RESUMO
OBJECTIVE: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. METHODS: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. RESULTS: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. CONCLUSION: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
Assuntos
Eritrócitos/enzimologia , Testes Hematológicos/normas , Papaína/química , Peptídeo Hidrolases/química , Soluções/normas , Testes de Aglutinação/métodos , Testes Hematológicos/economia , Humanos , Papaína/economia , Peptídeo Hidrolases/economia , Reprodutibilidade dos Testes , Sistema do Grupo Sanguíneo Rh-Hr/química , Sistema do Grupo Sanguíneo Rh-Hr/economia , Soluções/economia , Fatores de TempoRESUMO
ABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
RESUMO Objetivo: Comparar a atividade enzimática de diferentes apresentações de solução de papaína para validação de preparados in-house. Métodos: Foram preparadas duas soluções de papaína, e a terceira apresentação tratou-se de uma solução comercial. Os testes comparativos das reações enzimáticas foram realizados com amostras de hemácias tipadas como RhD fraco. Resultados: As soluções de papaína preparadas in-house apresentaram reatividade enzimática semelhante e estatisticamente sem diferenças em comparação com a atividade enzimática da solução comercial. Conclusão: Avaliando-se a relação entre custo e benefício, as soluções de papaína preparadas in-house são economicamente vantajosas, podendo ser incorporadas às rotinas imuno-hematológicas como forma de enfrentamento em períodos de crise financeira e em políticas de retenção de gastos.
Assuntos
Humanos , Peptídeo Hidrolases/química , Soluções/normas , Papaína/química , Eritrócitos/enzimologia , Testes Hematológicos/normas , Peptídeo Hidrolases/economia , Sistema do Grupo Sanguíneo Rh-Hr/economia , Sistema do Grupo Sanguíneo Rh-Hr/química , Soluções/economia , Fatores de Tempo , Testes de Aglutinação/métodos , Papaína/economia , Reprodutibilidade dos Testes , Testes Hematológicos/economiaRESUMO
BACKGROUND: Investigation of erythrocyte antigens in New World monkeys, especially in the Brazilian ones, is scant and incomplete. METHODS: Determining the presence of 29 erythrocyte antigens from 11 human blood group systems (ABO, H, Rh, Kell, Duffy, Kidd, Lewis, P, MNS, Lutheran and Diego) on erythrocytes in nine Capuchin monkeys (Sapajus sp.). RESULTS: A majority (20 of 29) of human erythrocyte antigens were not found in this monkey genus. Erythrocyte phenotyping was very similar within this animal group, as five Capuchin monkeys differed from the other four in the ABO system only. CONCLUSION: The erythrocyte phenotype for this group of animals is less diversified than in humans. Some monkey erythrocyte antigens were similar in frequency, whereas others were different from those observed in human ethnicities.
