RESUMO
OBJECTIVE: To compare expression of the serotonin transmembrane transporter (SERT) in normal, early-stage degenerative, and late-stage degenerative canine mitral valve disease. ANIMALS: 24 post-mortem canine mitral valves. METHODS: SERT expression was determined in canine normal (n = 8), early-stage degenerative (n = 8), and late-stage degenerative (n = 8) mitral valves by immunohistochemistry (IHC) and immunoblot (IB) analyses. RESULTS: SERT was expressed in valve interstitial cells of all layers of normal and early-stage degenerative mitral valves based on IHC. SERT was markedly down-regulated in valve interstitial cells, but not valve endothelial cells, of late-stage degenerative mitral valves. SERT expression was significantly decreased in late-stage compared to normal and early-stage degenerative mitral valves based on IB analysis (P < 0.05). CONCLUSIONS: Down-regulation of SERT expression occurs in valve interstitial cells of late-stage, but not early-stage, canine degenerative mitral valves. Down-regulation of SERT could enhance the recently speculated role of serotonin in canine DMVD by decreasing serotonin metabolism and increasing interaction with its receptor. Down-regulation of SERT likely does not play an initiating role in canine DMVD since it does not occur in early-stage disease.
Assuntos
Doenças do Cão/metabolismo , Doenças das Valvas Cardíacas/veterinária , Valva Mitral/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Animais , Doenças do Cão/patologia , Cães , Regulação para Baixo , Feminino , Doenças das Valvas Cardíacas/metabolismo , Doenças das Valvas Cardíacas/patologia , Immunoblotting/veterinária , Masculino , Valva Mitral/patologia , Serotonina/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/biossínteseAssuntos
Ritmo Idioventricular Acelerado/veterinária , Anestesia Intravenosa/veterinária , Anestésicos Intravenosos/efeitos adversos , Doenças do Cão/cirurgia , Propofol/efeitos adversos , Procedimentos Cirúrgicos Refrativos/veterinária , Ritmo Idioventricular Acelerado/induzido quimicamente , Anestesia Intravenosa/efeitos adversos , Animais , Doenças do Cão/induzido quimicamente , Doenças do Cão/fisiopatologia , Cães , Masculino , Procedimentos Cirúrgicos Refrativos/efeitos adversosRESUMO
A 1-year-old domestic longhair cat presented to our hospital with a 4-month history of progressive exercise intolerance and coughing. Two-dimensional echocardiography confirmed the presence of an intrapericardial cyst and cardiac tamponade. Tamponade was relieved via percutaneous aspiration of the cyst. The cyst was surgically excised 4 days later, and histopathology was consistent with a biliary cyst. The cat's clinical signs completely resolved following surgery.
