HIV binds easily to sperm. Further, sperm infects target peripheral blood leukocytes (PBLs) proficiently. In addition, sperm binds to HLA-DR molecules which can transduce signals. Similar to what happens when an immobilized anti HLA-DR antibody binds with the surface of HLA-DR which in turn activates HLA-DR expressing cells, sperm binding with HLA-DR also activates HLA-Dr expressing cells. The sperm/HLA-DR binding serves to transduce receptor coupled signals and expands and divides target cells. Target cell activation increases HIV entry and initiates latency. Furthermore new virus replication needs activation. If indeed sperm can activate HLA-DR bearing cells, this may explain both sperm increased HIV infectivity observed for PBLs in vitro and the efficiency of semen for transmitting HIV in vivo. Besides, HLA-DR bearing cells exist in great numbers in the female reproductive tract. In fact, some even express the HIV receptor CD4. These results suggest that sperm play an important role in the microenvironment leading to HIV infection. They also emphasize the significance of spermicide use with condoms to protect against HIV transmission.^ieng

Autoreactive blood cells and programmed cell death in growth and development of protochordates.

The tunicate Botryllus is a marine protochordate whose clonal colonies undergo regulated natural transplantations when they come into contact in nature. The outcome of these transplantations (fusion or rejection) is controlled by genes of a highly polymorphic histocompatibility system that resembles in many respects the mammalian major histocompatibility complex (MHC). While fusion or rejection reactions are often completed within 24 hr after transplantation, resorption of one partner of a pair of fused semiallogeneic colonies may occur days to weeks after initial contact. The latter process is similar to the degeneration of old individuals, or zooids, that precedes maturation of each new generation of asexual buds. Here we describe comparisons of in vitro reactions of a) mixtures of cells from allogeneic animals and b) cells taken from animals at the zooid-resorption ("takeover") stage of colony development. In vitro autoreactivity of cells from resorbing colonies may reflect in vivo responses to senescent cells, which in turn may be related to allorecognition events that govern fusion or rejection between colonies.

Lymphocyte major histocompatibility complex-encoded class II structures may act as sperm receptors.

Human sperm and blood cells were cocultured in vitro to determine whether specific interactions occur between gametes and blood cells. Evidence for cell type-specific sperm binding and penetration of lymphocytes is presented together with findings that suggest that either or both events involve major histocompatibility complex-encoded class II molecules on lymphocytes and a sperm ligand that is immunoreactive with antibodies to T-cell surface antigen T4. Involvement of HLA-DR is suggested by the pattern of sperm interactions with HLA-DR-positive and -negative cells and by inhibition of sperm binding to HLA-DR-positive cells by a monoclonal antibody that identifies a nonpolymorphic determinant on the HLA-DR molecule. That the complementary sperm ligand may be a T4-like structure is suggested by specific inhibition of sperm-lymphocyte binding with monoclonal antibodies OKT4 and OKT4A. The results are discussed in terms of possible roles for immunoglobulin-related structures in human fertilization and in the sexual transmission of the acquired immunodeficiency syndrome.

Separation and labeling of specific subpopulations of Botryllus blood cells.

Blood cells of the colonial tunicate Botryllus were separated by density gradient centrifugation in Percoll. Unseparated blood cells were used to immunize mice for development of hybridoma cell lines producing anti-Botryllus monoclonal antibodies. These antibodies identify specific subpopulations of blood cells, indicating possible divisions of these cells into defined subgroups sharing particular differentiation antigens. Additional studies utilizing fluorescein- conjugated lectins also revealed differential binding to density- and monoclonal antibody-defined blood cell fractions. These methods allow separation of the different Botryllus blood cell types for functional studies.

Depression of mitogen response in spleen cells from reticuloendotheliosis virus-infected chickens and their suppressive effect on normal lymphocyte response.

Spleen cells and peripheral blood lymphocytes from chickens infected with reticuloendotheliosis virus (REV) were depressed in their responsiveness to phytohemagglutinin (PHA). When spleen cells from uninfected chickens were co-cultured with spleen cells from chickens infected with REV at 2 weeks of age, the PHA response by the normal cells was completely suppressed. Although spleen cells from chickens infected with REV at 6 or 9 weeks of age were also suppressed in their ability to respond to PHA, they did not suppress the mitogenic response of normal cells in mixed cultures.