Functional Characterization of the Gustatory Sensilla of Tarsi of the Female Polyphagous Moth Spodoptera littoralis.

Contact chemoreception is crucial for host plant choice selection in insects and is guided by input from gustatory receptor neurons, GRNs, housed in gustatory sensilla. In this study, the morphology and response spectra of individual tarsal sensilla on the fifth tarsomere of females of the moth Spodoptera littoralis were investigated. Two distinct morphological types of gustatory sensilla, TI and TII, were identified. Extracellular electrophysiological recordings were performed on each sensillum type using three sugars, two bitter substances and salt. Three distinct functional classes (TIα, TIß, TII) were characterized, using cluster analysis based on the response spectra of three of the four responding GRNs. While each functional type of sensillum housed GRNs responding to salt, sugars and bitter compounds, the identity of these cells differed among the functional classes. Interestingly, an interaction between the GRNs responding to sugar and caffeine was found in both TIß and TII sensilla, when binary mixtures were tested. This study provides a functional screening of the tarsal gustatory sensilla, showing a differentiation between sensilla on the tarsi of S. littoralis, providing the female moth with information that can facilitate host plant choice decisions.

Deep-tissue confocal imaging of the central projections of ovipositor sensory afferents in the Egyptian cotton leafworm, Spodoptera littoralis.

The pre-ovipositon behavior of moths is largely dependent upon the cues that a gravid female perceives while assessing potential oviposition sites. Assessment of such sites is accomplished, at least in part, by mechanosensory and gustatory sensilla located on the ovipositor whose sensory neurons project into the terminal abdominal ganglion (TAG). Using anterograde backfill staining, confocal laser scanning microscopy, and three dimensional reconstruction, we traced and analyzed the central projections of the sensory neurons housed in the sensilla located on the ovipositor papillae and explored the neuropilar composition of the TAG in the Egyptian cotton leafworm, Spodoptera littoralis. The TAG consists of three fused neuromeres (6-8th Ner) associated with the 6-8th abdominal segments. Within the TAG, and specifically in the 8th neuromere, four unstructured neuropilar compartments are present; the dorso-ipsilateral motor neuropil (MN), the medio-ipsilateral mechanosensory neuropil (MchN), the medio-ipsilateral small gustatory neuropil (GN), and the medio-contralateral posterior ovipositor glomerulus (Og). The Og appears quite compact, with a hollow core free of terminal arborizations. The MchN is further subdivided into 4 unstructured glomeruli in the 8th neuromere, whose afferents are subsequently extended into 3 glomeruli in the 7th and 6th neuromeres. Few neurites of the Og are populated with large dense varicosities reminiscent of neurosecretory vesicles. Given that all ovipositor nerves converge into a common ganglionic center, the TAG, we assume that this ganglion may be a center for coordination of oviposition behaviors, including movements of the ovipositor during assessment of oviposition substrates and egg laying in S. littoralis.

Spatial organization of antennal olfactory sensory neurons in the female Spodoptera littoralis moth: differences in sensitivity and temporal characteristics.

Single-cell recordings from olfactory sensory neurons (OSNs), housed in sensilla located at the base and at the tip of the antenna, showed selective responses to plant odors and female sex pheromone in this polyphagous moth. A spatial variation existed in sensitivity: OSNs present on the more proximal segment (P) were more sensitive than those on the more distal segment (D). OSNs of the 2 locations also differed in temporal characteristics: OSNs on P had shorter latency and displayed more phasic responses, whereas those on D had more tonic responses, especially at low stimulus concentrations. The 196 OSNs responding to our 35 monomolecular stimuli in the screening were housed in 32 functional sensillum types: 27 in basiconic, 3 in long-trichoid, 2 in coeloconic, and 3 in auricillic sensilla. The OSNs in basiconic, coeloconic, and auricillic sensilla responded to plant-associated odorants, whereas OSNs in long-trichoid sensilla responded to female-produced sex pheromone components. Short-trichoid sensilla showed spontaneous activity, but no responses to any odorant tested. OSN specificity to plant stimuli ranged from highly specific to broadly tuned, but it did not differ clearly from females in more specialized moths. OSN response diversity is discussed in terms of olfactory coding, behavior, and ecological specialization.