Setting qualitative performance parameters of elite surfing aerial manoeuvres with 360° rotation / Establecimiento de parámetros cualitativos de rendimiento de maniobras aéreas de surf de élite con rotación de 360 grados / Definição de parâmetros qualitativos de desempenho de manobras aéreas de surf de elite com rotação de 360 graus

El objetivo fue analizar parámetros de maniobras aéreas de surf de alto nivel, apoyando el entrenamiento específico y la prevención de lesiones. Utilizando análisis de video de maniobras aéreas completadas porsurfistas masculinos de top-5en 19 eventos durante las temporadas 2018y 2019del World Championship Tour, se seleccionaron (n=97): Frontside Air, Frontside Air Reverse 360, Backside Air y Backside Air Reverse 360.Se trata de una investigación de métodomixto. Tiene un análisis de tarea cualitativo, realizado a través del análisisen fases, considerando características clave de las acciones corporales y principios de movimiento. A través de la metodologíaobservacional (nomotético, puntual y multidimensional) se midió laaltura del aéreo, el ancho del BoS y los ángulos de flexión de la rodilla y del tobillo delanteros.Las referencias en el análisis cualitativo fueron: rotación de cabeza y tronco, triple flexión de miembros inferiores (preparación) y máspronunciada en larecuperación; flexión de cabeza, tronco y brazos desde el despegue (preparación) hasta el pico del vuelo (principal) provocando el desplazamiento anterosuperior del CoM; ampliación de la BoS para aéreos frontales (principal). El análisiscuantitivo permitió identificarlamedia dela altura del aéreo (169,60±52,85cm), del ancho de la base de sustentación (BoS) (79,17±13,72cm), y de los ángulos de flexión de la rodilla (113,78±19,90º) y del tobillo(32,74±14,51º)delanteros durante el aterrizaje. El análisis descriptivo puede contribuir al desarrollo de programas de formación específicos para la enseñanza y el aprendizaje, con el objetivo de mejorar el rendimiento de las maniobras aéreas. (AU)

Purpose was to analyse parameters of high-level surfing aerial manoeuvres, supporting specific training and injury prevention. Using video analysis ofaerials completed by top-5male surfersin 19 events during 2018and 2019 seasonsof the World Championship Tour, were selected (n=97): Frontside Air, Frontside Air Reverse 360, Backside Air and Backside Air Reverse 360. This is a mixed method research. Has a qualitative task analysis, carried out through phaseanalysis, considering key features from body actions and principles of movement. Through observational methodology (nomothetic, single point and multidimensional)was measured the aerial height, theBoS width, and flexion angles of front knee and front ankle.Qualitative analysis references were: rotation of the head and trunk, triple-flexion of the lower limbs (retraction) and more pronounced in the follow-through; head, trunk, and arms flexion fromthe take-off (retraction) to the peak of the flight (action) causing the anterosuperior CoM displacement; widening of the BoS for frontside aerials (action). Quantitative analysis allowed to identify the mean of aerial height (169.60±52.85cm), of base of support (BoS) width (79.17±13.72cm), and flexion anglesof front knee (113.78±19.90º) and front ankle (32.74±14.51º) during landing.Descriptive analysis may contribute to the development of specific training programs for teaching and learning, aiming to improve performance of aerial manoeuvres. (AU)

O objetivo foi analisar parâmetros de manobras aéreas de surf de alto nível, dando suporte ao treino específico e à prevenção de lesões. Através da análisede vídeode manobras aéreas concluídaspor surfistas masculinos do top-5,em 19 eventos durante as temporadas 2018 e 2019do World Championship Tour, foram selecionados (n=97): Frontside Air, Frontside Air Reverse 360, Backside Air e Backside Air Reverse 360.Esteé um estudo com um método misto. Temos uma análise da tarefa qualitativa, realizada através da análise por fases, considerando as principais características das ações corporais e dos princípios do movimento.Através da metodologia observacional (nomotético, pontual e multidimensional) foi medida a altura do aéreo, a largura do BoS e os ângulos de flexão do joelho e do tornozelodianteiros. As referências da análise qualitativa foram: arotação de cabeça e tronco, tripla flexão dos membros inferiores (preparação) e mais acentuada na finalização; flexão da cabeça, tronco e braços desde a descolagem (preparação) até o pico do voo (ação principal) causando o deslocamento antero-superior do CoM; alargamento da BoS paraaéreos frontais (ação principal). A análise quantitativa permitiu identificar a média da altura do aéreo (169,60±52,85cm), da largura da base de sustentação (BoS) (79,17±13,72cm) e dos ângulos de flexão do joelho(113,78±19,90º) e tornozelo (32,74±14,51º)dianteiros durante a aterragem. A análise descritiva pode contribuir para o desenvolvimento de programas de treino específicos para ensino e aprendizagem, visando melhorar o desempenho das manobras aéreas. (AU)

The VD1/RPD2 α1-neuropeptide is highly expressed in the brain of cephalopod mollusks.

In certain gastropod mollusks, the central neurons VD(1) and RPD(2) express a distinct peptide, the so-called VD(1)/RPD(2) α1-neuropeptide. In order to test whether this peptide is also present in the complex cephalopod central nervous system (CNS), we investigated several octopod and squid species. In the adult decapod squid Idiosepius notoides the α1-neuropeptide is expressed throughout the CNS, with the exception of the vertical lobe and the superior and inferior frontal lobes, by very few immunoreactive elements. Immunoreactive cell somata are particularly abundant in brain lobes and associated organs unique to cephalopods such as the subvertical, optic, peduncle, and olfactory lobes. The posterior basal lobes house another large group of immunoreactive cell somata. In the decapod Idiosepius notoides, the α1-neuropeptide is first expressed in the olfactory organ, while in the octopod Octopus vulgaris it is first detected in the olfactory lobe. In prehatchlings of the sepiolid Euprymna scolopes as well as the squids Sepioteuthis australis and Loligo vulgaris, the α1-neuropeptide is expressed in the periesophageal and posterior subesophageal mass. Prehatchlings of L. vulgaris express the α1-neuropeptide in wide parts of the CNS, including the vertical lobe. α1-neuropeptide expression in the developing CNS does not appear to be evolutionarily conserved across various cephalopod taxa investigated. Strong expression in different brain lobes of the adult squid I. notoides and prehatching L. vulgaris suggests a putative role as a neurotransmitter or neuromodulator in these species; however, electrophysiological evidence is still missing.

Analysis of neurotransmitter distribution in brain development of benthic and pelagic octopod cephalopods.

The database on neurotransmitter distribution during central nervous system development of cephalopod mollusks is still scarce. We describe the ontogeny of serotonergic (5-HT-ir) and FMRFamide-like immunoreactive (Fa-lir) neurons in the central nervous system of the benthic Octopus vulgaris and Fa-lir distribution in the pelagic Argonauta hians. Comparing our data to previous studies, we aim at revealing shared immunochemical domains among coleoid cephalopods, i.e., all cephalopods except nautiluses. During development of O. vulgaris, 5-HT-ir and Fa-lir elements occur relatively late, namely during stage XII, when the brain neuropils are already highly differentiated. In stage XII-XX individuals, Fa-lir cell somata are located in the middle and posterior subesophageal mass and in the optic, posterior basal, and superior buccal lobes. 5-HT is predominately expressed in cell somata of the superior buccal, anterior basal, and optic lobes, as well as in the subesophageal mass. The overall population of Fa-lir neurons is larger than the one expressing 5-HT. Fa-lir elements are distributed throughout homologous brain areas of A. hians and O. vulgaris. We identified neuronal subsets with similar cell number and immunochemical phenotype in coleoids. These are located in corresponding brain regions of developmental stages and adults of O. vulgaris, A. hians, and the decapod squid Idiosepius notoides. O. vulgaris and I. notoides exhibit numerous 5-HT-ir cell somata in the superior buccal lobes but none or very few in the inferior buccal lobes. The latter have previously been homologized to the gastropod buccal ganglia, which also lack 5-HT-ir cell somata in euthyneuran gastropods. Among coleoids, 5-HT-ir neuronal subsets, which are located ventrally to the lateral anterior basal lobes and in the anterior middle subesophageal mass, are candidates for homologous subsets. Contrary to I. notoides, octopods exhibit Fa-lir cell somata ventrally to the brachial lobes and 5-HT-ir cell somata close to the stellate ganglia.

Effect of the nutritional status of semi-continuous microalgal cultures on the productivity and biochemical composition of Brachionus plicatilis.

The rotifer Brachionus plicatilis was cultured using the microalga Isochrysis aff. galbana clone T-ISO as feed. T-ISO was cultured semi-continuously with daily renewal rates of 10%, 20%, 30%, 40%, and 50% of the volume of cultures. The increase of renewal rate led to increasing nutrient and light availability in microalgal cultures, which caused differences in the biochemical composition of microalgal biomass. Growth rate, individual dry weight, organic content, and biomass productivity of rotifer cultures increased in response to higher growth rate in T-ISO cultures. Rotifer growth rate showed a strong negative correlation (R² = 0.90) with the C/N ratio of microalgal biomass. Rotifer dry weight was also affected by nutrient availability of T-ISO cultures, increasing up to 50% from nutrient-limited to nutrient-sufficient conditions. Consequently, biomass productivity of rotifer cultures increased more than twofold with the increase of renewal rate of T-ISO cultures. Rotifer organic content underwent the same trend of total dry weight. Maximum content of polyunsaturated fatty acids was reached in rotifers fed T-ISO from the renewal rate of 40%, with percentages of docosahexaenoic acid (22:6ω-3, DHA) and eicosapentaenoic acid (20:5ω-3, EPA) of 11% and 5% of total fatty acids, respectively. Selecting the most appropriate conditions for microalgal culture can therefore enhance the nutritive quality of microalgal biomass, resulting in a better performance of filter feeders and their nutrient content, and may constitute a useful tool to improve the rearing of fish larvae and other aquaculture organisms that require live feed in some or all the stages of their life cycle.

Enriching rotifers with "premium" microalgae. Nannochloropsis gaditana.

The nutritive quality of Nannochloropsis gaditana cultured semicontinuously with different daily renewal rates was tested as a diet for short-term enrichment of the rotifer Brachionus plicatilis. After 24 h, dramatic differences in the survival, dry weight, and biochemical composition of the rotifers depending on the renewal rate of microalgal cultures were observed. Survival after the feeding period increased with increasing renewal rates. Rotifers fed microalgae from low renewal rate, nutrient-deficient cultures showed low dry weight and organic contents very similar to those of the initial rotifers that were starved for 12 h before the start of the feeding period. On the contrary, rotifers fed nutrient-sufficient microalgal cells underwent up to twofold increases of dry weight and protein, lipid, and carbohydrate contents with regard to rotifers fed nutrient-depleted N. gaditana. Consequently, feed conversion rate decreased in these conditions, indicating a better assimilation of the microalgal biomass obtained at high renewal rates. No single microalgal biochemical parameter among those studied can explain the response of the filter feeder. Similarly to gross composition, EPA and n-3 contents in rotifers fed microalgae from nutrient-sufficient cultures were double than the contents found in rotifers fed nutrient-limited microalgae. In addition, very high positive correlations between the contents of EPA and n-3 in N. gaditana and B. plicatilis were observed. These results demonstrate that selecting the appropriate conditions of semicontinuous culture can strongly enhance the nutritional value of microalgae that is reflected in the growth and biochemical composition of the filter-feeder even in short exposure periods.