[New bacterial microorganisms in the etiology of human infections. The genus Gardnerella]. / Noi microorganisme bacteriene în etiologia infectiilor umane. Genul Gardnerlla.

Haemophilus vaginalis first affiliated in the bacterial systems in the genus Haemophilus, the Corynebacterium due to its morphophysiological characteristics--forms a new genus Gardnerella with only one species G. vaginalis. The bacterium is important for man's pathology, as it is responsible for the most numerous vaginitis, considered nonspecific. The paper reports synthetically the present knowledge in biology, pathogenicity, diagnosis and treatment.

[Periodontal diseases with Entamoeba gingivalis]. / Parodontopatii cu Entamoeba gingivalis.

At the beginning of our century Entamoeba gingivalis was considered to be a pathogenic bacteria, capable to induce parodontal lesions. Later on it was also found in healthy persons, and the germ was less interesting from the medical view-point. In the present study the authors report their findings concerning E. gingivalis in 135 patients with various stomatological affections including: dental caries, parodontopathies, pulpitis, gangrene, ulcero-necrotic stomatitis etc. The study was started following the discovery of the amoeba in the gingival exsudate of a male aged 19 years with chronic superficial marginal parodontopathy, who, after a treatment with metronidazol, was cured. Entamoeba gingivalis belongs to the Rhizopoda class, together with E. dysenteriae, and E. coli, but, in contrast with these strains it does not have resistance forms (cysts). Oral amoeba were evidenced in 18 out of 78 patients with parodontal lesions (23.07%), in the gingival exsudate, the purulent secretion from parodontal pouches, in the dental tartar, the alveolar fluid following extraction etc. In 117 students from the Faculty of Stomatology, and in 57 patients with various other stomatological affections these germs were not found in any of the abovementioned products. Microscopic examination of fresh preparations, and of Giemsa-stained smears was the main method for the detection of the amoeba. The etiopathogenic role of E. gingivalis is re-examined in discussions regarding certain parodontopathies.

[Comparative study of immunofluorescence, coagglutination and urinary immunoglobulins in the diagnosis of the localization of urinary infections]. / Studiul comparativ al imunofluorescentei, coaglutinarii si imunoglobulinelor urinare în diagnosticul de localizare a infectiei urinare.

Three laboratory methods -- immunofluorescence, coagglutination and urinary immunoglobulin determinations -- were applied in 16 patients with urinary infections, of whom 12 with pyelonephritis and 4 with cystitis. There was an 100% agreement between the three tests in the pyelonephritis cases. In the cystitis cases, there was 100% agreement between immunofluorescence and urinary immunoglobulin and 50% between the three methods. Urinary immunoglobulin determination and coagglutination may be recommended as routine auxiliary clinical laboratory methods for early detection of renal lesions and localization of the infection.

[Studies of oral microbiocenosis. III. Incidence of microorganisms of the Mycoplasma genus]. / Studii de microbiocenoza orala. Nota III. Incidenta microorganismelor din genul Mycoplasma.

Microorganisms of the Mycoplasma genus were found in the washing fluids of the oral cavity in 2% of healthy youths, and in 57.1% of patients with parodontal lesions: 25.7% in the buccal fluid, 17.1% in the dental tartar and 34.2% in the gingiva exsudate. Microplasma was not found in dental caries and in the radicular canals with pulpar gangrene. Although these microorganisms were frequently found in subjects with parodontal lesions they cannot be implicated with certainty in their aetiology.

[10 years after the death of Professor Mihai Ciuca (18 July 1883--20 Feb 1969). Mihai Ciuca and the study of bacteriophage, recorded in his working notebooks]. / La 10 ani de la moartea profesorului Mihai Ciuca (18. VII. 1883--20.II. 1969). Mihai Ciuca si studiul bacteriofagului, însemnari în caietele de lucru.

The work records of professor Mihai Ciuca (found in the archives of the Department of Microbiology) give us an insight into his scientific thoughts and practical activity. These documents speak of his dedication to the problem of the bacteriophage started in Jules Bordet's laboratory of the Pasteur Institute in Brussels, in 1920 and continued until 1969, the year in which he died. Since the discovery of the phenomenon of lysogeny, called the Bordet-Ciuca phenomenon, the professor initiated a wide range of research themes on the bacteriophage, guiding and promoting the work of a great number of specialists in the National Center, founded in 1949.

[Studies of oral microbiogenesis. II. Significance of staphylocci present in periodontal lesions, pulp gangrene and in the alveolus after tooth extraction]. / Studii de microbiogenoza orala. II. Semnificatia stafilococilor prezenti în leziunile parodontale, gangrena pulpara si în alveola dupa extractia dentara.

A study was carried out on the properties and significance of staphylococci isolated from the alveoli after dental extraction (12 strains), from parodontal pus (19 strains) and pulpar gangrene (15 strains). By their frequency, staphylococci of the Staphylococcus aureus have an etiologic significance in pulpar gangrene. Of particular importance for the diagnosis and epidemiology was the predominance of lysotype 80 among the staphylococci isolated from pulpar gangrene and lysotype 77 in healthy carriers. In 2 of 11 patients the coexistence of the same lysotype was found in the pulpar gangrene and nasal secretion.

[Antibiograms of Leptospira on solid media in Petri dishes]. / Antibiograma leptospirelor pe medii solide în placi Petri.

The sensitivity of fowl leptospirae to antibiotics was tested by diffusiometry on solid media in Petri dishes. The method includes two stages: 1) visible leptospirae cultures are obtained by central seeding and incubation during 3--5 days at 28 or 37 degrees C; 2) antibiotic tablets are disposed at 2 cm from the visible edge of the multiplication zone of the leptospirae. The edge is marked with a pencil at the moment in which the antibiotics are applied. Incubation is continued at the same temperatures. The results are read after five days taking as reference the position of the marginal multiplication line. The sensitivity of leptospirae to antibiotics is expressed by arrested multiplication and resistance by advance of the marginal multiplication line up to or even beyond the antibiotic tablets. Fowl leptospirae are sensitive to penicillin, tetracyclin, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, erythromycin, and resistant to mycostatin, septrin, rifampicin, optoquine, sulphatiazol.

[Studies of oral microbiocenosis. I. Incidence and properties of staphylocci in young persons]. / Studii de microbiocenoza orala. I. Frecventa si proprietatile stafilococilor la tineri

The study of staphylococci in the buccodental washings of 117 second year students at the Faculty of Stomatology, all healthy youths aged 20-25 years, gave the following results: (I) stapphylococci were present in 90% of the students; (II) 50.66% were oral carriers of pathogenic staphylococcal strains; (III) in 23 cases two or more staphylococcal strains were found with different metabolic and biologic properties; (IV) part of the bucal lysozyme was of microbial origin since 95% of the coagulasopositive strains and 88% of the coagulasonegative strains produce lysozymes; (V) 35.23% of the strains yield staphylococcal active with regard to M. lysodeicticus; (VI) 56.19% of the staphylococcal strains were resistant to penicillin and/or tetracyclin, and 5 coagulopositive strains developed in media with a penicillin concentration of 800 u/ml; (VII) 85% of the coagulopositive staphylococci were sensitive to human anti-staphylococcal bacteriophages, the most frequent being phage type 77 of group III.