[The perspectives of development of molecular diagnostics of allergy in the microarray format].

All over the world, many people suffer of various forms of allergy. The timely and accurate diagnostics of allergic sensitization is able to prevent the complications and to select a proper mode of therapy. Over the last century, were isolated and characterized hundreds of specific allergenic substances, in the form of extract mainly In 1966, the class E immunoglobulins were discovered and their role in the formation of hypersensitivity type 1 was explained. Hence, the application of techniques of specific sensitization diagnostics became possible in vitro. The diagnostic systems based on the principles of immune-enzyme assay on plate. The appearance and rapid development of microarray technology in the end of XX century provided an opportunity to implement mass screening of specimen on hundreds and thousands markers. The given technique seems to be promising for carrying out a voluminous and rapid allergoscreening. A prototype specimen of test system was developed in the form of microarray with 15 immobilized allergens. The 16 samples of blood serum of patients with allergic diseases were analyzed. The findings demonstrated a high correlation with reference allergosorbent test. The analysis mode implies a direct immobilization of allergen on phase. This approach makes the most of standard extracts unsuitable due to the presence of considerable amount of non-allergenic "ballast" proteins. In most cases, certain groups of allergens, as shared proteins, manifest a high cross-reactivity decreasing the diagnostics specificity. The issue of appropriateness and necessity of implementing the refined or artificial allergenic components is to be discussed. The experiment results confirmed the correctness of conclusions done and approaches chosen. The component-enabling diagnostics in the microarray format will make it possible to enhance the specificity and accessibility of analysis which will undoubtedly affect patients' quality of life.

[Polymorphisms of genes of rennin-angiotensine system and their correlation with psychological manifestations of birth stress].

Study of correlation of polymorphisms of genes of the rennin-angiotensine system--insertion-deletion (I/D) polymorphism of the angiotensine-converting enzime (ACE), and the polymorphism of angiotensinogen (AGT), consisting in submission of T into C in the 704 position (M235T)--with various characteristics of psychological functioning and clinical characteristics in women giving birth, was conducted. The group consisted of 56 young female Ss, without chronic diseases in the anamnesis, no complication in the course of pregnancy, birth normal and timely, state of the child normal in all cases. Basing on factor analysis of 7 psychological induces, providing integral assessment of state of the Ss in the course la late pregnancy, giving birth, and the immediately following post partum period, statistically reliable correlation between such first-rate psychological process as creativity, and polymorphism of the gene of angiotensinogene, was demonstrated, as well as difference in distribution of genotypes between the group of women giving birth, and general population. Statistically relevant correlation between presence of the deletion (D) allele of the ACE gene, and heightened level of neuroticization, reported by us earlier, was observed in this case, as well. No significant links between religiosity/spirituality, and genetic data was found. These results are in concordance with present-day views concerning the role of the rennin-angiotensine system in the providing of active adaptation to stressful conditions; they tend to provide fresh outlook upon the comparative study of input of heredity, and education, into the ontogenesis of higher psychological functions in normal humans.