1.
Nucleosides Nucleotides Nucleic Acids
; 20(4-7): 1371-5, 2001.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11563025
RESUMO
Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2 + 2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.